[1]白茂军,高正锋,张力元,等.烟草青枯病发病程度与土壤环境间的响应关系[J].江苏农业学报,2023,(06):1294-1302.[doi:doi:10.3969/j.issn.1000-4440.2023.06.004]
　BAI Mao-jun,GAO Zheng-feng,ZHANG Li-yuan,et al.The relationship between the incidence of bacterial wilt disease and soil environment in tobacco[J].,2023,(06):1294-1302.[doi:doi:10.3969/j.issn.1000-4440.2023.06.004]
点击复制

烟草青枯病发病程度与土壤环境间的响应关系()
分享到：

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2023年06期
页码:
1294-1302
栏目:
植物保护
出版日期:
2023-09-30

文章信息/Info

Title:
The relationship between the incidence of bacterial wilt disease and soil environment in tobacco
作者:
白茂军1; 高正锋2; 张力元3; 范成平1; 潘首慧1; 董延鑫1; 杨索1; 王莹1
(1.贵州省烟草公司安顺市公司，贵州安顺561000；2.云南农业大学，云南昆明650201；3.贵州中烟工业有限责任公司，贵州贵阳550009)
Author(s):
BAI Mao-jun1; GAO Zheng-feng2; ZHANG Li-yuan3; FAN Cheng-ping1; PAN Shou-hui1; DONG Yan-xin1; YANG Suo1; WANG Ying1
(1.Anshun Branch of Guizhou Tobacco Company, Anshun 561000, China;2.Yunnan Agricultural University, Kunming 650201, China;3.China Tobacco Guizhou Industrial Co., Ltd., Guiyang 550009, China)
关键词:
烟草青枯病; 土壤理化性状; 酶活性; 细菌; 真菌
Keywords:
tobacco bacterial wilt; soil physical and chemical properties; enzyme activity; bacteria; fungi
分类号:
S435.72
DOI:
doi:10.3969/j.issn.1000-4440.2023.06.004
文献标志码:
A
摘要:
为探究烟草青枯病发生与土壤生态环境因子间的关系，明确与青枯病发病程度相关的因素，通过田间调查收集青枯病不同发病程度的根际土壤，测定土壤理化指标和酶活性并用16S DNA、内转录间隔区(Internally transcribed spacer，ITS)基因测序技术分析烟株发病与未发病植株根际土壤细菌、真菌群落结构的差异。结果表明，在烟株发生青枯病的根际土壤中，随着烟株发病程度的加重，土壤pH值、有机质含量、总氮含量降低，硝态氮(NO-3-N)含量升高；发病烟株根际土壤真菌群落中镰刀菌属(Fusarium)、毛霉属(Mucor)、Pseudaleuria与细菌群落中肠杆菌属(Enterobacter)、鞘氨醇单胞菌属(Sphingomonas)的相对丰度高于正常烟株根际土壤；通过LEFse及相关性分析发现，柱孢霉菌属(Cylindrocarpon)、毛霉属(Mucor)、鞘氨醇单胞菌属(Sphingomonas)可能是不同青枯病发病程度烟株根际土壤中微生物在属水平产生差异的主要物种；冗余分析(RDA)结果表明，总氮含量、有机质含量可能是影响烟草青枯病发生的关键土壤因子。综上所述，土壤总氮含量、有机质含量与鞘氨醇单胞菌属(Sphingomonas)、毛霉属(Mucor)微生物相对丰度的降低以及柱孢霉菌属微生物相对丰度的增加是引起烟草青枯病严重发生的关键因素。
Abstract:
In order to explore the relationship between the occurrence of tobacco wilt and soil ecological and environmental factors, and to clarify the factors related to the incidence of wilt, rhizosphere soil with different degrees of wilt was collected through field investigation, soil physicochemical indices and enzyme activities were measured, and the differences of bacterial and fungal community structure in rhizosphere soil of pathogenic and non-diseased tobacco plants were analyzed by 16S DNA and internally transcribed spacer (ITS) gene sequencing technology. The results showed that in the rhizosphere soil of tobacco plants with bacterial wilt, with the aggravation of the degree of disease, the soil pH value, organic matter content and total nitrogen content decreased, and the content of nitrate nitrogen (NO-3-N) increased. The relative abundance of Fusarium, Mucor and Pseudaleuria in the fungal community and Enterobacter and Sphingomonas in the bacterial community in the rhizosphere soil of diseased tobacco plants was higher than that in the rhizosphere soil of normal tobacco plants. Through LEFse and correlation analysis, it was found that Cylindrocarpon, Mucor, and Sphingomonas may be the main different species of microorganisms in the rhizosphere soil of tobacco plants with different degrees of wilt at the genus level. The results of redundancy analysis (RDA) showed that total nitrogen content and organic matter content may be the key soil factors affecting the occurrence of wilt. In summary, the decrease of soil total nitrogen content, organic matter content and relative abundance of Sphingomonas and Mucor, and the increase of relative abundance of Cylindrocarpon were the key factors causing the serious occurrence of wilt.

