[1]张瑶,高弢,马桂珍,等.基于转录组测序技术分析愈创木酚对禾谷镰刀菌的抑菌机制[J].江苏农业学报,2022,38(02):343-351.[doi:doi:10.3969/j.issn.1000-4440.2022.02.007]
　ZHANG Yao,GAO Tao,MA Gui-zhen,et al.Analysis on the antifungal mechanism of Fusarium graminearum treated by guaiacol based on transcriptome sequencing[J].,2022,38(02):343-351.[doi:doi:10.3969/j.issn.1000-4440.2022.02.007]
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基于转录组测序技术分析愈创木酚对禾谷镰刀菌的抑菌机制()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
38
期数:
2022年02期
页码:
343-351
栏目:
植物保护
出版日期:
2022-04-30

文章信息/Info

Title:
Analysis on the antifungal mechanism of Fusarium graminearum treated by guaiacol based on transcriptome sequencing
作者:
张瑶1;  高弢2;  马桂珍1;  史建荣2
(1.江苏海洋大学,江苏连云港222000;2.江苏省农业科学院农产品质量安全与营养研究所/农业农村部农产品质量安全控制技术与标准重点实验室/农业农村部农产品质量安全风险评估实验室<南京>/江苏省食品质量安全重点实验室,江苏南京210014)
Author(s):
ZHANG Yao1; GAO Tao2; MA Gui-zhen1; SHI Jian-rong2
[1.Jiangsu Ocean University, Lianyungang 222000, China;2.Institute of Food Safety and Nutrition, Jiangsu Academy of Agricultural Sciences/Key Laboratory of Control Technology and Standard for Agro-Products Safety and Quality, Ministry of Agriculture and Rural Affairs/Key Lab of Agro-product Safety Risk Evaluation(Nanjing), Ministry of Agriculture and Rural Affairs/Key Laboratory of Food Quality and Safety, Nanjing 210014, China]
关键词:
禾谷镰刀菌; 愈创木酚; 转录组; 抑菌机制
Keywords:
Fusarium graminearum; guaiacol; transcriptome; antifungal mechanism
分类号:
Q946.887
DOI:
doi:10.3969/j.issn.1000-4440.2022.02.007
文献标志码:
A
摘要:
植物源酚类化合物愈创木酚可以显著抑制禾谷镰刀菌的生长，本研究利用转录组测序分析愈创木酚对禾谷镰刀菌的作用机制，采用RNA-Seq技术从转录组水平分析禾谷镰刀菌在0.4 μl/ml愈创木酚胁迫下的响应机制。结果显示，共筛选到905个差异表达基因表达量发生了变化，其中表达量上调的基因为464个，表达量下调的基因为441个。差异表达基因的COG、GO及Pathway功能分析发现，愈创木酚影响禾谷镰刀菌氧化应激反应、膜组分及离子运输途径。表明，愈创木酚处理后，禾谷镰刀菌的细胞膜完整性受到了破坏，Ca2+运输途径受到破坏，同时愈创木酚作为抗氧化剂也能显著影响禾谷镰刀菌的氧化应激反应。
Abstract:
The plant derived phenolic compound guaiacol can significantly inhibit the growth of Fusarium graminearum. In this study, the action mechanism of guaiacol on F. graminearum was analyzed by transcriptome sequencing, and the response mechanism of F. graminearum under 0.4 μl/ml guaiacol stress was analyzed by RNA-Seq technique at transcriptome level. The results showed that, 905 differentially expressed genes (DEGs) were screened to be varied in expression quantity, of which 464 were up-regulated and 441 were down-regulated. Analysis on the function of COG, GO and pathway of DEGs showed that, guaiacol affected oxidative stress response, membrane components and ion transport pathways of F. graminearum. In conclusion, the integrity of the cell membrane and Ca2+ transport pathway of F. graminearum were damaged after treated with guaiacol, and guaiacol could also significantly affect the oxidative stress response of F. graminearum as an antioxidant.

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备注/Memo

备注/Memo:
收稿日期：2021-05-26基金项目：国家重点研发计划项目（2018YFE0206000）；国家自然科学基金项目（31901936）作者简介：张瑶 (1997-)，女，江苏连云港人，硕士研究生，主要从事食品加工与安全方面的研究。（E-mail）1912682939@qq.com通讯作者：马桂珍， (E-mail)guizhenma@sohu.com；史建荣， （Tel）025-84392001,(E-mail)shiji@ jaas.ac.cn
更新日期/Last Update: 2022-05-07