[1]刘志刚,余方伟,张伟,等.甘蓝黑斑病病原菌鉴定及其对杀菌剂的敏感性[J].江苏农业学报,2023,(04):947-955.[doi:doi:10.3969/j.issn.1000-4440.2023.04.004]
　LIU Zhi-gang,YU Fang-wei,ZHANG Wei,et al.Identification and fungicide sensitivity of the pathogen causing black spot on Brassica oleracea var. capitata L.[J].,2023,(04):947-955.[doi:doi:10.3969/j.issn.1000-4440.2023.04.004]
点击复制

甘蓝黑斑病病原菌鉴定及其对杀菌剂的敏感性()
分享到：

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2023年04期
页码:
947-955
栏目:
植物保护
出版日期:
2023-08-30

文章信息/Info

Title:
Identification and fungicide sensitivity of the pathogen causing black spot on Brassica oleracea var. capitata L.
作者:
刘志刚1; 余方伟2; 张伟2; 于利2; 李建斌2; 王神云2; 宋江华1
(1.安徽农业大学园艺学院，安徽合肥230036；2.江苏省农业科学院蔬菜研究所，江苏南京210014)
Author(s):
LIU Zhi-gang1; YU Fang-wei2; ZHANG Wei2; YU Li2; LI Jian-bin2; WANG Shen-yun2; SONG Jiang-hua1
（1.College of Horticulture, Anhui Agricultural University, Hefei 230036, China；2.Institute of Vegetable Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China）
关键词:
甘蓝; 黑斑病; 芸薹生链格孢菌; 杀菌剂
Keywords:
Brassica oleracea var. capitata L.; black spot; Alternaria brassicicola; fungicide
分类号:
S436.35
DOI:
doi:10.3969/j.issn.1000-4440.2023.04.004
文献标志码:
A
摘要:
对江苏省甘蓝（Brassica oleracea var. capitata L.）叶片上发生的黑斑病进行病原菌鉴定，并测试病原菌对杀菌剂的敏感性。经形态学观察，初步明确分离得到的病原菌（HB0、HB1、HB2、HB3、HB5、HB6）为链格孢属真菌。基于科赫氏法则测定病原菌致病性，发现HB1、HB3、HB6能在甘蓝叶片上致病。扩增上述3个致病菌株的核糖体DNA内转录间隔区（rDNA-ITS）、过敏原基因（Alt a1）、3-磷酸甘油醛脱氢酶基因（GAPDH），得到大小为511～596 bp目的片段。多基因联合聚类分析结果显示，引起江苏省甘蓝黑斑病的病原菌为芸薹生链格孢（Alternaria brassicicola）。室内毒力试验发现，在测试的8种杀菌剂中，苯醚甲环唑、咯菌腈、己唑醇、氟硅唑、戊唑醇、丙环唑、异菌脲对病原菌菌丝生长的抑制效果较好，代森锰锌对病原菌菌丝生长的抑制作用较弱。
Abstract:
The pathogen was isolated from cabbage (Brassica oleracea var. capitata L. ) leaves showing black spot symptom in Jiangsu province, and its sensitivity to fungicides was tested. Morphology observation showed that the isolated fungi (HB0, HB1, HB2, HB3, HB6, HB6) belonged to Alternaria spp. The pathogenicity of the isolated pathogens was determined according to Koch’s postulates. HB1, HB3 and HB6 were able to cause disease on cabbage leaves. Then the internal transcribed spacer of ribosomal DNA (rDNA-ITS), allergen gene (Alt a1) and glyceraldehyde-3-phosphate dehydrogenase gene (GAPDH) were amplified from HB1, HB3 and HB6, and the target fragments with a length of 511-596 bp were obtained. The results of multi-gene phylogenetic analysis revealed that the pathogen causing black spot on cabbage in Jiangsu province was Alternaria brassicicola. Furthermore, among eight tested fungicides, difenoconazole, fludioxonil, hexaconazole, flusilazole, tebuconazole, propiconazole and iprodione had stronger inhibitory effects against the mycelial growth of pathogen, while mancozeb had weaker inhibitory effect.