参考文献/References:

[1]吴永铭,钟小丽,谢凤标. 土壤调理剂对植烟土壤理化性状及烤烟产质量的影响[J].现代农业科技, 2021(20):6-9.
[2]章文水,张瀛,王雪仁,等. 不同土壤改良措施对植烟土壤理化性状及烟草青枯病的影响[J].中国烟草科学, 2019,40(2):16-22.
[3]王垚,韩松庭,杨亮,等. 生物有机肥对烟草青枯病防控的研究进展[J].植物医生, 2020,33(6):18-23.
[4]刘勇,秦西云,李文正,等. 抗青枯病烟草种质资源在云南省的评价[J].植物遗传资源学报,2010,11(1):10-16.
[5]单晓鹏. 烟草青枯病的防治研究进展[J].现代农业科技, 2021(11):111-113.
[6]叶建如,顾钢,张瀛. 几种药剂防治烟草青枯病的药效评价[J].中国烟草科学,2013,34(1):72-76.
[7]刘艳霞,李想,曹毅,等. 抑制烟草青枯病型生物有机肥的田间防效研究[J].植物营养与肥料学报,2014,20(5):1203-1211.
[8]张永春,黄镇,关国经,等. 不同农业生态调控措施对烟草青枯病的影响[J].中国烟草科学,2007(4):49-52.
[9]方树民,唐莉娜,陈顺辉,等. 作物轮作对土壤中烟草青枯菌数量及发病的影响[J].中国生态农业学报,2011,19(2):377-382.
[10]孙思,王军. 青枯病发病率与土壤条件关系的研究进展[J].江西植保, 2005(1):17-20.
[11]倪纪恒. 不同土壤类型与调控措施对烟草根系生长与分布的影响[D].郑州:河南农业大学,2002.
[12]匡传富,何志明,汤若云,等. 烟草青枯病土壤微生物数量及生理群的测定[J].中国烟草科学, 2003(1):43-45.
[13]章文水,张瀛,王雪仁,等. 不同土壤改良措施对植烟土壤理化性状及烟草青枯病的影响[J].中国烟草科学, 2019,40(2):16-22.
[14]樊俊,谭军,王瑞,等. 烟草青枯病发病程度的影响因素分析[J].烟草科技, 2021,54(10):20-28.
[15]吴晓宗. 烟草青枯病发病对植烟根际土壤微生态的影响[D].郑州:郑州大学,2020.
[16]黎妍妍,王林,彭五星,等. 烟草青枯病不同发病阶段根际土壤微生物群落变化趋势分析[J].中国烟草科学,2020,41(5):73-78.
[17]高游慧, 郑泽慧, 张越,等. 根际微生态防治作物土传真菌病害的机制研究进展[J].中国农业大学学报, 2021, 26(6):100-113.
[18] 国家烟草专卖局.烟草病虫害分级及调查方法:GB/T 23222-2008[S].北京:中华人民共和国国家质量监督检验检疫总局，中国国家标准化管理委员会，2008.
[19]番华彩,唐嘉义,秦小萍. 烟草青枯病防治研究进展[J].云南大学学报(自然科学版), 2008，30(增刊1):31-35.
[20]李红丽,李清飞,郭夏丽,等. 调节土壤微生态防治烟草青枯病[J].河南农业科学,2006(2):57-60.
[21]汪汉成,余婧,蔡刘体,等. 温度、湿度、接菌量及pH对烟草青枯病菌致病力的影响[J].中国烟草科学,2017,38(5):8-12.
[22]BUDAKOV D, NAGL N, STOJIN V, et al. Sensitivity of Cercospora beticola isolates from Serbia to carbendazim and flutriafol[J]. Crop Protection, 2014, 66: 120-126.
[23]何万泽. 烟草不同氮源水平对赤星病的抗性研究[J].烟草科技, 2001(3):45-46.
[24]赵芳,赵正雄,徐发华,等. 施氮量对烟株接种黑胫病前、后体内生理物质及黑胫病发生的影响[J].植物营养与肥料学报,2011,17(3):737-743.
[25]谭军,周冀衡,李强,等. 氮素形态对烟草黑胫病发生的影响[J].中国烟草科学,2017,38(4):80-85.
[26]董鲜,郑青松,王敏,等. 铵态氮和硝态氮对香蕉枯萎病发生的比较研究[J].植物病理学报,2015,45(1):73-79.
[27]郭莹,杨晓云,司朝光,等. 不同形态氮素营养对大白菜芝麻状斑点病发生的影响[J].园艺学报,2011,38(8):1489-1497.
[28]史普酉,杨成翠,贾孟,等. 不同黑胫病发病程度下植烟根际土壤酶活性及细菌群落结构差异比较[J].中国土壤与肥料,2020(1):179-187.
[29]寻路路,赵宏光,梁宗锁,等. 三七根腐病病株和健株根域土壤微生态研究[J].西北农业学报,2013,22(11):146-151.
[30]廖梓良,孙世中,刘建香,等. 设施栽培香石竹根际土壤酶活与土传病害相关性研究[J].云南师范大学学报(自然科学版),2009,29(3):59-63.
[31]宋帅,赵艳艳,刘亦晗,等.轮作大蒜对番茄植株根际土壤微生态的影响[J].北方园艺,2022(23):98-105.
[32]LI Y L, DAI S Y, WANG B Y, et al. Autotoxic ginsenoside disrupts soil fungal microbiomes by stimulating potentially pathogenic microbes[J].Applied and Environmental Microbiology,2020,86(9):e00130-20.
[33]高芬,岳换弟,秦雪梅,等. 植物致病镰刀菌细胞壁降解酶的研究进展[J].江苏农业学报,2018,34(4):955-960.
[34]张天宇,李恭民,陈伟群,等. 西洋参锈腐病病原研究[J].西北农林科技大学学报(自然科学版),1991(1):43-48.
[35]HILDEBRAND A A. Root rot of ginseng in Ontario caused by member of the genus Rumularia[J].Canadian Journal of Research,2011,12(1):82-114.
[36]刘雨艳,保丽美,魏云林,等.连作三七根际土壤自毒物质与微生物互作研究进展[J].中药材,2022,45(1):242-247.
[37]LEE S M, KONG H G, SONG G C, et al. Disruption of Firmicutes and Actinobacteria abundance in tomato rhizosphere causes the incidence of bacterial wilt disease[J]. The ISME Journal, 2021, 15(1):330-347.
[38]黄芳芳. 农药对三七根际土壤被孢霉的影响及其田间定殖能力[D].昆明:云南大学,2017.
[39]黎妍妍,王林,彭五星,等.烟草青枯病不同发病阶段根际土壤微生物群落变化趋势分析[J].中国烟草科学,2020,41(5):73-78.

