[1]徐鹿,罗光华,金瑜剑,等.转录组测序分析毒死蜱与醚菊酯混配对二化螟毒杀的增效机制[J].江苏农业学报,2021,(02):317-325.[doi:doi:10.3969/j.issn.1000-4440.2021.02.006]
　XU Lu,LUO Guang-hua,JIN Yu-jian,et al.Study on the synergistic mechanism of chlorpyrifos and ethofenprox mixture in poisoning Chilo suppressalis based on transcriptome sequencing[J].,2021,(02):317-325.[doi:doi:10.3969/j.issn.1000-4440.2021.02.006]
点击复制

转录组测序分析毒死蜱与醚菊酯混配对二化螟毒杀的增效机制()
分享到：

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2021年02期
页码:
317-325
栏目:
植物保护
出版日期:
2021-04-30

文章信息/Info

Title:
Study on the synergistic mechanism of chlorpyrifos and ethofenprox mixture in poisoning Chilo suppressalis based on transcriptome sequencing
作者:
徐鹿1; 罗光华1; 金瑜剑2; 徐德进1; 徐广春1; 顾中言1
（1.江苏省农业科学院植物保护研究所，江苏南京210014；2.上海祥音生物科技有限公司，上海201800）
Author(s):
XU Lu1; LUO Guang-hua1; JIN Yu-jian2; XU De-jin1; XU Guang-chun1; GU Zhong-yan1
(1.Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;2.Shanghai Xiangyin Biotechnology Co., Ltd., Shanghai 201800, China)
关键词:
二化螟; 毒死蜱; 醚菊酯; 转录组测序; 增效
Keywords:
Chilo suppressalis; chlorpyrifos; ethofenprox; transcriptome sequencing; synergism
分类号:
S482.3;S481
DOI:
doi:10.3969/j.issn.1000-4440.2021.02.006
文献标志码:
A
摘要:
为明确毒死蜱与醚菊酯混配对二化螟毒杀的增效机制，采用联合毒力指数法筛选毒死蜱与醚菊酯混配的增效比例，利用转录组测序分析毒死蜱与醚菊酯混配的增效机制。结果表明，毒死蜱与醚菊酯以1∶2（质量比）混配时表现出增效作用，Illumina HiSeqTM X Ten测序获得高质量的二化螟转录组。去离子水处理与毒死蜱处理、去离子水处理与醚菊酯处理、去离子水处理与增效混配剂处理、毒死蜱处理与增效混配剂处理、醚菊酯处理与增效混配剂处理的差异表达基因分别为465、392、1 874、117、25个。对毒死蜱与醚菊酯混配增效主要涉及的代谢过程进行通路分析，结合维恩交集法进行分析，发现解毒代谢酶可能介导毒死蜱与醚菊酯混配的增效机制。实时荧光定量PCR结果证实差异基因的表达量与测序数据匹配。本研究结果可为解析杀虫剂混配增效机制提供分子基础，为优化杀虫剂混配比例，建立二化螟抗性治理策略提供理论依据。
Abstract:
To reveal the synergistic mechanism of chlorpyrifos and ethofenprox mixture in poisoning Chilo suppressalis, the synergistic ratios of chlorpyrifos and ethofenprox mixture were screened by combined toxicity index method, the synergistic mechanism of chlorpyrifos and ethofenprox mixture were analyzed by transcriptome sequencing. The results indicated that chlorpyrifos and ethofenprox mixed at a 1∶2 mass ratio showed significant synergism on C. suppressalis. HiSeqTM X Ten platform was used to obtain C. suppressalis transcriptome with high quality data. 465, 392, 1 874, 117 and 25 differentially expressed genes were obtained between the groups of deionized water and chlorpyrifos, deionized water and ethofenprox, deionized water and synergistic mixture, chlorpyrifos and synergistic mixture, ethofenprox and synergistic mixture. Pathway analysis showed that synergistic effect of chlorpyrifos and ethofenprox mixture was mainly involved in the metabolic process, and it was speculated that detoxification metabolic enzymes might mediate synergism mechanism of chlorpyrifos and ethofenprox combined with Venn intersection algorithm. The expression of significant different genes was highly matched with transcriptome sequencing data by quantitative real-time polymerase chain reaction (PCR). The results can provide a molecular basis for analyzing synergism mechanism of insecticide mixtures, and provide a theoretical basis for optimizing ratio of insecticide mixtures and establishing resistance control strategy of C. suppressalis.

参考文献/References:

