[1]江群,凌溪铁,唐兆成,等.EMS诱变创制水稻抗乙酰辅酶A羧化酶抑制剂类除草剂种质[J].江苏农业学报,2023,(02):305-312.[doi:doi:10.3969/j.issn.1000-4440.2023.02.001]
　JIANG Qun,LING Xi-tie,TANG Zhao-cheng,et al.EMS mutagenesis to create rice anti-acetyl-CoA carboxylase inhibitor-herbicide germplasm[J].,2023,(02):305-312.[doi:doi:10.3969/j.issn.1000-4440.2023.02.001]
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EMS诱变创制水稻抗乙酰辅酶A羧化酶抑制剂类除草剂种质()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2023年02期
页码:
305-312
栏目:
遗传育种·生理生化
出版日期:
2023-04-30

文章信息/Info

Title:
EMS mutagenesis to create rice anti-acetyl-CoA carboxylase inhibitor-herbicide germplasm
作者:
江群1; 凌溪铁2; 唐兆成2; 周珍珍2; 张保龙1; 2
（1.海南大学热带作物学院/三亚南繁研究院，海南海口570228；2.江苏省农业科学院种质资源与生物技术研究所/江苏省农业生物学重点实验室，江苏南京210014）
Author(s):
JIANG Qun1; LING Xi-tie2; TANG Zhao-cheng2; ZHOU Zhen-zhen2; ZHANG Bao-long1; 2
(1.College of Tropical Crops/Sanya Nanfan Research Institute, Hainan University, Haikou 570228, China;2.Institute of Germplasm Resources and Biotechnology/Provincial Key Laboratory of Agrobiology, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China)
关键词:
水稻; 抗除草剂种质; 甲基磺酸乙酯（EMS）; 乙酰辅酶A羧化酶
Keywords:
rice; herbicide-resistant germplasm; ethyl methyl sulfonate (EMS); acetyl CoA carboxylase
分类号:
S335.3
DOI:
doi:10.3969/j.issn.1000-4440.2023.02.001
文献标志码:
A
摘要:
创制非转基因抗除草剂水稻种质资源对于稻田杂草防控具有重要价值。本研究以甲基磺酸乙酯（EMS）水溶液诱变镇糯19水稻种子，获得1株能稳定遗传的可耐受乙酰辅酶A羧化酶（ACCase）抑制剂类除草剂高效盖草能的M3代水稻幼苗（突变体）。分别扩增镇糯19野生型和突变体的基因组DNA并进行测序和序列比对，发现突变体ACCase基因的开放阅读框（ORF）的第5 374位碱基发生了点突变，导致编码的第1 792位氨基酸由异亮氨酸突变为亮氨酸。镇糯19野生型和突变体分蘖盛期大田喷施3种田间推荐剂量的ACCase抑制剂类除草剂后农艺性状调查结果表明突变体对高效盖草能、精喹禾灵和唑啉草酯抗性明显高于野生型。本研究获得了能稳定遗传的非转基因抗ACCase抑制剂类水稻新种质，具有一定的应用价值，为抗除草剂水稻育种提供了种质资源。
Abstract:
Cultivating non-transgenic herbicide-resistant rice germplasm resources is of great value for weed control in rice fields. In this study, Zhennuo 19 rice seeds were mutagenized by ethyl methyl sulfonate (EMS) solution, and a M3 generation of rice seedlings with stable inheritance and tolerance to acetyl-CoA carboxylase (ACCase) inhibitor herbicides were obtained. The genomic DNAs of wild-type and the mutant were amplified and sequenced respectively. It was found that there was a point mutation at the 5 374th base of the open reading frame of the resistant rice ACCase gene, resulting in a mutation of the encoded 1 792th amino acid from isoleucine to leucine. Three kinds of ACCase inhibitor herbicides were sprayed in the field and the agronomic traits were analyzed. The results showed that the resistance of the mutant to haloxyfop-R-methyl, quizalofop-P-ethyl and pinoxaden was significantly higher than that of wild type. In this study, a new non-transgenic rice germplasm with ACCase inhibitor resistance was obtained, which had certain application value and could provide germplasm resources for herbicide-resistant rice breeding.

参考文献/References:

