[1]高鹏,王广达,魏兆根,等.水稻热激转录因子家族基因对纹枯病病菌和植物激素的响应特征[J].江苏农业学报,2023,(03):609-621.[doi:doi:10.3969/j.issn.1000-4440.2023.03.001]
　GAO Peng,WANG Guang-da,WEI Zhao-gen,et al.Response characteristics of rice heat shock factors family genes to Rhizoctonia solani and plant hormones[J].,2023,(03):609-621.[doi:doi:10.3969/j.issn.1000-4440.2023.03.001]
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水稻热激转录因子家族基因对纹枯病病菌和植物激素的响应特征()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2023年03期
页码:
609-621
栏目:
遗传育种·生理生化
出版日期:
2023-06-30

文章信息/Info

Title:
Response characteristics of rice heat shock factors family genes to Rhizoctonia solani and plant hormones
作者:
高鹏1; 王广达2; 魏兆根1; 叶元妹1; 冯志明1; 2; 赵剑华1; 居冉1; 谢文亚1; 2; 陈宗祥1; 2; 胡珂鸣1; 2; 左示敏1; 2
（1.扬州大学农学院/江苏省作物基因组学和分子育种重点实验室/植物功能基因组学教育部重点实验室,江苏扬州225009;2.扬州大学/江苏省粮食作物现代产业技术协同创新中心/江苏省作物遗传生理重点实验室,江苏扬州225009）
Author(s):
GAO Peng1; WANG Guang-da2; WEI Zhao-gen1; YE Yuan-mei1; FENG Zhi-ming1; 2; ZHAO Jian-hua1; JU Ran1; XIE Wen-ya1; 2; CHEN Zong-xiang1; 2; HU Ke-ming1; 2; ZUO Shi-min1; 2
(1.Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Key Laboratory of Plant Functional Genomics of Ministry of Education/College of Agriculture, Yangzhou University, Yangzhou 225009, China;2.Jiangsu Collaborative Innovation Center for Modern Industrial Technology of Grain Crops/Jiangsu Key Laboratory of Crop Genetics and Physiology/Yangzhou University, Yangzhou 225009, China)
关键词:
水稻; OsHsf基因; 纹枯病; 防御相关激素
Keywords:
rice; OsHsf gene; sheath blight; defense-related hormone
分类号:
S435.111.4+2
DOI:
doi:10.3969/j.issn.1000-4440.2023.03.001
文献标志码:
A
摘要:
解析水稻热激转录因子(Heat shock factors, Hsf)家族基因响应纹枯病病菌侵染和4种植物激素处理的表达特征，可为进一步解析Hsf调控水稻纹枯病抗性与对相关逆境的响应提供重要依据。利用生物信息学方法搜索并鉴定到25个水稻Hsf基因，对其系统进化树、相关分子特征、蛋白质结构域、基因结构及顺式作用元件进行预测和分析，用荧光定量PCR法分析它们对纹枯病病菌侵染的响应特征及4种激素[茉莉酸(Jasmonic acid，JA)、水杨酸(Salicylic acid，SA)、乙烯(Ethylene，ETH)和激动素(Kinetin，KT）]处理后的表达模式，同时分析它们在水稻组织中的表达情况。结果显示，水稻Hsf蛋白总体上可划分为5组，3个OsHsf亚家族基因在进化关系上的距离较远。25个Hsf蛋白的相对分子质量大小不一，且多数蛋白质的稳定性不高，但其基因结构较为保守。这些基因可能与激素响应及光信号通路相关。有5个基因呈组成型表达，另有7个基因呈组织特异性表达。水稻Hsf基因总体上受纹枯病病菌诱导的强度较低，其中11个上调表达的基因主要集中在OsHsfA、OsHsfB亚家族，4个下调表达的基因主要集中在OsHsfC亚家族, 提示Hsf基因可能在调控水稻对纹枯病抗性的功能上存在分化。4个基因（OsHsfA2a、OsHsfA3、OsHsfB2a、OsHsfB2c）强烈响应纹枯病病菌的侵染，并且在叶鞘、叶片组织中的相对表达量较高，表明这些基因可能参与调控水稻对纹枯病的抗性。多数Hsf基因能够响应4种植物激素处理，总体来说，大部分OsHsf基因在JA、SA和ETH处理下呈下调表达，仅有少数基因呈上调表达；JA处理与SA处理相比，有1个Hsf基因受诱导表达的特征相反，SA处理和ETH处理相比，有3个Hsf基因受诱导表达的特征相反，JA处理和ETH处理相比，有3个Hsf基因受诱导表达的特征相反，JA处理与SA处理相比，SA处理与ETH处理相比，JA处理与ETH处理相比，表达特征相似的基因分别有18个、16个和13个。OsHsfA2a、OsHsfA3、OsHsfB2a、OsHsfB2c等4个水稻的Hsf基因可能参与调控水稻对纹枯病的抗性，研究结果明确了水稻中Hsf基因对不同激素处理的响应特征，为进一步研究Hsf在水稻逆境响应中的功能提供了参考依据。
Abstract:
