[1]李颖颖,蒋彦婕,李文奇,等.稻米淀粉体外消化试验方法的优化及应用[J].江苏农业学报,2023,(02):539-546.[doi:doi:10.3969/j.issn.1000-4440.2023.02.028]
 LI Ying-ying,JIANG Yan-jie,LI Wen-qi,et al.Optimization and application of in vitro digestion test method of rice starch[J].,2023,(02):539-546.[doi:doi:10.3969/j.issn.1000-4440.2023.02.028]
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稻米淀粉体外消化试验方法的优化及应用()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2023年02期
页码:
539-546
栏目:
加工贮藏·质量安全
出版日期:
2023-04-30

文章信息/Info

Title:
Optimization and application of in vitro digestion test method of rice starch
作者:
李颖颖12蒋彦婕1李文奇1陶亚军1王芳权1陈智慧1许扬13王军13范方军1
(1.江苏省农业科学院粮食作物研究所,农业农村部淮河下游种质创新重点实验室,江苏南京210014;2.南京农业大学生命科学学院,江苏南京210095;3.江苏省粮食作物现代产业技术协同创新中心,江苏扬州225009)
Author(s):
LI Ying-ying12JIANG Yan-jie1LI Wen-qi1TAO Ya-jun1WANG Fang-quan1CHEN Zhi-hui1XU Yang13WANG Jun13FAN Fang-jun1
(1.Institute of Food Crops, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Germplasm Innovation in Downstream of Huaihe River(Nanjing), Ministry of Agriculture and Rural Affairs of the People’s Republic of China, Nanjing 210014,China;2.College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China;3.Collaborative Innovation Center for Modern Production Technology of Grain Crops of Jiangsu Province, Yangzhou 225009, China)
关键词:
水稻淀粉水解率升糖指数(GI)淀粉体外消化
Keywords:
ricestarch hydrolysis rateglycemic index (GI)starch digestion in vitro
分类号:
S511;R151.2
DOI:
doi:10.3969/j.issn.1000-4440.2023.02.028
文献标志码:
A
摘要:
为实现快捷、经济的稻米升糖指数(GI)估测,首先以不同直链淀粉含量的水稻品种紫金糯2号、南粳04062、宁西优Y16稻米为研究材料,分析了样品前处理方式、α-淀粉酶和淀粉糖苷酶的添加量及方式等对稻米淀粉水解的影响,明确了稻米淀粉体外消化的适宜前处理方式、α-淀粉酶和淀粉糖苷酶的适宜添加量和方式,进而根据淀粉水解指数和水解率进行了稻米升糖指数的估算。结果表明,稻米升糖指数估算适宜的淀粉体外消化试验方法是0.75 g精米加水煮熟后,人工研棒挤压30下,加pH6.8的磷酸盐缓冲液定容至15 ml,同时加入300 U/ml的α-淀粉酶和50 U/ml的淀粉糖苷酶各1 ml联合水解60 min,通过监测水解液葡萄糖含量动态变化,计算1 h内的淀粉水解指数及1 h的水解率,即可实现稻米升糖指数的估算;9个稻米样本的GI估算结果表明本研究建立的试验方法能实现低GI稻米资源和遗传材料的初步筛选。
Abstract:
In order to estimate the glycemic index (GI) of rice quickly and economically, the rice varieties Zijinnuo 2, Nanjing 04062 and Ningxiyou Y16 with different amylose contents were used as the research materials. The effects of sample pretreatment methods, the addition amount and method of α-amylase and amyloglucosidase on the hydrolysis characteristics of rice starch were analyzed. The suitable pretreatment method of rice starch in vitro digestion, the suitable addition amount and method of α-amylase and amyloglucosidase were determined. The GI value of rice was estimated according to the starch hydrolysis index and hydrolysis rate. The results showed that the suitable starch in vitro digestion test scheme for estimating the glycemic index of rice was as follows: 0.75 g of polished rice was boiled with water, then extruded 30 times by artificial stick, and the volume was adjusted to 15 ml with pH 6.8 phosphate buffer. Then, 1 ml 300 U/ml α-amylase and 1 ml 50 U/ml amyloglucosidase were added synchronously to hydrolyze for 60 min. By monitoring the dynamic change of glucose content in the hydrolysate, the GI value of rice could be estimated by the starch hydrolysis index within 1 h and the hydrolysis rate at 1 h. The GI estimation values of nine rice samples indicated that the experimental method established in this study could realize the preliminary screening of low GI rice resources and genetic materials.

