[1]涂远璐,汤海江,张凯,等.过瘤胃胆碱和过瘤胃葡萄糖添加对围产期母羊能量负平衡的影响[J].江苏农业学报,2023,(07):1567-1574.[doi:doi:10.3969/j.issn.1000-4440.2023.07.013]
　TU Yuan-lu,TANG Hai-jiang,ZHANG Kai,et al.Effects of rumen-protected choline and rumen-protected glucose supplementation on negative energy balance of perinatal ewes[J].,2023,(07):1567-1574.[doi:doi:10.3969/j.issn.1000-4440.2023.07.013]
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过瘤胃胆碱和过瘤胃葡萄糖添加对围产期母羊能量负平衡的影响()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2023年07期
页码:
1567-1574
栏目:
畜牧兽医·水产养殖
出版日期:
2023-10-31

文章信息/Info

Title:
Effects of rumen-protected choline and rumen-protected glucose supplementation on negative energy balance of perinatal ewes
作者:
涂远璐1; 2; 汤海江3; 张凯1; 2; 贾学敬1; 白云峰1; 2; 洪伟4
(1.江苏省农业科学院畜牧研究所，江苏南京210014；2.农业农村部种养结合重点实验室，江苏南京210014；3.邳州市小河科技发展有限公司，江苏邳州221300；4.上海美农生物科技股份有限公司，上海200157)
Author(s):
TU Yuan-lu1; 2; TANG Hai-jiang3; ZHANG Kai1; 2; JIA Xue-jing1; BAI Yun-feng1; 2; HONG Wei4
(1.Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;2.Ministry of Agriculture and Rural Affairs, Key Laboratory of Crop and Livestock Integrated Farming, Nanjing 210014, China;3.Pizhou Xiaohe Science and Technology Development Co., Ltd., Pizhou 221300, China;4.Shanghai Menon Animal Nutrition Technology Co.,Ltd., Shanghai 200157, China)
关键词:
过瘤胃胆碱; 过瘤胃葡萄糖; 能量负平衡; 粪便微生物; 湖羊
Keywords:
rumen-protected choline; rumen-protected glucose; negative energy balance; fecal microorganism; Hu sheep
分类号:
S858.26
DOI:
doi:10.3969/j.issn.1000-4440.2023.07.013
文献标志码:
A
摘要:
本文通过考察血酮(血液中酮体)浓度、产羔情况及粪便微生物区系组成，评估过瘤胃胆碱(RPC)和过瘤胃葡萄糖(RPG)缓解围产期母羊能量负平衡的效果。试验期共57 d，从产前30 d到产后20 d，预饲期7 d。选取80只妊娠期(120～130 d)湖羊，随机分为4组，对照组饲喂基础饲粮，RPC组每只每天添加3 g RPC，RPG组添加15 g RPG，RPC+RPG组添加3 g RPC和15 g RPG。结果表明，1)RPG组母羊在产羔当天血酮浓度显著低于对照组(P<0.05)；2)RPC+RPG组羔羊初生质量显著高于其他组(P<0.05)；3)RPC和RPG的添加没有改变门水平和属水平上母羊粪便的优势菌种类，但影响了核心菌群的相对丰度，各试验组厚壁菌门和瘤胃球菌科UCG-010相对丰度均显著低于对照组(P<0.05)，RPC+RPG组拟杆菌门相对丰度显著高于其他组(P<0.05)，RPG组毛螺菌科AC2044群相对丰度显著高于对照组(P<0.05)。由此得出，围产期母羊饲粮中添加RPC和RPG均有助于提高羔羊初生质量，提高母羊肠道中碳水化合物利用菌拟杆菌门的相对丰度，降低纤维分解菌群厚壁菌门的相对丰度，RPG的添加还使得肠道抗炎有益菌毛螺菌科属相对丰度得到提高，同时有效降低血酮浓度。综合考虑，RPG单独添加效果最佳，RPC的适宜添加量有待进一步研究。
Abstract:
In this article, the effects of rumen-protected choline (RPC) and rumen-protected glucose (RPG) on alleviating negative energy balance in periparturient ewes were evaluted by examining blood ketone concentration, lambing performance and fecal microbial composition. The experiment lasted for 57 days, from 30 days before parturition to 20 days after parturition and included a seven-day pre-feeding period. Eighty pregnant (120-130 days) Hu sheep were randomly divided into four groups, the control group fed with basic feed, the RPC group added with 3 g/head RPC per day, the RPG group added with 15 g/head RPG per day, and the RPC+RPG group added with 3 g/head RPC per day plus 15 g/head RPG per day. The results showed that, on the day of lambing, blood ketone concentration of the ewes in the RPG group was significantly lower than that in the control group (P<0.05). The birth weight of lambs in the RPC+RPG group was significantly higher than other groups (P<0.05). The dominant bacterial species at phylum and genus levels in ewe feces did not change with the addition of RPC and RPG, but the relative abundance of core bacterial groups was affected. The relative abundances of Firmicutes and Ruminococcaceae UCG-010 in all experimental groups were significantly lower than that in the control group (P<0.05), and the relative abundance of Bacteroidetes in the RPC+RPG group was significantly higher than that in other groups (P<0.05). The relative abundance of Lachnospiraceae AC2044 group in RPG group was significantly higher than that in the control group (P<0.05). In conclusion, the birth weight of lambs and the relative abundance of carbohydrate-utilizing bacterial group Bacteroidetes of the ewe intestines can be increased by adding RPC or RPG into the feed of periparturient ewes, while the relative abundance of fiber-degrading bacterial group Firmicutes can be reduced. Besides, the relative abundance of beneficial anti-inflammatory bacteria genus of Lachnosiraceae in the ewe intestine can also be increased by adding RPG into the feed of periparturient ewes, while the blood ketone concentration can be reduced effectively. In conclusion, single addition of RPG shows the best effect, and the appropriate addition amount of RPC needs further study.

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备注/Memo

备注/Memo:
收稿日期：2022-09-22基金项目：“十三五”国家重点研发计划项目(2019YFE0125600)作者简介：涂远璐(1983-)，女，江西南昌人，硕士，助理研究员，从事家畜营养与生态研究。(Tel)13851804073;(E-mail)121663004@qq.com通讯作者：白云峰，(Tel)13951009162;(E-mail)Blinkeye@126.com
更新日期/Last Update: 2023-11-17