[1]原佳妮,赵延辉,侍玉梅,等.利用WGCNA挖掘种公鸡睾丸和附睾中影响精子活力的核心基因[J].江苏农业学报,2023,(03):762-769.[doi:doi:10.3969/j.issn.1000-4440.2023.03.017]
　YUAN Jia-ni,ZHAO Yan-hui,SHI Yu-mei,et al.Mining of hub genes affecting sperm motility in testes and epididymides of breeder cocks by WGCNA method[J].,2023,(03):762-769.[doi:doi:10.3969/j.issn.1000-4440.2023.03.017]
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利用WGCNA挖掘种公鸡睾丸和附睾中影响精子活力的核心基因()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2023年03期
页码:
762-769
栏目:
畜牧兽医·水产养殖
出版日期:
2023-06-30

文章信息/Info

Title:
Mining of hub genes affecting sperm motility in testes and epididymides of breeder cocks by WGCNA method
作者:
原佳妮; 赵延辉; 侍玉梅; 倪和民; 郭勇; 盛熙晖; 齐晓龙; 王相国; 邢凯
（北京农学院动物科学技术学院，北京102206）
Author(s):
YUAN Jia-ni; ZHAO Yan-hui; SHI Yu-mei; NI He-min; GUO Yong; SHENG Xi-hui; QI Xiao-long; WANG Xiang-guo; XING Kai
(Animal Science and Technology College, Beijing University of Agriculture, Beijing 102206, China)
关键词:
种公鸡; 精子活力; 加权基因共表达网络（WGCNA）; 核心基因
Keywords:
breeder cocks;  sperm motility; weighted gene co-expression network analysis (WGCNA); hub gene
分类号:
S831.2
DOI:
doi:10.3969/j.issn.1000-4440.2023.03.017
文献标志码:
A
摘要:
种公鸡的精子活力对养禽业的可持续发展至关重要，通过加权基因共表达网络（WGCNA）分析法挖掘种公鸡睾丸、附睾中调控精子活力的基因共表达模块和核心基因，并构建与种公鸡精子活力相关的调控网络。基于团队前期对不同精子活力种公鸡睾丸、附睾组织转录组测序数据的分析，用WGCNA方法构建基因共表达网络，识别与表型性状显著相关的基因模块，并对关键模块基因进行GO功能注释、KEGG通路富集分析。用Cytoscape软件筛选每个关键模块的核心基因并构建可视化共表达网络。结果表明，14 227个基因聚类到11个模块，以决定系数（R2）≥0.6、P＜0.05为标准挖掘出青绿色（Turquoise）模块、黄色（Yellow）模块、红色（Red）模块与表型显著相关。对3个关键模块的基因进行功能分析，发现这些基因显著富集在核苷酸切除修复、同源重组、细胞色素P450对异类物质代谢、MAPK信号通路和细胞凋亡等通路上。选出的IFT家族基因与HMOX2、CYP4B1、ANG、ITGB2基因是与种公鸡精子活力相关的核心基因，可作为提高精子活力的潜在基因。
Abstract:
The sperm motility of breeding roosters is crucial for the sustainable development of the poultry farming. The coexpression modules and core genes regulating sperm motility in testis and epididymis were explored by weighted gene co-expression network analysis (WGCNA), and the regulatory network related to sperm motility in breeder cocks was constructed. The transcriptome sequencing data of testicular and epididymis tissues of breeder cocks with high and low sperm motility were analyzed. The gene co-expression network was constructed by WGCNA method, and gene modules significantly associated with phenotypic traits were identified. GO functional annotation and KEGG pathway enrichment analysis were performed for the module genes. Cytoscape software was used to screen key genes and visualize the co-expression network. The results showed that 14 227 genes were clustered into 11 modules, Turquoise, Yellow and Red modules were mined with R2≥0.6 and P<0.05 as criteria. Functional analysis of the genes in the three key modules showed that these genes were mainly enriched in nucleotide excision repair, homologous recombination, effects of cytochrome P450 on xenobiotic metabolism, MAPK signaling pathway, apoptosis and other signaling pathways. In this study, the selected IFT family genes HMOX2, CYP4B1, ANG and ITGB2 were core genes related to sperm motility of breeder cocks, which could be used as potential genes for improving sperm motility.

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备注/Memo

备注/Memo:
收稿日期：2022-06-25 作者简介：原佳妮（1999-），女，山西晋城人，硕士研究生，研究方向为功能基因组学与生物信息学。(E-mail)BUAYjn@163.com 通讯作者：邢凯，(E-mail)xk181986@163.com
更新日期/Last Update: 2023-07-11