[1]白茂军,高正锋,张力元,等.烟草青枯病发病程度与土壤环境间的响应关系[J].江苏农业学报,2023,(06):1294-1302.[doi:doi:10.3969/j.issn.1000-4440.2023.06.004]
　BAI Mao-jun,GAO Zheng-feng,ZHANG Li-yuan,et al.The relationship between the incidence of bacterial wilt disease and soil environment in tobacco[J].,2023,(06):1294-1302.[doi:doi:10.3969/j.issn.1000-4440.2023.06.004]
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烟草青枯病发病程度与土壤环境间的响应关系()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2023年06期
页码:
1294-1302
栏目:
植物保护
出版日期:
2023-09-30

文章信息/Info

Title:
The relationship between the incidence of bacterial wilt disease and soil environment in tobacco
作者:
白茂军1; 高正锋2; 张力元3; 范成平1; 潘首慧1; 董延鑫1; 杨索1; 王莹1
(1.贵州省烟草公司安顺市公司，贵州安顺561000；2.云南农业大学，云南昆明650201；3.贵州中烟工业有限责任公司，贵州贵阳550009)
Author(s):
BAI Mao-jun1; GAO Zheng-feng2; ZHANG Li-yuan3; FAN Cheng-ping1; PAN Shou-hui1; DONG Yan-xin1; YANG Suo1; WANG Ying1
(1.Anshun Branch of Guizhou Tobacco Company, Anshun 561000, China;2.Yunnan Agricultural University, Kunming 650201, China;3.China Tobacco Guizhou Industrial Co., Ltd., Guiyang 550009, China)
关键词:
烟草青枯病; 土壤理化性状; 酶活性; 细菌; 真菌
Keywords:
tobacco bacterial wilt; soil physical and chemical properties; enzyme activity; bacteria; fungi
分类号:
S435.72
DOI:
doi:10.3969/j.issn.1000-4440.2023.06.004
文献标志码:
A
摘要:
为探究烟草青枯病发生与土壤生态环境因子间的关系，明确与青枯病发病程度相关的因素，通过田间调查收集青枯病不同发病程度的根际土壤，测定土壤理化指标和酶活性并用16S DNA、内转录间隔区(Internally transcribed spacer，ITS)基因测序技术分析烟株发病与未发病植株根际土壤细菌、真菌群落结构的差异。结果表明，在烟株发生青枯病的根际土壤中，随着烟株发病程度的加重，土壤pH值、有机质含量、总氮含量降低，硝态氮(NO-3-N)含量升高；发病烟株根际土壤真菌群落中镰刀菌属(Fusarium)、毛霉属(Mucor)、Pseudaleuria与细菌群落中肠杆菌属(Enterobacter)、鞘氨醇单胞菌属(Sphingomonas)的相对丰度高于正常烟株根际土壤；通过LEFse及相关性分析发现，柱孢霉菌属(Cylindrocarpon)、毛霉属(Mucor)、鞘氨醇单胞菌属(Sphingomonas)可能是不同青枯病发病程度烟株根际土壤中微生物在属水平产生差异的主要物种；冗余分析(RDA)结果表明，总氮含量、有机质含量可能是影响烟草青枯病发生的关键土壤因子。综上所述，土壤总氮含量、有机质含量与鞘氨醇单胞菌属(Sphingomonas)、毛霉属(Mucor)微生物相对丰度的降低以及柱孢霉菌属微生物相对丰度的增加是引起烟草青枯病严重发生的关键因素。
Abstract:
In order to explore the relationship between the occurrence of tobacco wilt and soil ecological and environmental factors, and to clarify the factors related to the incidence of wilt, rhizosphere soil with different degrees of wilt was collected through field investigation, soil physicochemical indices and enzyme activities were measured, and the differences of bacterial and fungal community structure in rhizosphere soil of pathogenic and non-diseased tobacco plants were analyzed by 16S DNA and internally transcribed spacer (ITS) gene sequencing technology. The results showed that in the rhizosphere soil of tobacco plants with bacterial wilt, with the aggravation of the degree of disease, the soil pH value, organic matter content and total nitrogen content decreased, and the content of nitrate nitrogen (NO-3-N) increased. The relative abundance of Fusarium, Mucor and Pseudaleuria in the fungal community and Enterobacter and Sphingomonas in the bacterial community in the rhizosphere soil of diseased tobacco plants was higher than that in the rhizosphere soil of normal tobacco plants. Through LEFse and correlation analysis, it was found that Cylindrocarpon, Mucor, and Sphingomonas may be the main different species of microorganisms in the rhizosphere soil of tobacco plants with different degrees of wilt at the genus level. The results of redundancy analysis (RDA) showed that total nitrogen content and organic matter content may be the key soil factors affecting the occurrence of wilt. In summary, the decrease of soil total nitrogen content, organic matter content and relative abundance of Sphingomonas and Mucor, and the increase of relative abundance of Cylindrocarpon were the key factors causing the serious occurrence of wilt.

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备注/Memo

备注/Memo:
收稿日期:2022-08-23基金项目:贵州省烟草公司重点研发项目(2021XM15)；云南省教育厅科学研究基金项目(2022Y250)作者简介:白茂军(1983-)，男，重庆人，助理农艺师，农业推广硕士，从事烤烟种植与收购工作。(E-mail)772390297@qq.com通讯作者:张力元，(E-mail) 760061799@qq.com
更新日期/Last Update: 2023-11-17