[1]赵友学,杨燕波,朱宇峰,等.内生细菌Pantoea alhagi NX-11及其胞外多糖对低温胁迫下水稻苗的促生效应[J].江苏农业学报,2022,38(02):296-303.[doi:doi:10.3969/j.issn.1000-4440.2022.02.002]
　ZHAO You-xue,YANG Yan-bo,ZHU Yu-feng,et al.Effects of endophytic bacterium Pantoea alhagi NX-11 and its extracellular polysaccharides on the growth of paddy rice seedlings under low temperature stress[J].,2022,38(02):296-303.[doi:doi:10.3969/j.issn.1000-4440.2022.02.002]
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内生细菌Pantoea alhagi NX-11及其胞外多糖对低温胁迫下水稻苗的促生效应()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
38
期数:
2022年02期
页码:
296-303
栏目:
遗传育种·生理生化
出版日期:
2022-04-30

文章信息/Info

Title:
Effects of endophytic bacterium Pantoea alhagi NX-11 and its extracellular polysaccharides on the growth of paddy rice seedlings under low temperature stress
作者:
赵友学1; 杨燕波2; 朱宇峰1; 张国庆1; 薛听听1; 孙良1; 雷鹏1; 2; 徐虹1; 冯小海2; 许宗奇1; 2
（1.南京工业大学食品与轻工学院，江苏南京211816；2.南京轩凯生物科技股份有限公司,江苏南京210009）
Author(s):
ZHAO You-xue1; YANG Yan-bo2; ZHU Yu-feng1; ZHANG Guo-qing1; XUE Ting-ting1; SUN Liang1; LEI Peng1; 2; XU Hong1; FENG Xiao-hai2; XU Zong-qi1; 2
（1.College of Food and Light Industry, Nanjing Tech University, Nanjing 211816, China;2.Nanjing Xuankai Biotechnology Co., Ltd., Nanjing 210009, China）
关键词:
Pantoea alhagi; 水稻苗; 胞外多糖; 低温胁迫; 低温耐受性
Keywords:
Pantoea alhagi; rice seedling; extracellular polysaccharide; low temperature stress; low temperature tolerance
分类号:
S432.4+2
DOI:
doi:10.3969/j.issn.1000-4440.2022.02.002
文献标志码:
A
摘要:
以1株高产胞外多糖的内生细菌Pantoea alhagi NX-11为研究对象，通过构建的多糖分泌缺失菌株NX-11eps-以及分离到的胞外多糖（EPS）纯品，研究菌株NX-11及其胞外多糖对低温胁迫下水稻苗的促生效应。结果表明，菌株NX-11显著缓解了低温胁迫导致的水稻苗茎长、根长、鲜质量、干质量和相对含水量的降低。对其作用机制研究发现，NX-11通过诱导水稻苗内抗氧化酶活性提高显著降低了低温胁迫对水稻苗的氧化伤害；通过增强水稻苗脂肪酸去饱和酶（FADS）活性，提高了膜脂的不饱和度和双键指数，增强了膜脂流动性；通过影响水稻苗的激素合成，进而增强水稻苗在低温胁迫环境下的调控能力，增强其低温耐受性。与NX-11相比，低温胁迫下，多糖分泌缺失的菌株NX-11eps-对水稻苗的促生效应降低，而胞外多糖纯品表现出与NX-11一致的促生效果，表明NX-11胞外多糖在增强水稻苗低温耐受性的过程中发挥了关键作用。
Abstract:
In this study, the endophytic bacterium Pantoea alhagi NX-11 with high extracellular polysaccharide production was used as the research object. The growth-promoting effects of strain NX-11 and its extracellular polysaccharides on rice seedlings under low temperature stress were studied by constructing polysaccharide secretion deficient strain NX-11eps- and adding the isolated pure extracellular polysaccharide (EPS). The results showed that strain NX-11 significantly relieved the decrease of stem length, root length, fresh weight, dry weight and relative water content of rice seedlings caused by low temperature stress. The research on the action mechanism showed that, NX-11 significantly reduced the oxidative damage to rice seedlings under low temperature stress by inducing the increase of antioxidant enzyme activities in rice seedlings. The unsaturation degree and double bond index of membrane lipids were improved, and the fluidity of the membrane lipids was increased by enhancing the activity of the fattyacid desaturases （FADS） of the rice seedlings. The hormone synthesis of the rice seedlings was affected to enhance their regulation ability under the environment of low temperature stress, and to enhance their tolerance of low temperature. Compared with NX-11, under low temperature stress, the growth-promoting effect of polysaccharide secretion deficient strain NX-11eps- to rice seedlings was reduced, while the pure exopolysaccharide showed the same growth-promoting effect as NX-11, suggesting that exopolysaccharide of NX-11 played a key role in enhancing the low temperature tolerance of rice seedlings.

