[1]刘蓓一,韦青,田吉鹏,等.羊瘤胃液中产阿魏酸酯酶乳酸菌的筛选及对水稻秸秆青贮品质的影响[J].江苏农业学报,2022,38(05):1306-1314.[doi:doi:10.3969/j.issn.1000-4440.2022.05.018]
　LIU Bei-yi,WEI Qing,TIAN Ji-peng,et al.Screening of ferulic acid esterase-producing lactic acid bacteria from rumen fluid of sheep and their effects on silage quality of rice straw[J].,2022,38(05):1306-1314.[doi:doi:10.3969/j.issn.1000-4440.2022.05.018]
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羊瘤胃液中产阿魏酸酯酶乳酸菌的筛选及对水稻秸秆青贮品质的影响()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
38
期数:
2022年05期
页码:
1306-1314
栏目:
畜牧兽医·水产养殖
出版日期:
2022-10-31

文章信息/Info

Title:
Screening of ferulic acid esterase-producing lactic acid bacteria from rumen fluid of sheep and their effects on silage quality of rice straw
作者:
刘蓓一1; 2; 韦青1; 2; 田吉鹏1; 2; 程云辉1; 2; 许能祥1; 2; 张文洁1; 2; 顾洪如1; 2; 路庆鹏3; 刘翔4; 丁成龙1; 2
（1.江苏省农业科学院畜牧研究所，江苏南京210014；2.江苏省农业科学院农业农村部种养结合重点实验室，江苏南京210014；3.江苏绿科生物技术有限公司，江苏扬州225600；4.睢宁县飞翔牧业有限公司，江苏睢宁221243）
Author(s):
LIU Bei-yi1; 2; WEI Qing1; 2; TIAN Ji-peng1; 2; CHENG Yun-hui1; 2; XU Neng-xiang1; 2; ZHANG Wen-jie1; 2; GU Hong-ru1; 2; LU Qing-peng3; LIU Xiang4; DING Cheng-long1; 2<
(1.Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nangjing 210014, China;2.Key Laboratory of Crop and Livestock Integrated Farming, Ministry of Agriculture and Rural Affairs, Nangjing 210014, China;3.Jiangsu Lüke Biotechnology Co., Ltd., Yangzhou 225600, China;4.Suining Feixiang Animal Husbandry Co., Ltd., Suining 221243, China)
关键词:
瘤胃液; 阿魏酸酯酶; 乳酸菌; 水稻秸秆; 青贮饲料
Keywords:
rumen fluid; ferulic acid esterase; lactic acid bacteria; rice straw; silage
分类号:
S826.8+9
DOI:
doi:10.3969/j.issn.1000-4440.2022.05.018
文献标志码:
A
摘要:
为了进一步降解水稻秸秆青贮饲料中的纤维，提高青贮饲料品质，从湖羊瘤胃液中筛选产阿魏酸酯酶的乳酸菌，鉴定菌株特性，并将其接种到水稻秸秆青贮中，研究其对青贮水稻秸秆纤维降解率和化学成分的影响。结果表明，从湖羊瘤胃液中共筛选出2株高产阿魏酸酯酶的乳酸菌，菌株SR1与鼠李糖乳杆菌的同源性最高，SR2与香肠乳杆菌的同源性最高；SR2的产酸速度比SR1快，培养24 h后培养液的pH值为3.83；2株乳酸菌在pH值4.0～7.0、温度25～37 ℃、3.0% NaCl条件下的生长状况良好，都能利用多种糖源物质。与CK相比，接种菌株SR2组的水稻秸秆青贮饲料中性洗涤纤维、酸性洗涤纤维和纤维素含量均显著降低（P<0.05），而接种菌株SR1对水稻秸秆青贮饲料纤维含量的影响不大；接种菌株SR2组的水稻秸秆青贮饲料pH值、铵态氮含量最低，乳酸含量最高。综上，香肠乳杆菌SR2可作为水稻秸秆青贮的备选菌株。
Abstract:
In order to further degrade the fiber in rice straw silage and improve the quality of silage, ferulic acid esterase-producing lactic acid bacteria were isolated from rumen fluid of Hu sheep, and their characteristics were identified. The strains were inoculated into rice straw silage to study their effects on fiber degradation rate and chemical composition of rice straw silage. The results showed that two strains of lactic acid bacteria with high ferulic acid esterase were isolated from rumen fluid of Hu sheep. Strain SR1 had the highest homology with Lactobacillus rhamnosus, and SR2 had the highest homology with Lactobacillus farciminis. The acid production rate of strain SR2 was faster than that of strain SR1, and the final pH value was 3.83. The two strains of lactic acid bacteria grew well under the conditions of pH 4.0-7.0, temperature 25-37 ℃ and 3.0% NaCl, and they could use various sugar sources. Compared with CK, the contents of neutral detergent fiber, acid detergent fiber and cellulose in the inoculated strain SR2 group decreased significantly （P<0.05）, but the inoculated strain SR1 had little effect on the fiber content of rice straw silage. The pH value and ammonium nitrogen content of rice straw silage in the inoculated strain SR2 group were the lowest, and the lactic acid content was the highest. In conclusion, Lactobacillus farciminis SR2 can be used as an alternative strain for rice straw silage.

