[1]詹丽,李敬丹,付璇,等.紫茉莉种子中对草地贪夜蛾的杀虫活性成分及杀虫机制[J].江苏农业学报,2024,(01):47-54.[doi:doi:10.3969/j.issn.1000-4440.2024.01.005]
　ZHAN Li,LI Jing-dan,FU Xuan,et al.Insecticidal active ingredients and mechanism against Spodoptera frugiperda in Mirabilis jalapa seeds[J].,2024,(01):47-54.[doi:doi:10.3969/j.issn.1000-4440.2024.01.005]
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紫茉莉种子中对草地贪夜蛾的杀虫活性成分及杀虫机制()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2024年01期
页码:
47-54
栏目:
植物保护
出版日期:
2024-01-30

文章信息/Info

Title:
Insecticidal active ingredients and mechanism against Spodoptera frugiperda in Mirabilis jalapa seeds
作者:
詹丽1; 李敬丹1; 付璇1; 梁宗锁2; 梁倩1
(1.西南林业大学林学院,云南昆明650224；2.浙江理工大学绍兴生物医药研究院,浙江绍兴312000)
Author(s):
ZHAN Li1; LI Jing-dan1; FU Xuan1; LIANG Zong-suo2; LIANG Qian1
(1.College of Forestry, Southwest Forestry University, Kunming 650224, China；2.Shaoxing Academy of Biomedicine, Zhejiang Sci-Tech University, Shaoxing 312000, China)
关键词:
紫茉莉种子; 草地贪夜蛾; 杀虫活性; 亚油酸乙酯
Keywords:
Mirabilis jalapa seeds; Spodoptera frugiperda; insecticidal activity; ethyl linoleate
分类号:
S433.4
DOI:
doi:10.3969/j.issn.1000-4440.2024.01.005
文献标志码:
A
摘要:
为了研究紫茉莉种子中对草地贪夜蛾的杀虫活性成分,对紫茉莉种子石油醚萃取物进行硅胶柱层析分离和气相色谱-质谱法(GC-MS)分析,采用浸虫法测定各馏分及化学成分对草地贪夜蛾的杀虫活性。结果表明,采用硅胶柱层析法分离紫茉莉种子石油醚萃取物,共得到20个馏分,其中馏分2对草地贪夜蛾3龄幼虫的校正死亡率最高,第7 d时为82.00%。经GC-MS分析,馏分2的主要化学成分为十三烷(9.59%)、亚油酸乙酯(51.14%)和油酸乙酯(34.26%)。这3种化学成分对草地贪夜蛾3龄幼虫处理后第7 d,LC50分别为15.06 mg/ml、5.70 mg/ml和18.42 mg/ml,亚油酸乙酯的杀虫活性最好。随亚油酸乙酯质量浓度的增加,乙酰胆碱酯酶(AChE)活性呈现先抑制后激活再抑制的趋势,羧酸酯酶(CarE)活性呈现先抑制后激活的趋势,Ca2+-Mg2+-ATP酶活性呈现先激活后抑制的趋势。综上,紫茉莉种子石油醚萃取物中杀虫活性最好的化合物为亚油酸乙酯,可为新型植物源杀虫剂的开发利用提供理论依据。
Abstract:
In order to study the insecticidal active components of Mirabilis jalapa seeds against Spodoptera frugiperda, the petroleum ether extracts from Mirabilis jalapa seeds were separated by silica gel column chromatography and analyzed by gas chromatography-mass spectrometry (GC-MS). The insecticidal activity of each fraction and chemical composition against Spodoptera frugiperda was determined by insect immersion method. The results showed that the petroleum ether extract was separated by silica gel column chromatography, and a total of 20 fractions were obtained. Fraction 2 displayed the highest corrected mortality rate for the 3rd instar larvae of Spodoptera frugiperda, reaching 82.00% on the 7th day. According to GC-MS analysis, the main chemical components of fraction 2 were tridecane (9.59%), ethyl linoleate (51.14%), and ethyl oleate (34.26%). On the 7th day, the LC50 values of the three chemical components against the 3rd instar larvae of Spodoptera frugiperda were 15.06 mg/ml, 5.70 mg/ml, and 18.42 mg/ml, respectively. Ethyl linoleate had the best insecticidal activity. With the increase of ethyl linoleate concentration, the activity of acetylcholinesterase (AChE) showed a trend of first inhibition, then activation, and then inhibition, and the activity of carboxylesterase (CarE) revealed a trend of first inhibition and then activation, while the activity of Ca2+-Mg2+-ATPase indicated a trend of first activation and then inhibition. In summary, ethyl linoleate was the compound with the best insecticidal activity in the petroleum ether extract of Mirabilis jalapa seeds, which could provide theoretical basis for the development and utilization of new plant insecticides.

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备注/Memo

备注/Memo:
收稿日期：2023-10-24基金项目：浙江省科技计划项目(2022C02023)；国家自然科学基金青年基金项目(31201572)；西南林业大学人才引进项目(111307)作者简介：詹丽(1998-),女,贵州毕节人,硕士研究生,研究方向为植物多样性保护与利用。(E-mail)2859202094@qq.com通讯作者：梁倩,(E-mail)liangqian533@163.com
更新日期/Last Update: 2024-03-17