[1]董立尧，高原，房加鹏，等. 我国水稻田杂草抗药性研究进展[J]. 植物保护，2018，44(5)：69-76.
[2]程艳勤. 浅析除草剂对水稻的危害及治理[J]. 农技服务，2016，33(6)：109-114.
[3]KONISHI T K U J, SHINOHARA K, YAMADA K, et al. Acetyl-CoA carboxylase in higher plants: most plants other than gramineae have both the prokaryotic and the eukaryotic forms of this enzyme[J]. Plant and Cell Physiology，1996，37(2)：117-122.
[4]王爽，张荣全，叶非. 乙酰辅酶A羧化酶抑制剂的研究进展[J]. 农药科学与管理，2003(10)：26-32.
[5]蔡靖萱. 扬州市小麦田菵草对ACCase抑制剂的抗性研究[D]. 扬州：扬州大学，2020.
[6]RENDINA A R, FELTS J M. Cyclohexanedione Herbicides are selective and potent inhibitors of acetyl-CoA carboxylase from grasses[J]. Plant Physiol，1988，86(4)：983-986.
[7]袁国徽，王恒智，赵宁，等. 耿氏硬草对乙酰辅酶A羧化酶类除草剂抗性水平及分子机制初探[J]. 农药学学报，2016，18(3)：304-310.
[8]董元海. 新苯基吡唑啉类除草剂唑啉草酯的合成[D]. 武汉：武汉工程大学, 2017.
[9]刘博宏，叶非. 芳氧苯氧基丙酸酯类除草剂的应用进展[J]. 农药科学与管理，2011，32(2)：20-25.
[10]吕军，刘军，姜秀英，等. EMS诱导水稻‘辽星1号′突变体的筛选与鉴定[J]. 分子植物育种，2022，20(12)： 4038-4043.
[11]董颖苹，连勇，何庆才，等. 植物化学诱变技术在育种中的运用及进展Ⅱ.突变体的筛选及分子检测[J]. 种子，2005，24(8)：54-58.
[12]黄静. 水稻EMS诱变效率和品种内遗传多态性分析[D]. 福州：福建农林大学, 2015.
[13]顾佳清，张智奇，周音，等. EMS诱导水稻中花11突变体的筛选和鉴定[J]. 上海农业学报，2005，21(1)：7-11.
[14]陈忠明，王秀娥. 水稻强优势恢复系9311粒重的诱变改良[J]. 分子植物育种，2005，3(3)：353-356.
[15]陈忠明，王秀娥，胡兴雨，等. 水稻长穗颈恢复系9311eR的诱变选育[J]. 江苏农业科学，2005(4)：9-11.
[16]陈天子，余月，凌溪铁，等. EMS诱变水稻创制抗咪唑啉酮除草剂种质[J]. 核农学报，2021，35(2)：253-261.
[17]DLYE C, CALMS , MATJICEK A. SNP markers for black-grass (Alopecurus myosuroides Huds.) genotypes resistant to acetyl CoA-carboxylase inhibiting herbicides[J]. Theoretical and Applied Genetics，2002， 104(6)：1114-1120.
[18]DLYE C, ZHANG X, CHALOPIN C, et al. An isoleucine residue within the carboxyl-transferase domain of multidomain acetyl-coenzyme A carboxylase is a major determinant of sensitivity to aryloxyphenoxypropionate but not to cyclohexanedione inhibitors[J]. Plant Physiology，2003，132(3)：1716-1723.
[19]DLYE C, ZHANG X, MICHEL S, et al. Molecular bases for sensitivity to acetyl-coenzyme A carboxylase inhibitors in black-grass[J]. Plant Physiology，2005，137(3)：794-806.
[20]POWLES S B, YU Q. Evolution in Action: plants resistant to herbicides[J]. Annual Review of Plant Biology，2010，61(1)：317-347.
[21]袁国徽，田志慧，高原，等. 上海市水稻田千金子对3种乙酰辅酶A羧化酶抑制剂的抗性现状及酶突变机制[J]. 农药学学报，2022，24(3)：492-500.
[22]BECKIE H J, TARDIF F J. Herbicide cross resistance in weeds[J]. Crop Protection，2012, 35: 15-28.
[23]DENG W, CAI J, ZHANG J, et al. Molecular basis of resistance to ACCase-inhibiting herbicide cyhalofop-butyl in Chinese sprangletop (Leptochloa chinensis (L.) Nees) from China[J]. Pestic Biochem Physiol，2019，158：143-148.
[24]PENG Y, PAN L, LIU D, et al. Confirmation and characterization of cyhalofop-butyl-resistant Chinese sprangletop (Leptochloa chinensis ) populations from China[J]. Weed Science，2020, 68(3): 253-259.
[25]张怡，陈丽萍，徐笔奇，等. 浙江稻区千金子对氰氟草酯和噁唑酰草胺的抗药性及其分子机制研究[J]. 农药学学报，2020，22(3)：447-453.
[26]VILA AIUB M M, NEVE P, POWLES S B. Resistance cost of a cytochrome P450 herbicide metabolism mechanism but not an ACCase target site mutation in a multiple resistant Lolium rigidum population[J]. New Phytologist，2005，167(3)：787-796.
[27]MENCHARI Y, CHAUVEL B, DARMENCY H, et al. Fitness costs associated with three mutant acetyl-coenzyme A carboxylase alleles endowing herbicide resistance in black-grass Alopecurus myosuroides[J]. Journal of Applied Ecology，2008，45(3)：939-947.
[28]WANG T, PICARD J C, TIAN X, et al. A herbicide-resistant ACCase 1781 setaria mutant shows higher fitness than wild type[J]. Heredity (Edinb)，2010，105(4)：394-400.
[29]SERRAT X, ESTEBAN R, GUIBOURT N, et al. EMS mutagenesis in mature seed-derived rice calli as a new method for rapidly obtaining TILLING mutant populations[J]. Plant Methods，2014，10(1)：5.
[30]SHA X Y, LINSCOMBE S D, GROTH D E. Field evaluation of imidazolinone-tolerant Clearfield rice (Oryza sativa L.) at nine Louisiana locations[J]. Crop Science，2007，47(3)：1177-1185.
[31]CAMACHO J R, LINSCOMBE S D, SANABRIA Y, et al. Inheritance of Provisia rice resistance to quizalofop-p-ethyl under laboratory and greenhouse environments[J]. Euphytica，2019，215(4)：83.

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备注/Memo

备注/Memo:
收稿日期：2022-11-25 基金项目：江苏省农业科技自主创新基金项目[CX(21)2041] 作者简介：江群（1998-），女，四川宜宾人，硕士研究生，主要从事水稻抗除草剂育种研究。（E-mail）1692579264@qq.com 通讯作者：张保龙，（E-mail）zhbl2248@hotmail.com；周珍珍，（E-mail）zhenzhenzhounj@163.com
更新日期/Last Update: 2023-05-12