[1]吴雅杰,樊炳君,朱国兴,等.特基拉芽孢杆菌KC 121拮抗玉米镰刀菌的防病促生作用[J].江苏农业学报,2024,(02):233-242.[doi:doi:10.3969/j.issn.1000-4440.2024.02.005]
　WU Ya-jie,FAN Bing-jun,ZHU Guo-xing,et al.Disease prevention against corn Fusarium and growth promotion of Bacillus tequilensis KC 121[J].,2024,(02):233-242.[doi:doi:10.3969/j.issn.1000-4440.2024.02.005]
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特基拉芽孢杆菌KC 121拮抗玉米镰刀菌的防病促生作用()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2024年02期
页码:
233-242
栏目:
植物保护
出版日期:
2024-02-25

文章信息/Info

Title:
Disease prevention against corn Fusarium and growth promotion of Bacillus tequilensis KC 121
作者:
吴雅杰1; 樊炳君2; 朱国兴1; 焦钰1; 张兴丽1; 王瑞雪1; 周萍1; 曹艳茹1
(1.昆明学院农学与生命科学学院，云南昆明650214；2.云南丽江市植保植检站，云南丽江674199）
Author(s):
WU Ya-jie1; FAN Bing-jun2; ZHU Guo-xing1; JIAO Yu1; ZHANG Xing-li1; WANG Rui-xue1; ZHOU Ping1; CAO Yan-ru1
(1.College of Agriculture and Life Sciences, Kunming University, Kunming 650214, China;2.Lijiang Plant Protection Station, Lijiang 674199, China)
关键词:
特基拉芽孢杆菌; 镰刀菌; 抑菌机理; 促生机理
Keywords:
Bacillus tequilensis; Fusarium; antibacterial mechanism; promoting growth mechanism
分类号:
S435.131
DOI:
doi:10.3969/j.issn.1000-4440.2024.02.005
摘要:
为初步探究1株分离自兰坪铅锌尾矿的特基拉芽孢杆菌KC 121的防病促生效果及作用机理，将其无菌滤液涂布平板与病原菌共培养以及与发酵滤液病原菌孢子共孵育来测定菌株KC 121对病原菌菌丝生长、产孢数量及孢子萌发的影响；以发酵液浸种试验来测定菌株KC 121对玉米种子萌发的影响；最后利用盆栽灌根的方法研究菌株KC 121对玉米镰刀菌根腐病的防治效果及对玉米幼苗的促生作用并探究其机理。结果表明，特基拉芽孢杆菌KC 121无菌滤液可使镰刀菌菌丝发生皱缩和凹陷，同时抑制了镰刀菌孢子的产生和萌发；浸种试验结果表明，稀释1×103倍的菌株KC 121发酵液可以促进玉米种子萌发时主根增长61%，芽长增加162%；在盆栽试验中，菌株KC 121发酵液原液对腐皮镰刀菌（Fusarium solani）和拟枝孢镰刀菌（Fusarium sporotrichioides）引起的玉米根腐病的防治效果分别为66.67%和76.00%，且分别使玉米植株地上部分干质量和鲜质量提高了130%和90%。促生机理探究发现特基拉芽孢杆菌KC 121通过产淀粉酶，并产生6.19 ng/ml的吲哚乙酸（IAA）和提高玉米幼苗的总叶绿素含量来促进玉米幼苗生长。研究结果显示特基拉芽孢杆菌KC 121对防治玉米镰刀菌病害及促进玉米生长具有应用潜力。
Abstract:
The Bacillus tequilensis KC 121 which isolated from the lead-zinc tailings of Lanping, showed strong inhibition activity against Fusarium spp. In this article, its fungal inhibition activity, plant growth promotion effect, and the corresponding mechanism were studied. The sterile filtrate of KC 121 was used to co-culture with pathogenic fungi and co-incubation with pathogenic fungal spores to determine its effects on hyphal growth, conidia production and germination. The maize seeds were soaked with fermentation broth to determine the effects of strain KC 121 on maize seed germination. Finally, the bio-control effect of strain KC 121 against Fusarium and the plant-growth promotion effect on maize seedlings were studied by pot experiment. The results showed that after co-culture with KC 121, the hyphae of Fusarium were wrinkled and sunken, and conidia production and germination were also inhibited. The fermentation broth of strain KC 121 could promote the growth of taproot by 61% and bud growth by 162% during maize seed germination. In the pot experiment, the inhibition effects of KC 121 fermentation broth on root rot caused by Fusarium solani and Fusarium sporotrichioides were 66.67% and 76.00%, respectively, and the dry and fresh weight of maize were increased by 130% and 90%, respectively. The KC 121 strain promoted the growth of maize seedlings by producing amylase, producing 6.19 ng/ml indole acetic acid (IAA) and increasing the total chlorophyll content. The B. tequilensis KC 121 showed important potential in controlling the Fusarium disease of maize and promoting maize growth.

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备注/Memo

备注/Memo:
收稿日期：2023-01-12基金项目：国家自然科学基金项目（31660002）；云南省地方本科高校（部分）基础研究联合专项（2018FH001-003）；省部共建云南生物资源保护与利用国家重点实验室开放课题（2019KF005）；云南省“万人计划”青年拔尖人才资助项目(YNWR-QNBJ-2018-011)；云南省教育厅科学研究基金项目（2021Y733、2022Y715）作者简介：吴雅杰（1998-），女，河北石家庄人，硕士研究生，主要从事农业微生物研究。（E-mail）1143106262@qq.com通讯作者：曹艳茹，（E-mail） yanrucao3@aliyun.com
更新日期/Last Update: 2024-03-17