参考文献/References:

[1]辛竹琳，崔彦娟，杨小薇，等. 全球蔬菜产业现状及中国蔬菜育种发展路径研究进展[J]. 分子植物育种，2022，20(9)：3122-3132.
[2]陈永生，崔志超，杨雅婷，等. 关于甘蓝类蔬菜种植模式规范化的建议[J]. 长江蔬菜，2021(8)：20-21.
[3]SAHARAN G S，MEHTA N，MEENA P D，et al. Alternaria diseases of crucifers: Biology，ecology and disease management[M]. Singapore：Springer，2016.
[4]DHARMENDRA K，NEELAM M，YASHWANT K B，et al. Alternaria blight of oilseed Brassicas: A comprehensive review[J]. African Journal of Microbiology Research，2014，8(30)：2816-2829.
[5]MUKESH M，SWARNMALA S. Alternaria host-specific (HSTs) toxins: An overview of chemical characterization，target sites，regulation and their toxic effects[J]. Toxicology Reports，2019，6：745-758.
[6]SOLFRIZZO M. Recent advances on Alternaria mycotoxins[J]. Current Opinion in Food Science，2017，17：57-61.
[7]SIMMONS E G. Alternaria, an identification manual[M]. Washington: ASM Press，2007.
[8]王超，张晓烜，王宁宁. 甘蓝黑斑病病原菌鉴定[C]//中国园艺学会. 中国园艺学会十字花科蔬菜分会第十届学术研讨会论文集.天津：中国园艺学会，2012：166-170.
[9]方中达. 植病研究方法[M]. 北京：中国农业出版社，1998.
[10]CENIS J L. Rapid extraction of fungal DNA for PCR amplification[J]. Nucleic Acids Research，1992，20(9)：2380.
[11]WHITE T J，BRUNS T，LEE S，et al. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics[J]. PCR Protocols: A Guide to Methods and Applications, 1990，18(1)：315-322.
[12]HONG S G，CRAMER R A，LAWRENCE C B，et al. Alt a1 allergen homologs from Alternaria and related taxa: analysis of phylogenetic content and secondary structure[J]. Fungal Genetics and Biology，2005，42(2)：119-129.
[13]BERBEE M L，PIRSEYEDI M，HUBBARD S. Cochliobolus phylogenetics and the origin of known，highly virulent pathogens，inferred from ITS and glyceraldehyde-3-phosphate dehydrogenase gene sequences[J]. Mycologia，1999，91(6)：964-977.
[14]蔡永占,白涛,刘冬梅,等.烟草赤星病高效生防内生细菌的分离筛选及发酵培养条件优化[J].江苏农业科学,2022,50(13):129-135.
[15]朱杰,程亮,张纲,等.樱桃叶斑病生防菌株萎缩芽孢杆菌菌株QH-588的筛选鉴定[J].南方农业学报,2021,52(11):3022-3033.
[16]娄喜艳,郭洋洋,裴冬丽. 河南商丘月季黑斑病病原菌鉴定及生物学特性[J].江苏农业科学,2021,49(19):138-143.
[17]何洁,梁霜,张国俊,等. 太子参叶斑病病原鉴定及室内药剂筛选[J].南方农业学报,2021,52(8):2124-2132.
[18]WOUDENBERG J H C，SEIDL M F，GEOENEWALD J Z，et al. Alternaria section Alternaria: Species，formae speciales or pathotypes?[J]. Studies in Mycology，2015，82：1-21.
[19]CHENG H，ZHAO L，WEI X，et al. Alternaria species causing leaf spot on hemp (Cannabis sativa) in Northern China[J]. European Journal of Plant Pathology，2022，162：957-970.
[20]WANG B，ZHANG Y Y，LIU J P，et al. A new leaf blight disease caused by Alternaria jacinthicola on banana in China[J]. Horticulturae，2021，8(1）：12.
[21]施兆荣，张广荣，孙述俊，等. 甘肃省甜瓜黑斑病菌（Alternaria tenuissima）的分离鉴定[J]. 园艺学报，2022，49(2)：427-436.
[22]刘焕然，柯桂兰. 十字花科蔬菜黑斑病原种群组成及季节变化[J]. 西北农业学报，1992，1(4)：6-10.
[23]沈钰森，王建升，盛小光，等. 十字花科植物黑斑病的研究进展[J]. 核农学报，2021，35(3)：623-634.
[24]PHAPALE A D，SOLANKY K U，TAYADE S C，et al. Screening of fungicides against okra leaf spot under laboratory condition[J]. International Journal of Plant Protection，2010，3(2)：282-284.
[25]刘霞，杨克强，姜兴印，等. 危害核桃的链格孢(Alternaria alternata)对4种杀菌剂的敏感性[J]. 农药，2013，52(1)：67-70,77.
[26]李亚婷，孙彤彤，罗强，等. 4种杀菌剂防治白菜黑斑病的毒力测定研究[J]. 河北农业大学学报，2018，41(4)：96-100.
[27]包媛媛，赵娟秀，杨加艳，等. 不同杀菌剂对三七黑斑病菌的毒力测定[J]. 中国农学通报，2018，34(2)：74-78.
[28]JACKSON，KABEABAM，KUMAR，et al. Management of Alternaria leaf spot of mustard through chemical and biological agents[J]. Plant Cell Biotechnology and Molecular Biology，2019，20(3)：162-178.
[29]冯宝珍，李培谦. 月季黑斑病病原菌鉴定及室内药剂初步筛选[J]. 植物保护学报，2019，46(5)：1147-1154.
[30]陶航，扎依娜·玛合巴提，张烨，等. 芍药黑斑病病原菌鉴定及其对杀菌剂敏感性分析[J]. 园艺学报，2021，48(1)：173-182.