相似文献/References:

[1]李春宏,殷剑美,王立,等.连作对芋头根际土壤理化性状和微生物特性的影响[J].江苏农业学报,2019,(04):825.[doi:doi:10.3969/j.issn.1000-4440.2019.04.011]
　LI Chun hong,YIN Jian mei,WANG Li,et al.Effects of continuous cropping on soil physicochemical and microbial properties of taro[J].,2019,(06):825.[doi:doi:10.3969/j.issn.1000-4440.2019.04.011]
[2]高富成,李海俊,穆晓国,等.化肥减量处理下蚯蚓-牛粪协同作用对菜薹生长及土壤中重金属锌、砷和铬含量的影响[J].江苏农业学报,2024,(06):1078.[doi:doi:10.3969/j.issn.1000-4440.2024.06.015]
　GAO Fucheng,LI Haijun,MU Xiaoguo,et al.Synergistic effects of earthworms and cow manure on the growth of flowering Chinese cabbage and contents of heavy metals zinc, arsenic and chromium in soil under reduced chemical fertilization[J].,2024,(06):1078.[doi:doi:10.3969/j.issn.1000-4440.2024.06.015]
[3]李世金,林硕,马称心,等.生物质灰加生物菌剂对烟草根际土壤微生物群落的影响以及对青枯病的防治效果[J].江苏农业学报,2024,(12):2237.[doi:doi:10.3969/j.issn.1000-4440.2024.12.006]
　LI Shijin,LIN Shuo,MA Chenxin,et al.Effects of combined application of biomass ash and bio-bacterial agents on the microbial community in tobacco rhizosphere soil and the control effect on bacterial wilt[J].,2024,(06):2237.[doi:doi:10.3969/j.issn.1000-4440.2024.12.006]

备注/Memo

备注/Memo:
收稿日期:2022-08-23基金项目:贵州省烟草公司重点研发项目(2021XM15)；云南省教育厅科学研究基金项目(2022Y250)作者简介:白茂军(1983-)，男，重庆人，助理农艺师，农业推广硕士，从事烤烟种植与收购工作。(E-mail)772390297@qq.com通讯作者:张力元，(E-mail) 760061799@qq.com
更新日期/Last Update: 2023-11-17