[1]王会文,范军强,路晓明,等.白菜型冬油菜NAC基因家族鉴定及表达分析[J].江苏农业学报,2022,38(05):1315-1329.[doi:doi:10.3969/j.issn.1000-4440.2022.05.019]
　WANG Hui-wen,FAN Jun-qiang,LU Xiao-ming,et al.Identification and expression analysis of NAC gene family in Brassica rapa L.[J].,2022,38(05):1315-1329.[doi:doi:10.3969/j.issn.1000-4440.2022.05.019]
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白菜型冬油菜NAC基因家族鉴定及表达分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
38
期数:
2022年05期
页码:
1315-1329
栏目:
园艺
出版日期:
2022-10-31

文章信息/Info

Title:
Identification and expression analysis of NAC gene family in Brassica rapa L.
作者:
王会文1; 2; 范军强1; 2; 路晓明1; 2; 曾瑞1; 2; 武军艳1; 2; 刘丽君2; 马骊2; 蒲媛媛1; 2; 孙万仓1; 2; 李学才1; 2
(1.甘肃农业大学农学院，甘肃兰州730070；2.省部共建干旱生境作物学国家重点实验室，甘肃兰州730070)
Author(s):
WANG Hui-wen1; 2; FAN Jun-qiang1; 2; LU Xiao-ming1; 2; ZENG Rui1; 2; WU Jun-yan1; 2; LIU LI-jun2; MA Li2; PU Yuan-yuan1; 2; SUN Wan-cang1; 2; LI Xue-cai1; 2
(1.College of Agronomy, Gansu Agricultural University, Lanzhou 730070， China;2.State Key Laboratory of Aridland Crop Science, Lanzhou 730070, China)
关键词:
白菜型冬油菜; NAC转录因子; 家族分析; 非生物胁迫; 表达分析
Keywords:
Brassica rapa L.; NAC transcription factor; family analysis; abiotic stress; expression analysis
分类号:
S565.4
DOI:
doi:10.3969/j.issn.1000-4440.2022.05.019
文献标志码:
A
摘要:
NAC(NAM、ATAF1/2、CUC2)是植物特有的一类转录因子基因家族，其家族成员在参与响应低温、干旱等非生物胁迫过程发挥重要作用。本研究基于白菜型冬油菜陇油7号全基因组测序结果，共鉴定出119个NAC家族成员，系统进化结果显示，所有NAC转录因子基因聚类为8个亚族，在10条染色体上呈不均匀分布；BraNAC蛋白在细胞核、细胞质、叶绿体、线粒体等细胞器中都有分布；启动子作用元件分析结果显示，BraNAC家族成员广泛参与低温胁迫响应、干旱胁迫响应、机械损伤响应、胚胎发育和内源激素调控等进程；对BraNAC基因在低温胁迫下白菜型冬油菜生长锥中的表达预测结果表明，大部分BraNAC基因通过上调表达使白菜型冬油菜适应低温环境。在低温和干旱胁迫下，选择11个BraNAC基因在2个耐寒性不同的白菜型冬油菜品种的幼苗中进行表达分析，发现BraNAC家族基因受不同逆境胁迫诱导，并且在不同处理时间与植物不同组织中存在表达特异性。本研究扩展了植物NAC基因家族成员信息，为鉴定和筛选抗逆基因提供参考。
Abstract:
NAC (NAM, ATAF1/2, CUC2) is a plant-specific transcription factor gene family, and its family members play an important role in response to abiotic stresses such as low temperature and drought. In this study, a total of 119 NAC family genes were identified based on the whole genome sequencing results of Brassica rapa L. Longyou 7. Phylogenetic analysis showed that all NAC transcription factor genes were clustered into eight subfamilies and were unevenly distributed on ten chromosomes. The BraNAC proteins were located in the nucleus, cytoplasm, chloroplast, mitochondrion and other organelles. The analysis of promoter elements showed that BraNAC family members were widely involved in low temperature stress response, drought stress response, mechanical damage response, embryonic development and endogenous hormone regulation. The expression prediction of BraNAC genes in the growth cones of Brassica rapa L. under low temperature stress showed that most BraNAC genes adapted to low temperature environment by up-regulating expression. Under low temperature and drought stress, 11 BraNAC genes were selected for expression analysis in the seedlings of two Brassica rapa L. varieties with different cold tolerance, and it was found that the BraNAC family genes were induced by different stresses and had tissue and time expression specificity. In this study, the information of plant NAC gene family members is expanded, which provides reference for identification and screening of stress resistance genes.

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备注/Memo

备注/Memo:
收稿日期：2022-03-06基金项目：甘肃农业大学干旱生境作物学重点实验室主任基金项目(GSCS-2020-Z1)；国家现代农业产业技术体系项目(CARS-12)；国家自然科学基金项目(31860388、31960435)作者简介：王会文(1998-)，男，甘肃成县人，硕士研究生，研究方向为作物遗传育种。(E-mail)2569615823@qq.com通讯作者：李学才,(E-mail)348930135@qq.com
更新日期/Last Update: 2022-11-07