参考文献/References:

[1]张晓庆,王梓凡,参木友,等.中国农作物秸秆产量及综合利用现状分析[J].中国农业大学学报, 2021, 26(9): 30-41.
[2]李双双,赵辉. 微生物阿魏酸酯酶性质及应用研究进展[J].食品与发酵科技, 2020, 56(5):75-80.
[3]WILSON D B. Microbial diversity of cellulose hydrolysis[J]. Current Opinion in Microbiology, 2011, 14(3): 259-263.
[4]CAO B B, WANG R, YANG H J, et al. In situ ruminal degradation of phenolic acid, cellulose and hemicellulose in crop brans and husks differing in ferulic and p-coumaric acid patters[J]. Journal of Agricultural Science, 2015, 153(7): 1312-1320.
[5]马松成,陈静,毛华明. 瘤胃微生态系统[J]. 中国畜牧兽医, 2007, 34(1):31-34.
[6]李君风,原现军,董志浩,等.西藏地区牦牛瘤胃中兼性厌氧纤维素降解菌的分离鉴定[J].草业学报,2017,26(6):176-184.
[7]DONAGHY J, KELLY P F, MCKAY A M. Detection of ferulic acid esterase production by Bacillus spp. and lactobacilli[J]. Applied Microbiology and Biotechnology, 1998, 50(2):257-260.
[8]杨杨,石超,郭旭生. 高寒草甸魏斯氏乳酸菌的分离鉴定及理化特性研究[J]. 草业学报, 2014, 23(1): 266-275.
[9]凌代文. 乳酸菌分类鉴定及实验方法[M]. 北京: 中国轻工业出版社，1999: 1-25.
[10]LIU B Y, HUAN H L, GU H R, et al. Dynamics of a microbial community during ensiling and upon aerobic exposure in lactic acid bacteria inoculation-treated and untreated barley silages[J]. Bioresource Technology, 2019, 273: 212-219.
[11]BRODERICK G A, KANG J H. Automated simultaneous determination of ammonia and total amino acids in ruminal fluid and in vitro media[J]. Journal of Dairy Science, 1980, 63(1): 64-75.
[12]袁洁,马冉冉,张文洁,等. 自然青贮多花黑麦草优良乳酸菌的筛选及对多花黑麦草青贮品质的影响[J]. 草业学报, 2021, 30(11): 132-143.
[13]VAN SOEST P J, ROBERTSON J B, LEWIS B A. Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition[J]. Journal of Dairy Science, 1991, 74(10):3583-3597.
[14]Association of Official Analytical Chemists. Official methods of analysis[Z]. 15th Ed. Association of Official Analytical Chemists, Artington, Virginia, USA. 1990.
[15]DUBOS M, GILLES K A, HAMILTON J K, et al. Colorimetric method for determination of sugars and related substances [J]. Analytical Chemistry, 1956, 28(3):350-353.
[16]GOTO I, MINSON D J. Prediction of the day matter digestibility of tropical grasses using a pepsin-cellulase assay[J]. Animal Feed Science and Technology, 1977, 2(3): 247-253.
[17]RUSSELL J B, RYCHLIK J L. Factors that alter rumen microbial ecology[J]. Science, 2001, 292(5519): 1119-1122.
[18]买尔哈巴·艾合买提,樊振,李越中,等. 瘤胃中纤维素分解菌的分离、鉴定及其产酶条件的优化[J]. 微生物学报, 2013, 53(5): 470-477.
[19]李干. 产阿魏酸酯酶菌筛选、培养条件及酶学性质研究[D]. 南京：南京林业大学，2011.
[20]卢颖欣,张宁,欧仕益,等. 产阿魏酸酯酶乳酸菌的乳杆菌筛选以及基因克隆[J]. 食品工业科技, 2013, 34(20): 199-203.
[21]荆佩欣.产阿魏酸酯酶乳酸菌的筛选、酶学特性及其在苜蓿青贮中的应用研究[D].兰州：兰州大学,2017.
