[1]马李广,张贺龙,庞小可,等.白菜bZIP转录因子基因家族应答春化反应关键基因表达分析[J].江苏农业学报,2022,38(03):765-774.[doi:doi:10.3969/j.issn.1000-4440.2022.03.023]
　MA Li-guang,ZHANG He-long,PANG Xiao-ke,et al.Genome-wide identification of bZIP transcription factor gene family in Brassica rapa and its association with vernalization[J].,2022,38(03):765-774.[doi:doi:10.3969/j.issn.1000-4440.2022.03.023]
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白菜bZIP转录因子基因家族应答春化反应关键基因表达分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
38
期数:
2022年03期
页码:
765-774
栏目:
园艺
出版日期:
2022-06-30

文章信息/Info

Title:
Genome-wide identification of bZIP transcription factor gene family in Brassica rapa and its association with vernalization
作者:
马李广1; 张贺龙1; 庞小可1; 王浩1; 李广1; 2; 汪承刚1; 2; 3; 袁凌云1; 2; 侯金锋1; 2; 唐小燕1; 2; 陈国户1; 2; 3
（1.安徽农业大学园艺学院，安徽省园艺作物育种工程实验室，安徽合肥230036；2.安徽省皖江蔬菜产业研究院，安徽马鞍山238200；3.安徽农业大学皖西南综合试验站，安徽怀宁246100）
Author(s):
MA Li-guang1; ZHANG He-long1; PANG Xiao-ke1; WANG Hao1; LI Guang1; 2; WANG Cheng-gang1; 2; 3; YUAN Ling-yun1; 2; HOU Jin-feng1; 2; TANG Xiao-yan1; 2; CHEN Guo-hu1; 2; 3
（1.Anhui Provincial Engineering Laboratory of Horticultural Crop Breeding, School of Horticulture, Anhui Agricultural University, Hefei 230036, China;2.Wanjiang Vegetable Industrial Technology Institute, Maanshan 238200, China;3.Anhui Southwest Comprehensive Experimental Station of Anhui Agricultural University, Huaining 246100, China）
关键词:
白菜; bZIP转录因子; 基因家族; 春化反应; 生物信息学分析
Keywords:
Brassica rapa; bZIP transcription factor; gene family; vernalization; bioinformatics analysis
分类号:
S634.3
DOI:
doi:10.3969/j.issn.1000-4440.2022.03.023
文献标志码:
A
摘要:
为研究白菜（Brassica rapa）碱性亮氨酸拉链（Basic leucine zipper，bZIP）转录因子家族基因（BrbZIP）的相关功能，通过生物信息学分析技术，鉴定全基因组的BrbZIP基因家族成员，并对其染色体分布、进化关系、表达模式及其应答春化反应等进行了分析。白菜bZIP转录因子基因家族共有118个成员，在染色体上不均等分布。白菜组织转录组分析结果显示，大部分BrbZIP基因在根、茎、叶、花及荚中均有较高的表达丰度，且具有组织表达特异性；春化反应转录组、荧光定量PCR及相关基因互作网络分析结果表明，白菜bZIP基因家族中应答春化反应相关基因上调与下调表达基因数量差异不大，相互之间存在复杂的互作网络，其中4个成员（Bra039631、Bra020620、Bra004550与Bra020471）是应答春化反应的中心节点。
Abstract:
In order to explore the function of basic leucine zipper (bZIP) transcription factor in Brassica rapa and its association with vernalization, bioinformation methods were used to identify the B. rapa bZIP gene family members. The chromosome location, evolutionary relationship, expression pattern and vernalization response were analyzed through a variety of biological information softwares. The results revealed that 118 BrbZIP transcription factors were unevenly distributed on the chromosomes of B. rapa. Transcriptome analysis showed that most of the BrbZIP genes had high expression levels in roots, stems, leaves, flowers and pods, and had tissue expression specificity. The results of transcriptome, fluorescence quantitative PCR and interaction network analysis suggested that there was n o significant difference in the number of up-regulated an d down-regulated genes responding to vernalization in bZIP gene family of B. rapa, and the interaction network was complex. Four BrbZIP genes were the central nodes in response to vernalization.

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备注/Memo

备注/Memo:
收稿日期：2021-09-10基金项目：国家自然科学基金项目（31801853）；国家大学生创新创业基金项目（202110364080）；安徽省大学生创新创业基金项目（S202010364238、S202010364239）；安徽省博士后基金项目（2019B320）作者简介：马李广（2000-），男，广东河源人，学士，研究方向为蔬菜种质资源与遗传育种。(E-mail)1554727563@qq.com。张贺龙为共同第一作者。通讯作者：陈国户，(E-mail)cgh@ahau.edu.cn
更新日期/Last Update: 2022-07-07