参考文献/References:

[1]YOSHIDA S. Fundamentals of rice crop science[M].Laguna：Philippines，1981.
[2]丁旗. 低温天气对水稻育苗的影响及综合预防[J].吉林农业, 2017（15）: 99.
[3]孙韵雅,陈佳,王悦,等. 根际促生菌促生机理及其增强植物抗逆性研究进展[J].草地学报,2020,28(5):1203-1215.
[4]姜焕焕,王通,陈娜,等. 根际促生菌提高植物抗盐碱性的研究进展[J].生物技术通报,2019,35(10):189-197.
[5]纪超,王晓辉,刘训理. 盐胁迫环境下植物促生菌的作用机制研究进展[J].生物技术通报,2020,36(4):131-143.
[6]SUBRAMANIAN P, KRISHNAMOORTHY R, CHANRATANA M. Expression of an exogenous 1-aminocyclopropane-1-carboxylate deaminase gene in psychrotolerant bacteria modulates ethylene metabolism and cold induced genes in tomato under chilling stress[J].Plant Physiol Biochem，2015,89:18-23.
[7]TURAN M, GULLUCE M, CAKMAK R, et al. Effect of plant growth-promoting rhizobacteria strain on freezing injury and antioxidant enzyme activity of wheat and barley[J].J Plant Nutr，2013, 36:731-748.
[8]ALAMI Y, ACHOUAK W, MAROL C, et al. Rhizosphere soil aggregation and plant growth promotion of sunflowers by an exopolysaccharide-producing Rhizobium sp. strain isolated from sunflower roots[J].Appl Environ Microbiol，2000,66, 3393-3398.
[9]ASHRAF M, HASNAIN S, BERGE O, et al. Inoculating wheat seedlings with exopolysaccharide-producing bacteria restricts sodium uptake and stimulates plant growth under salt stress[J].Biol Fertil Soils，2004, 40, 157-162.
[10]SUN L, YANG Y, LEI P, et al. Structure characterization, antioxidant and emulsifying capacities of exopolysaccharide derived from Pantoea alhagi NX-11[J].Carbohydrate Polymers, 2021, 261:117872.
[11]SUN L, YANG Y, WANG R, et al. Effects of exopolysaccharide derived from Pantoea alhagi NX-11 on drought resistance of rice and its efficient fermentation preparation[J].International Journal of Biological Macromolecules, 2020, 162(9)：46-55.
[12]赵海新. 碱胁迫对水稻叶绿素及叶片脯氨酸和可溶性糖含量的影响[J].作物杂志, 2020(1):98-102.
[13]李仕友. 植物富集铀镉特征及其响应机制试验研究[M]. 衡阳：南华大学,2019.
[14]XIA X J, WANG Y J, ZHOU Y H, et al. Reactive oxygen species are involved in brassinosteroid-induced stress tolerance in cucumber[J]. Plant Physiology, 2009, 150(2): 801-814.
[15]DROGE W. Free radicals in the physiological control of cell function[J].Physiological Reviews, 2002,82：47-95.
[16]PENG L, XU Z Q, DING Y, et al. Effect of poly(γ-glutamic acid) on the physiological responses and calcium signaling of rape seedlings(Brassica napus L.) under cold stress[J].J Agr Food Chem，2015,63:10399-10406.
[17]UR I, SKOCZOWSKI A, NIEMCZYK E, et al. Changes in the composition of fatty acids and sterols of membrane lipids during induction and differentiation of Brassica napus (var. oleifera L.) callus [J]. Acta Physiologiae Plantarum, 2002, 24(1): 3-10.
[18]BALTRUSCHAT H, FODOR J, HARRACH B D, et al. Salt tolerance of barley induced by the root endophyte Piriformospora indica is associated with a strong increase in antioxidants [J]. The New Phytologist, 2008, 180(2): 501-510.