[1]顾中言，许小龙，徐德进，等. 杀虫剂混合使用的增效作用评判分析[J]. 农药学学报，2009，11(3)：304-311.
[2]TONG S M, FENG M G. A mixture of putative sodium salts of camptothecin and bamboo tar is a novel botanical insecticide against rice planthoppers and stem borers [J]. Journal of Pest Science, 2016, 89: 1003-1011.
[3]PERRE C D, MURPHY T M, LYDY M J. Mixture toxicity of phostebupirim and cyfluthrin: species-specific responses [J]. Environmental Toxicology and Chemistry, 2017, 36(7): 1947-1954.
[4]YU Y Y, TING L C. Synergistic effect and field control efficacy of the binary mixture of permethrin and chlorpyrifos to brown planthopper (Nilaparvata lugens) [J]. Journal of Asia-Pacific Entomology, 2019, 22(1): 67-76.
[5]ATTIQUE M, KHALIQ A, SAYYED A. Could resistance to insecticides in Plutella xylostella (Lep., Plutellidae) be overcome by insecticide mixtures?[J]. Journal of Applied Entomology, 2006, 130（2）: 122-127.
[6]余海明，魏方林，朱国念. 三唑磷与氟虫腈复配对粘虫的增效机理研究[J]. 农药学学报，2009，11(3)：317-322.
[7]XU L, LUO G H, SUN Y, et al. Multiple down-regulated cytochrome P450 monooxygenase genes contributed to synergistic interaction between chlorpyrifos and imidacloprid against Nilaparvata lugens [J]. Journal of Asia Pacific Entomology, 2020, 23: 44-50.
[8]JIANG W H, JIANG X J, YE J R, et al. Rice striped stem borer, Chilo suppressalis (Lepidoptera: Pyralidae), overwintering in super rice and its control using cultivation techniques [J]. Crop Protection, 2011, 30(2): 130-133.
[9]姜玉英，刘万才，黄冲，等. 2020年全国农作物重大病虫害发生趋势预报[J]. 中国植保导刊，2020，40(2)：37-53.
[10]XU L, ZHAO J, SUN Y, et al. Constitutive overexpression of cytochrome P450 monooxygenase genes contributes to chlorantraniliprole resistance in Chilo suppressalis (Walker) [J]. Pest Management Science, 2019,75(3):718-725.
[11]韩平. 二化螟对醚菊酯的抗性及相关代谢酶基因的研究[D]. 南京：南京农业大学, 2014.
[12]WANG B J, WANG Y, ZHANG Y, et al. Genome-wide analysis of esterase-like genes in the striped rice stem borer, Chilo suppressalis [J]. Genome, 2015, 58(6): 323-331.
[13]CHOU T C, TALALAY P. Quantitative analysis of dose-effect relationships: the combined effects of multiple drugs or enzyme inhibitors [J]. Advances in Enzyme Regulation, 1984, 22: 27-55.
[14]SHI X Q, XIONG M H, JIANG W H, et al. Efficacy of endosulfan and fipronil and joint toxic action of endosulfan mixtures against Leptinotarsa decemlineata (Say) [J]. Journal of Pest Science, 2012, 85: 519-526.
[15]姜卫华. 二化螟的抗药性及综合防治研究[D]. 南京：南京农业大学, 2011.
[16]YAO R, ZHAO D D, ZHANG S, et al. Monitoring and mechanisms of insecticide resistance in Chilo suppressalis (Lepidoptera: Crambidae), with special reference to diamides [J]. Pest Management Science, 2017, 73: 1169-1178.
[17]LI X X, LI R, ZHU B, et al. Overexpression of cytochrome P450 CYP6BG1 may contribute to chlorantraniliprole resistance in Plutella xylostella (L.) [J]. Pest Management Science, 2018, 74(6): 1386-1393.
[18]MENG X, DONG F, KUN Q, et al. Transcriptome analysis reveals global gene expression changes of Chilo suppressalis in response to sublethal dose of chlorantraniliprole [J]. Chemosphere, 2019, 234: 648-657.
[19]MA W H, ZHAO X X, YIN C L, et al. A chromosome level genome assembly reveals the genetic basis of cold tolerance in a notorious rice insect pest, Chilo suppressalis [J]. Molecular Ecology Resources, 2020, 20: 268-282.
[20]周大颜，张志新，黄彩林，等. 3种白鲫杂交子代的转录组学分析[J]. 南方农业学报，2019,50(6):1328-1338.
[21]潘宝贵，钱恒彦，戈伟，等．辣椒应答冷信号转导机制研究进展[J]．江苏农业学报，2019，35(3)：743-748.
[22]ELZAKI M E A, MIAH M A, PENG Y C, et al. Deltamethrin is metabolized by CYP6FU1, a cytochrome P450 associated with pyrethroid resistance in Laodelphax striatellus [J]. Pest Management Science, 2018, 74: 1265-1271.
[23]WANG H D, SHI Y, WANG L, et al. CYP6AE gene cluster knockout in Helicoverpa armigera reveals role in detoxification of phytochemicals and insecticides [J]. Nature Communications, 2018, 9: 4820.

相似文献/References:

[1]贺江,龙海波.分散液相微萃取-高效液相色谱法测定水体中毒死蜱残留[J].江苏农业学报,2015,(05):1179.[doi:doi:10.3969/j.issn.1000-4440.2015.05.037]
　HE Jiang,LONG Hai-bo.A dispersive liquid-liquid microextraction-HPLC method for determination of chlorpyrifos in water[J].,2015,(02):1179.[doi:doi:10.3969/j.issn.1000-4440.2015.05.037]
[2]胡代花.中国水稻二化螟性信息素研究及应用新进展[J].江苏农业学报,2019,(03):736.[doi:doi:10.3969/j.issn.1000-4440.2019.03.033]
　HU Dai-hua.Recent advances in the research and application of sex pheromone of Chilo suppressalis(Walker) in China[J].,2019,(02):736.[doi:doi:10.3969/j.issn.1000-4440.2019.03.033]
[3]董成,冯发运,马丽雅,等.生物炭固定化菌剂对毒死蜱污染土壤的修复及小白菜品质的改善[J].江苏农业学报,2024,(05):846.[doi:doi:10.3969/j.issn.1000-4440.2024.05.009]
　DONG Cheng,FENG Fayun,MA Liya,et al.Remediation of chlorpyrifos contaminated soil by biochar immobilized bacteria and improvement of Chinese cabbage quality[J].,2024,(02):846.[doi:doi:10.3969/j.issn.1000-4440.2024.05.009]

备注/Memo

备注/Memo:
收稿日期：2020-07-11基金项目：国家自然科学基金项目（31972309、31672024）；国家重点研发计划项目（2017YFD0200305）；江苏省农业科技自主创新基金项目[CX(19)3114]作者简介：徐鹿（1982-），男，山东淄博人，博士，副研究员，主要从事农药毒理学研究。(E-mail)xulupesticide@163.com
更新日期/Last Update: 2021-05-10