参考文献/References:

[1]INTERNATIONAL DIABETES FEDERATION. IDF diabetes atlas(EB/OL).
[2022/04/20]. https://www.diabetesatlas.org/en/.
[2]MICHELLE R T, ASGAR F,ABISHEK B S, et al. Intrinsic and extrinsic factors affecting rice starch digestibility[J]. Trends in Food Science & Technology,2019,88:10-22.
[3]冯博. 低GI与低碳水饮食对超重、肥胖症者的减脂效果[J]. 食品安全导刊,2022(21):134-136.
[4]FOOD AND AGRICULTURE ORGANIZATION OF THE UNITED NATION. Rice market monitor (EB/OL).
[2022/04/20]. https://www.fao.org/markets-and-trade/commodities/rice/fao-rice-price-update/en/
[5]BUTARDO V M,SREENIVASULU N,JULIANO B O. Improving rice grain quality: state-of-the-art and future prospects[J]. Methods in Molecular Biology,2019,1892:19-55.
[6]LEE K Y,LEE H G. Comparative effects of slowly digestible and resistant starch from rice in high-fat diet-induced obese mice[J]. Food Science and Biotechnology,2016,25(5):1443-1448.
[7]TRINIDAD T P,MALLILLIN A C,ENCABO R R,et al. The effect of apparent amylose content and dietary fibre on the glycemic response of different varieties of cooked milled and brown rice[J]. International Journal of Food Sciences and Nutrition,2013,64(1):89-93.
[8]PHYLLIS A C. Fact: The glycemic response to foods[J]. Nutrition Today,1984,19(2):6-11.
[9]JAMES W W,JOHN A M,CHARLES S,et al. Simulating human carbohydrate digestion in vitro: a review of methods and the need for standardisation[J]. International Journal of Food Science & Technology,2008,43(12):2245-2256.
[8] 杨小雪,王丽丽,丁岚,等. 加工方式对红小豆粉理化性质及预估血糖生成指数的影响[J]. 中国粮油学报,2021,36(1):33-38.
[9]GRANFELDT Y,BJRCK I,DREWS A,et al. An in vitro procedure based on chewing to predict metabolic response to starch in cereal and legume products[J]. American Journal of Clinical Nutrition,1994, 59(3):777S.
[10]BUTARDO V M,SREENIVASULU N. Tailoring grain storage reserves for a healthier rice diet and its comparative status with other cereals[J]. International Review of Cell and Molecular Biology,2016,23:31-70.
[11]姜彩霞,汪睿,郑喜群,等. 白芸豆中α-淀粉酶抑制剂的制备及性质研究[J]. 食品科技,2022,47(2):251-255.
[12]李恒,刘静,孙桂菊,等. 抗性淀粉、脂肪和蛋白质对淀粉体外消化速度的影响[J]. 卫生研究,2007(3):308-310.
[13] BUTARDO V M,SREENIVASULU N,JULIANO B O. Improving rice grain quality:State-of-the-art and future prospects[J]. Methods in Molecular Biology,2019,1892:19-55.
[14]张翼麟,谢勇,易川虎,等. 青稞结构对淀粉体外消化的影响[J]. 食品与发酵工业,2021,47(15):98-103.
[15]曾悦. 稻谷类及豆类碳水化合物消化速度与血糖反应的初步研究[D]. 北京:中国农业大学,2005.
[16]KAI L,WANG S,LE C,et al. Discovery of a low-glycemic index potato and relationship with starch digestion in vitro[J]. British Journal of Nutrition,2013,111(4):699-705.
[17]TOUTOUNJI M R, BUTARDO V M ,ZOU W J, et al. A high-throughput in vitro assay for screening rice starch digestibility[J]. Foods, 2019, 8(12):601.
[18]FERNANDES J M,MADALENA D A,PINHEIRO A C, et al. Rice in vitro digestion: application of INFOGEST harmonized protocol for glycemic index determination and starch morphological study[J]. Journal of Food Science and Technology,2020, 57:1393-1404.
[19]JENKINS D J,WOLEVER T M,TAYLOR R H,et al. Glycemic index of foods: a physiological basis for carbohydrate exchange[J]. The American Journal of Clinical Nutrition,1981,34(3):362-366.
[20]FITZGERALD M A,RAHMAN S,RESURRECCION A,et al. Identification of a major genetic determinant of glycemic index in rice[J]. Rice,2011,4(2):66-74.