Characterization of the expression of rice heat shock factors (Hsf) family genes in response to the infection of Rhizoctonia solani and the treatment of four plant hormones can provide an important basis for further analysis of the regulation of sheath blight resistance and related stress response by Hsf. Twenty-five rice Hsf genes were identified by searching using bioinformatics methods, and their phylogenetic tree, molecular characteristics, protein domains, gene structure and cis-acting elements were predicted and analyzed, while the response characteristics to the infection of R. solani and the expression patterns of four plant hormones (jasmonic acid: JA, salicylic acid: SA, ethylene: ETH, kinetin: KT) after treatment, and the expression patterns in different tissues in rice were analyzed through qPCR. Overall, the rice Hsf proteins could be classified into five groups. The three OsHsf subfamily genes were evolutionarily distant from each other. The 25 Hsf proteins had different molecular weights and most of them were unstable, but their gene structures were relatively conserved. These genes might be associated with hormone response as well as light signaling pathways. Five genes were constitutively expressed and seven genes were tissue-specific. In general, rice Hsf genes were less induced by R. solani, with 11 up-regulated genes mainly in the OsHsfA and OsHsfB subfamiliy and four down-regulated genes mainly in the OsHsfC subfamily, suggesting a possible divergence in the evolution of Hsf genes in regulating resistance to sheath blight. Four genes (OsHsfA2a, OsHsfA3, OsHsfB2a and OsHsfB2c) were highly expressed mainly in leaves and leaf sheaths while strongly responding to sheath blight, suggesting that these genes might be involved in regulating resistance to sheath blight. Most Hsf genes were able to respond to the treatments of four plant hormones, and overall, most OsHsf genes showed down-regulated expression in JA, SA and ETH treatments, and only a few showed up-regulated expression. There were one, three and three Hsf with opposite induced expression characteristics in JA and SA, SA and ETH, and JA and ETH treatments, respectively, and 18, 16 and 13 were similarly expressed, respectively. Four rice Hsf genes, including OsHsfA2a, OsHsfA3, OsHsfB2a, OsHsfB2c, might be involved in regulating the resistance to rice sheath blight and the response characteristics of Hsf genes in rice to different plant hormones were clarified, which provided a reference for further studies on the function of Hsf in rice stress response.

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备注/Memo

备注/Memo:
收稿日期：2023-02-01 基金项目：国家自然科学基金项目（31872858、32000362）；江苏省政府种业振兴项目[JBGS(2021)001]；江苏省研究生科研创新计划资助项目（KYCX20_2985）；扬州大学“青蓝工程”项目作者简介：高鹏（1994-），男，江苏常州人，博士研究生，研究方向为水稻抗纹枯病分子育种。（E-mail）yzugaopeng@163.com。王广达为共同第一作者。通讯作者：左示敏，(E-mail)smzuo@yzu.edu.cn;胡珂鸣,(E-mail)hukm@yzu.edu.cn
更新日期/Last Update: 2023-07-11