相似文献/References:

[1]闫圆圆,曾爱松,宋立晓,等.结球甘蓝幼苗耐热性鉴定方法及耐热生理[J].江苏农业学报,2016,(04):885.[doi:10.3969/j.issn.100-4440.2016.04.027]
　YAN Yuan-yuan,ZENG Ai-song,SONG Li-xiao,et al.Identification of heat tolerance in cabbage seedlings and heat-tolerant physiology[J].,2016,(04):885.[doi:10.3969/j.issn.100-4440.2016.04.027]
[2]戴忠良,陈丽,山溪,等.甘蓝晚抽薹基因BoFLC3克隆、序列分析和亚细胞定位[J].江苏农业学报,2018,(06):1324.[doi:doi:10.3969/j.issn.1000-4440.2018.06.018]
　DAI Zhong-liang,CHEN Li,SHAN Xi,et al.Cloning, sequence analysis and subcellular localization of Brassica oleraceaBoFLC3 gene[J].,2018,(04):1324.[doi:doi:10.3969/j.issn.1000-4440.2018.06.018]
[3]秦文斌,戴忠良,山溪,等.甘蓝冷胁迫相关基因BobHLH18克隆与表达分析[J].江苏农业学报,2019,(01):149.[doi:doi:10.3969/j.issn.1000-4440.2019.01.022]
　QIN Wen-bin,DAI Zhong-liang,SHAN Xi,et al.Molecular cloning and expression analysis of cold stress-related gene BobHLH18 in cabbage (Brassica oleracea var. capitata L.)[J].,2019,(04):149.[doi:doi:10.3969/j.issn.1000-4440.2019.01.022]
[4]郭峰,孙莹,安容慧,等.LED绿光处理对甘蓝采后品质及抗氧化活性的影响[J].江苏农业学报,2023,(02):489.[doi:doi:10.3969/j.issn.1000-4440.2023.02.022]
　GUO Feng,SUN Ying,AN Rong-hui,et al.Effects of green light-emitting diode light treatment on the quality and antioxidant capacity of postharvest green cabbage (Brassica oleracea var. capitata L.)[J].,2023,(04):489.[doi:doi:10.3969/j.issn.1000-4440.2023.02.022]

备注/Memo

备注/Memo:
收稿日期：2022-08-16 基金项目：江苏省重点研发计划（现代农业）项目（BE2021376）；国家现代农业产业技术体系建设专项资金项目（CARS-23-G42）；国家自然科学基金项目（32272728）作者简介：刘志刚（1998-），男，安徽合肥人，硕士研究生，主要从事甘蓝病害鉴定及种质资源筛选利用研究。（E-mail）lzgang@stu.ahau.edu.cn 通讯作者：宋江华，（E-mail）jhsong@ahau.edu.cn；王神云，（E-mail）wangshenyun@jaas.ac.cn
更新日期/Last Update: 2023-09-12