[1]于雅洁,曹鹏辉,宋云生,等.江苏太湖地区粳稻稻瘟病抗性基因(Pi2、Pita、Pib)的分布及抗病效应[J].江苏农业学报,2024,(05):769-776.[doi:doi:10.3969/j.issn.1000-4440.2024.05.001]
　YU Yajie,CAO Penghui,SONG Yunsheng,et al.Distribution and resistant effects of blast resistant genes (Pi2, Pita, Pib) in japonica rice in Taihu area of Jiangsu province[J].,2024,(05):769-776.[doi:doi:10.3969/j.issn.1000-4440.2024.05.001]
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江苏太湖地区粳稻稻瘟病抗性基因(Pi2、Pita、Pib)的分布及抗病效应()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2024年05期
页码:
769-776
栏目:
遗传育种·生理生化
出版日期:
2024-05-30

文章信息/Info

Title:
Distribution and resistant effects of blast resistant genes (Pi2, Pita, Pib) in japonica rice in Taihu area of Jiangsu province
作者:
于雅洁; 曹鹏辉; 宋云生; 陈飞; 乔中英; 董明辉; 朱勇良; 谢裕林; 袁彩勇
(江苏太湖地区农业科学研究所,江苏苏州215104)
Author(s):
YU Yajie; CAO Penghui; SONG Yunsheng; CHEN Fei; QIAO Zhongying; DONG Minghui; ZHU Yongliang; XIE Yulin; YUAN Caiyong
(Jiangsu Taihu Area Institute of Agricultural Sciences, Suzhou 215104, China)
关键词:
稻瘟病; PARMS技术; 抗性基因; 抗病育种
Keywords:
rice blast; penta-primer amplification refractory mutation system (PARMS) technology; resistance genes; disease-resistance breeding
分类号:
S511;S435.111.4+1
DOI:
doi:10.3969/j.issn.1000-4440.2024.05.001
摘要:
为分析稻瘟病抗性基因Pi2、Pita、Pib在江苏太湖地区粳稻稻瘟病抗性育种中的作用,利用五引物扩增受阻突变体系(PARMS)检测技术,检测水稻稻瘟病抗性基因Pi2、Pita、Pib在江苏太湖地区农业科学研究所筛选培育的799份高世代稳定试验材料中的分布情况,并结合穗颈瘟人工接种鉴定结果分析基因型与抗性的关系。结果表明,在799份供试水稻品种中,以抗性基因 Pib的分布频率最高,抗性基因 Pita的分布频率也相对较高,抗性基因 Pi2分布频率较低,多数材料携带2个抗性基因；在抗性基因组合中,Pita+Pib组合检出率较高,为44.81%(358/799)。田间抗病性鉴定结果表明,当供试材料中只存在单一抗病基因时,中抗级别以上材料占比为33.60%(86/256),当供试材料中存在复合基因型时,中抗级别以上材料占比为61.02%(299/490),表明抗性基因共存可以有效增强植株对稻瘟病的抗性。本研究结果为江苏太湖地区粳稻稻瘟病抗性基因聚合育种的选择提供了理论支持。
Abstract:
In order to analyze the role of rice blast resistance genes Pi2, Pita and Pib in japonica rice blast resistance breeding in areas of Taihu area of Jiangsu province, penta-primer amplification refractory mutation system (PARMS) detecting technology was used to detect the distribution of rice blast resistance genes Pi2, Pita and Pib in 799 high generation stable test materials screened and bred by Jiangsu Taihu Area Institute of Agricultural Sciences, and to analyze the relationship between genotype and resistance in combination with the results of artificial inoculation and identification of seedling blast and neck panicle blast. The results showed that among the 799 test materials, most of them carried two resistance genes,the distribution frequency of resistance gene Pib was the highest, the distribution frequency of resistance gene Pita was high, and the distribution frequency of resistance gene Pi2 was low. In the resistance gene combination, the detection rate of Pita+Pib combination was relatively high, which was 44.81% (358/799). The results of field disease resistance identification showed that when there was only one single disease resistance gene in the test materials, materials with medium resistance or above accounted for 33.60% (86/256). When there were composite genotypes in the test materials, materials with medium resistance or above accounted for 61.02% (299/490), indicating that the coexistence of resistance genes could effectively enhance the resistance to rice blast. The results provide theoretical support for the selection of japonica rice blast resistance gene polymerization breeding in the Taihu region of Jiangsu province.

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备注/Memo

备注/Memo:
收稿日期：2024-01-23基金项目：江苏省“333”高层次人才培养项目；苏州市科技计划项目（SNG2022012）；江苏太湖地区农业科学研究所院基金项目（21008）作者简介：于雅洁（1995-），女，山东威海人，硕士研究生，研究实习员，主要从事水稻遗传育种的研究。（E-mail）781911361@qq.com通讯作者：袁彩勇,（E-mail）hysdycy@163.com
更新日期/Last Update: 2024-07-13