[21]LIN L S, CAI C H,GILBERT R G,et al. Relationships between amylopectin molecular structures and functional properties of different-sized fractions of normal and high-amylose maize starches[J]. Food Hydrocolloids,2016,52:359-368.
[22]GOI I,GARCIA-ALONSO A,SAURA-CALIXTO F. A starch hydrolysis procedure to estimate glycemic index[J]. Nutrition Research,1997,17(3):427-437.
[23]中华人民共和国国家质量监督检验检疫总局,中国国家标准化管理委员会. 优质稻谷GB/T17891-2017[S]. 北京:中国标准出版社,2017.
[24]刘辉,王成祥,刘兵军,等. 一种血糖生成指数体外检测方法:CN105911007A[P]. 2016-08-31.
[25]顾娟,洪雁,顾正彪. 荞麦淀粉理化性质的研究[J]. 食品与发酵工业,2008(4):36-39.
[26] AHMED R, SEGAL I, HASSAN H. Fermentation of dietary starch in humans [J]. American Journal of Gastroenterology, 2000,95 (4): 1017-1020
[27]徐箐,柳嘉,林静,等. 低GI淀粉原料的筛选及理化特性和体外消化特性的研究[J]. 食品研究与开发,2020,41(20):8-14.
[28]LI C,YU W W,WU P,et al. Current in vitro digestion systems for understanding food digestion in human upper gastrointestinal tract[J]. Trends in Food Science & Technology,2020,96:114-126.
[29]PAN Z,YE A Q,LI S,et al. Dynamic in vitro gastric digestion of sheep milk: Influence of homogenization and heat treatment[J]. Foods,2021,10(8):1938.
[30]CAI Y D,QIN W,SUNANTHA K,et al. Impact of particle size of pulverized citrus peel tissue on changes in antioxidant properties of digested fluids during simulated in vitro digestion[J]. Food Science and Human Wellness,2020,9(1):58-63.
[31]TSUGAMI Y,SUZUKI N,KAWAHARA M,et al. Establishment of an in vitro culture model to study milk production and the blood-milk barrier with bovine mammary epithelial cells[J]. Animal Science Journal,2020,91(1):e13355.
[32]MUIR J G,O’DEA K. Measurement of resistant starch: factors affecting the amount of starch escaping digestion in vitro[J]. American Journal of Clinical Nutrition,1992,56(1):123-127.
[33]曾悦,刘芳,刘波,等. 一种淀粉类食品消化速度体外测定法的探讨及应用[J]. 食品与发酵工业,2005(5):114-118.
[34]王淑颖,范志红,刘波. 不同品种稻米中碳水化合物的消化特性[J]. 中国农业大学学报,2012,17(4):129-134.
[35]RITUDOMPHOL O,LUANGSAKUL N. Optimization of processing condition of instant rice to lower the Glycemic Index[J]. Journal of Food Science,2019,84(1):101-110.
[36]RANAWANA D,HENRY C J,LIGHTOWLER H J,et al. Glycaemic index of some commercially available rice and rice products in Great Britain[J]. International Journal of Food Sciences and Nutrition,2009,60(4):99-110.
[37]ARAYA H,CONTRERAS P,ALVIA M,et al. A comparison between an in vitro method to determine carbohydrate digestion rate and the glycemic response in young men[J]. European Journal of Clinical Nutrition,2002,56(8):735-739.
[38]JULIANO B O. Rice: Chemistry and technology[M]. St Paul, MN: American Association of Cereal Chemists,1985
[39] BUTARDO V M, SREENIVASULU N, JULIANO B O. Rice grain quality: State of the art and future prospects[M]//SREENIVASULU N. Rice grain quality: Methods and protocols. Clifton: Humana Press,2019
[40] STROCCHI A, LEVITT M D. Measurement of starch absorption in humans[J]. Canadian Journal of Physiology and Pharmacology, 1991,69:108-110.

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备注/Memo

备注/Memo:
收稿日期:2022-04-20 基金项目:江苏省农业科技自主创新基金项目[CX(20)3008];江苏省重点研发计划(现代农业)项目(BE2020);国家自然科学基金项目(31571585) 作者简介:李颖颖(1998-),女,安徽宿州人,硕士研究生,研究方向为水稻营养。(E-mail)2361350374@qq.com 通讯作者:李霞,(E-mail)jspplx@ jaas.ac.cn;杨杰,(E-mail)yangjie168@aliyun.com
更新日期/Last Update: 2023-05-15