[22]薛银刚. 鼠李糖乳杆菌的筛选、鉴定及加工特性研究[D]. 扬州：扬州大学, 2006.
[23]章德法,徐为民,徐幸莲. 香肠乳杆菌增殖培养基及培养条件的优化研究[J].食品工业科技, 2009, 30(1): 147-149.
[24]MUCK R E, NADEAU E M G, MCALLISTER T A, et al. Silage review: recent advances and future uses of silage additives[J]. Journal of Dairy Science, 2018, 101(5): 3980-4000.
[25]ZHOU Y, DROUIN P, LAFRENIRE C. Effect of temperature (5-25 ℃) on epiphytic lactic acid bacteria populations and fermentation of whole-plant corn silage[J]. Journal of Applied Microbiology, 2016, 121(3): 657-671.
[26]KUNG L, RANJIT N K. The effect of Lactobacillus buchneri and other additives on the fermentation and aerobic stability of barley silage[J]. Journal of Dairy Science, 2001, 84(5):1149-1155.
[27]FAN X M, ZHAO S S, YANG F Y, et al. Effects of lactic acid bacterial inoculants on fermentation quality, bacterial community, and mycotoxins of alfalfa silage under vacuum or nonvacuum treatment[J]. Microorganisms, 2021, 9(12): 2614.
[28]贾婷婷,吴哲,玉柱. 不同类型乳酸菌添加剂对燕麦青贮品质和有氧稳定性的影响[J].草业科学, 2018, 35(5): 1266-1272.
[29]MUCK R E. Silage microbiology and its control through additives[J]. Revista Brasileira de Zootecnia, 2010, 39(Suppl special): 183-191.
[30]FILYA I, MUCK R E, CONTRERAS-GOVEA F E. Inoculant effects on alfalfa silage: fermentation products and nutritive value[J]. Journal of Dairy Science, 2007, 90(11):5108-5114.
[31]华金玲. 添加乳酸菌对整株水稻秸青贮发酵品质的影响[D]. 哈尔滨：东北农业大学, 2006.
[32]TIAN J P, XU N X, LIU B Y, et al. Interaction effect of silo density and additives on the fermentation quality, microbial counts, chemical composition and in vitro degradability of rice straw silage[J]. Bioresource Technology, 2020, 297:122412.
[33]LYNCH J P, JIN L, LARA E C, et al. The effect of exogenous fibrolytic enzymes and a ferulic acid esterase-producing inoculant on the fibre degradability, chemical composition and conservation characteristics of alfalfa silage[J]. Animal Feed Science and Technology, 2014, 193: 21-31.
[34]NSEREKO V L, SMILEY B K, RUTHERFORD W M, et al. Influence of inoculating forage with lactic acid bacterial strains that produce ferulate esterase on ensilage and ruminal degradation of fiber[J]. Animal Feed Science and Technology, 2008, 145(1):122-135.

备注/Memo

备注/Memo:
收稿日期：2022-05-19基金项目：江苏省农业科技自主创新基金项目[CX(20)3156]；扬州市现代农业项目（YZ2020050）；江苏省苏北科技专项（XZ-SZ202017）；江苏现代农业产业技术体系建设专项资金项目[JATS(2022)440]作者简介：刘蓓一（1984-），女，江苏武进人，博士，副研究员，从事饲草调制与草食动物营养研究。(E-mail)byliu@jaas.ac.cn。韦青为共同第一作者。通讯作者：丁成龙，(E-mail)dingcl@jaas.ac.cn
更新日期/Last Update: 2022-11-07