[19]千春录，朱芹，高姗，等. 外源褪黑素处理对采后水蜜桃冷藏品质及冷害发生的影响[J].江苏农业学报，2020,36(3):702-708.
[20]马娟娟，赵斌，陈颖，等. 4个北美冬青品种苗对低温胁迫的生理响应及抗寒性比较[J].南京林业大学学报（自然科学版），2020,44（5）：34-40.
[21]张典利,孟臻,亓文哲,等. 植物根际促生菌的研究与应用现状[J].世界农药,2018,40(6):37-43，50.
[22]麦靖雯,黎瑞君,张巨明. 植物根际促生菌研究综述[J].现代农业科技,2018(12):179-180，183.
[23]官宇，余伟，辜运富，等. PGPR菌剂对砷胁迫下蜈蚣草根际微生物群落的影响[J].江苏农业科学，2020，48（19）：275-280.
[24]韦江璐，覃英，谢显秋，等. 促生菌对土壤养分、酶活性及细菌群落功能多样性的影响[J].南方农业学报，2020， 51（10）:2348-2357.
[25]纪超,王晓辉,刘训理. 盐胁迫环境下植物促生菌的作用机制研究进展[J].生物技术通报,2020,36(4):131-143.
[26]UPADHYAY S K, SINGH J S, SINGH D P,et al. Exopolysaccharide-producing plant growth-promoting rhizobacteria under salinity condition[J]. Pedosphere，2011, 21：214-222.
[27]NANDAL K, SEHRAWAT A R, YADAV A S, et al. High temperature-induced changes in exopolysaccharides, lipopolysaccharides and protein profile of heat-resistant mutants of Rhizobium sp. ( Cajanus )[J]. Microbiological Research,2005,160(4)：367-373.
[28]PULSAWAT W,LEKSAWASDI P, ROGERS L, et al. Anions effects on biosorption of Mn(Ⅱ) by extracellular polymeric substance (EPS) from Rhizobium etli[J]. Biotechnology Letters,2003,25(15):1267-1270.
[29]KASOTIA A, VARMA A, TUTEJA N, et al. Amelioration of soybean plant from saline-induced condition by exopolysaccharide producing Pseudomonas-mediated expression of high affinity K+ transporter (HKT1) gene[J]. Current Science,2016,111(12)：1961-1967.
[30]KHAN N, BANO A. Exopolysaccharide producing rhizobacteria and their impact on growth and drought tolerance of wheat grown under rainfed conditions[J]. PLoS One,2019,14(9)：1267-1270.
[31]MISHRA P K, BISHT S C, RUWARI P, et al. Alleviation of cold stress in inoculated wheat (Triticum aestivum L.) seedlings with psychrotolerant Pseudomonads from NW Himalayas[J]. Archives of Microbiology,2011,193(7)：497-513.
[32]ZUBAIR M, HANIF A, FARZAND A, et al. Genetic screening and expression analysis of psychrophilic Bacillus spp. reveal their potential to alleviate cold stress and modulate phytohormones in wheat[J]. Microorganisms,2019,7(9)：337.
[33]UPADHYAY S K, SINGH J S, SINGH D P. Exopolysaccharide-producing plant growth-promoting rhizobacteria under salinity Condition[J]. Pedosphere,2011,21(2):214-222.
[34]ASHRAF, M, HASNAIN S, BERGE O, et al. Inoculating wheat seedlings with exopolysaccharide-producing bacteria restricts sodium uptake and stimulates plant growth under salt stress[J]. Biology and Fertility of Soils,2004,40(3)：157-162.

备注/Memo

备注/Memo:
收稿日期：2021-07-28基金项目：国家自然科学基金青年科学基金项目（3180011491）；江苏省自然科学基金青年科学基金项目（BK20180158）；国家级大学生创新创业计划项目（202110291010Z）作者简介：赵友学（1999-），山东聊城人，本科，研究方向为根际促生微生物对作物的促生抗逆作用机制。（E-mail）201821019117@njtech.edu.cn通讯作者：雷鹏，（E-mail）lei-peng@njtech.edu.cn
更新日期/Last Update: 2022-05-07