[1]史文军,王学江,李峰,等.饲料中添加褐藻寡糖对脊尾白虾免疫能力的影响[J].江苏农业学报,2024,(04):698-710.[doi:doi:10.3969/j.issn.1000-4440.2024.04.014]
　SHI Wen-jun,WANG Xue-jiang,LI Feng,et al.Effects of alginate oligosaccharides supplementation on immunity of Exopalaemon carinicauda[J].,2024,(04):698-710.[doi:doi:10.3969/j.issn.1000-4440.2024.04.014]
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饲料中添加褐藻寡糖对脊尾白虾免疫能力的影响()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2024年04期
页码:
698-710
栏目:
畜牧兽医·水产养殖·益虫饲养
出版日期:
2024-04-30

文章信息/Info

Title:
Effects of alginate oligosaccharides supplementation on immunity of Exopalaemon carinicauda
作者:
史文军1; 2; 3; 王学江4; 李峰4; 刘正一1; 3; 迟艳4; 张志凯4; 黎慧2; 王李宝2; 孙林1; 万夕和2; 秦松1; 3
(1.中国科学院烟台海岸带研究所,山东烟台264003；2.江苏省海洋水产研究所,江苏南通226007；3.中国科学院大学,北京100049；4.五洲丰农业科技有限公司,山东烟台264000)
Author(s):
SHI Wen-jun1; 2; 3; WANG Xue-jiang4; LI Feng4; LIU Zheng-yi1; 3; CHI Yan4; ZHANG Zhi-kai4; LI Hui2; WANG Li-bao2; SUN Lin1; WAN Xi-he2; QIN Song1; 3
(1.Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China；2.Jiangsu Institute of Oceanology & Marine Fisheries, Nantong 226007, China；3.University of Chinese Academy of Sciences, Beijing 100049, China；4.Wuzhoufeng Agricultural Science & Technology Co., Ltd., Yantai 264000, China)
关键词:
脊尾白虾; 褐藻寡糖; 二尖梅奇酵母; 免疫能力
Keywords:
Exopalaemon carinicauda; alginate oligosaccharides; Metschnikowia bicuspidate; immunity
分类号:
S917.4
DOI:
doi:10.3969/j.issn.1000-4440.2024.04.014
摘要:
为了探究饲料中添加褐藻寡糖(AOS)对脊尾白虾(Exopalaemon carinicauda)免疫和抵抗二尖梅奇酵母(Metschnikowia bicuspidate)能力的影响,试验设置4个不同褐藻寡糖添加量处理,分别为0 mg/kg(CK)、500 mg/kg(T1)、1 000 mg/kg(T2)和2 000 mg/kg(T3),在相同条件下饲养60 d。结果表明:T2处理脊尾白虾的终末质量、总质量增长率和特定生长率极显著高于CK(P＜0.01)；T3处理脊尾白虾存活率极显著高于CK(P＜0.01)。相较于CK,T2处理脊尾白虾肝胰腺中SOD活性、肝胰腺和肌肉中ACP活性、肝胰腺和肌肉中AKP活性、肝胰腺中PO活性均极显著提高(P＜0.01)。T1处理LGBP基因在脊尾白虾肝胰腺中相对表达量极显著高于CK(P＜0.01),T2处理SOD、LGBP基因在脊尾白虾肝胰腺中相对表达量均极显著高于CK(P＜0.01),各处理LZM、SR和CTSB基因在脊尾白虾肝胰腺中相对表达量与CK均无显著差异(P>0.05)。表明饲料中添加1 000 mg/kg褐藻寡糖可以有效提高脊尾白虾体内部分抗氧化和免疫相关基因的表达量,提高脊尾白虾的抗氧化水平和免疫能力。基因相对表达量检测结果与酶活性检测结果一致。攻毒试验中,T1、T2和T3处理脊尾白虾存活率在第3～5 d均极显著高于CK(P＜0.01),但各处理脊尾白虾最终存活率和CK相同,表明褐藻寡糖对二尖梅奇酵母MQ2101具有一定的防控作用,但并不能提高感染后的最终存活率。
Abstract:
In order to investigate the effects of alginate oligosaccharides (AOS) on the immunity and resistance of Exopalaemon carinicauda to Metschnikowia bicuspidate, four treatments were set up, the addition levels of AOS were 0 mg/kg (CK), 500 mg/kg (T1), 1 000 mg/kg (T2) and 2 000 mg/kg (T3), respectively. The shrimps were fed under the same conditions for 60 days. The results showed that the terminal weight, total weight growth rate and specific growth rate of Exopalaemon carinicauda of T2 treatment were significantly higher than those of CK (P＜0.01), and the survival rate of Exopalaemon carinicauda of T3 treatment was significantly higher than that of CK (P＜0.01). Compared with CK, the SOD activity in hepatopancreas, ACP activity in hepatopancreas and muscles, AKP activity in hepatopancreas and muscles, and PO activity in hepatopancreas of Exopalaemon carinicauda of T2 treatment were significantly increased (P＜0.01). The relative expression level of LGBP in hepatopancreas of Exopalaemon carinicauda of T1 treatment was significantly higher than that of CK (P＜0.01). The relative expression levels of SOD and LGBP in hepatopancreas of Exopalaemon carinicauda of T2 treatment were significantly higher than those of CK (P＜0.01). The relative expression levels of LZM, SR and CTSB genes in hepatopancreas of Exopalaemon carinicauda were not significantly different from those of CK (P>0.05). The results showed that the addition of 1 000 mg/kg alginate oligosaccharides in the diet could effectively increase the expression of some anti-oxidation and immune related genes, and enhance the anti-oxidation level and immune ability of Exopalaemon carinicauda. The results of relative gene expression were consistent with the results of enzyme activity detection. In the challenge experiment, the survival rate of Exopalaemon carinicauda in T1, T2 and T3 treatments was significantly higher than that in CK (P＜0.01) in 3-5 d. But the final survival rate of Exopalaemon carinicauda in each treatment was the same as that in CK, which indicated that alginate oligosaccharides could prevent and control Metschnikowia bicuspidate MQ2101, but could not improve the final survival rate after infection.

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备注/Memo

备注/Memo:
收稿日期:2023-03-07基金项目:江苏省农业重大新品种创制项目(PZCZ201747)；江苏省农业科技自主创新基金项目[CX(22)3083]；江苏省种业振兴揭榜挂帅项目[JBGS(2021)122]；江苏现代农业产业技术体系资助项目[JATS(2022)419、JATS(2022)164]作者简介:史文军(1987-),男,安徽寿县人,博士,副研究员,主要从事海水虾类品种选育和绿色健康养殖研究。(E-mail)muzhiye080326@126.com通讯作者:万夕和,(E-mail)wxh1708@163.com；秦松,(E-mail)sqin@yic.ac.cn
更新日期/Last Update: 2024-05-22