[1]徐运林,房浩,周柏宇,等.甜玉米种质资源种子性状全基因组关联分析[J].江苏农业学报,2021,(02):289-295.[doi:doi:10.3969/j.issn.1000-4440.2021.02.003]
　XU Yun-lin,FANG Hao,ZHOU Bo-yu,et al.Genome-wide association study of grain traits of sweet maize germplasm resources[J].,2021,(02):289-295.[doi:doi:10.3969/j.issn.1000-4440.2021.02.003]
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甜玉米种质资源种子性状全基因组关联分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2021年02期
页码:
289-295
栏目:
遗传育种·生理生化
出版日期:
2021-04-30

文章信息/Info

Title:
Genome-wide association study of grain traits of sweet maize germplasm resources
作者:
徐运林; 房浩; 周柏宇; 易月明; 王长进; 程昕昕; 余海兵
（安徽科技学院农学院，安徽凤阳233100）
Author(s):
XU Yun-lin; FANG Hao; ZHOU Bo-yu; YI Yue-ming; WANG Chang-jin; CHENG Xin-xin; YU Hai-bing
(College of Agriculture, Anhui Science and Technology University, Fengyang 233100, China)
关键词:
甜玉米; 籽粒性状; 单核苷酸多态性（SNP）; 全基因组关联分析（GWAS）
Keywords:
sweet maize; grain traits; single nucleotide polymorphism（SNP）; genome-wide association study（GWAS）
分类号:
S330;S513
DOI:
doi:10.3969/j.issn.1000-4440.2021.02.003
文献标志码:
A
摘要:
粒长、粒宽、粒厚、百粒质量是影响玉米籽粒产量的重要性状，本研究主要统计分析了100份甜玉米种质资源的粒长、粒宽、粒厚和百粒质量等4个种子性状，并利用37 297个单核苷酸多态性（SNP）标记位点关联分析了控制甜玉米种子性状的重要位点。结果表明，100份甜玉米种质资源种子性状具有丰富的遗传多样性，性状变异幅度比较大，其中粒长为4.65～10.43 mm，粒宽为5.13～9.42 mm，粒厚为2.04～7.50 mm，百粒质量为4.51～20.09 g；表型变异分析发现，百粒质量的变异系数最大，为32.60%，粒宽的变异系数最小，为10.61%，且4个籽粒性状的频率分布曲线基本符合正态分布。相关性分析结果表明，粒长、粒宽、粒厚与百粒质量呈极显著正相关，粒长与粒厚呈极显著负相关。全基因组关联分析共检测到控制4个种子性状的75个SNP标记位点，其中有17个SNP标记位点与粒长性状有关，24个SNP标记位点与粒宽性状有关，11个SNP标记位点与粒厚性状有关，23个SNP标记位点与百粒质量性状有关。
Abstract:
The grain length, grain width, grain thickness and hundred-grain weight are important traits affecting the yield of maize. Four characters of seeds such as grain length, grain width, grain thickness and hundred-grain weight of 100 germplasm resources of sweet maize were summarized and analyzed in this study, and 37 297 single nucleotide polymorphism (SNP) loci were used to make correlation analysis on the important loci controlling the grain traits of sweet maize. The results showed that the grain traits of 100 germplasm resources of sweet maize had abundant genetic diversity with a relatively large variation range. The grain length was 4.65-10.43 mm, the grain width was 5.13-9.42 mm, the grain thickness was 2.04-7.50 mm and the hundred-grain weight was 4.51-20.09 g. Analysis on the phenotypic variation revealed that, the variable coefficient of the hundred-grain weight was the highest, with a value of 32.60%, and the variable coefficient of the grain width was the lowest, with a value of 10.61%. The distribution curves of frequencies of four grain traits were basically conformed to normal distribution. Results of correlation analysis showed that, the grain length, grain width and grain thickness were positively correlated with the hundred-grain weight very significantly, the grain length was negatively correlated with the grain thickness very significantly. A total of 75 SNP loci controlling the traits of four seeds were detected through genome-wide association study. Among the 75 SNP loci, 17 SNP loci were related with the grain length, 24 SNP loci were related with the grain width, 11 SNP loci were related with the grain thickness and 23 SNP loci were related with the hundred-grain weight.

参考文献/References:

[1]李春雨.利用染色体片段代换系及关联群体鉴定甜玉米产量性状遗传位点[D].广州：华南农业大学,2018.
[2]CHENG X X,XIONG F, WANG C J, et al.Seed reserve utilization and hydrolytic enzyme activities in germinating seeds of sweet corn[J]. Pakistan Journal of Botany,2018, 50(1):111-116.
[3]张秀荣.花生关联分析群体构建及抗旱相关分子标记研究[D].泰安：山东农业大学,2017.
[4]唐富福. 水稻农艺和品质若干性状的全基因组初步关联分析[D]. 杭州：浙江大学, 2013.
[5]杨宁.玉米17个农艺性状的全基因组关联分析及Mo17和大刍草基因组组装[D]. 武汉：华中农业大学,2016.
[6]向星洁.基于候选基因标记的香菇重要农艺性状关联分析[D].武汉：华中农业大学,2015.
[7]李桂冬.小麦3个重要农艺性状的SSR标记全基因组关联分析[D].杨凌：西北农林科技大学,2015.
[8]侯青青,司丽珍,黄学辉,等. 水稻复杂性状研究的新途径:水稻重要农艺性状全基因组关联分析[J].生命科学,2016,28(10):1250-1257.
[9]BRAULIO J, SOTO C, FEMANDO W, et al. Assessing the agronomic potential of linseed genotypes by multivariate analyses and association mapping of agronomic traits[J].Euphytica,2014(5):35-49.
[10]WANG G, JEFFREY M, LEONARDJ Z, et al. Marker-trait association analysis of kernel hardness and related agronomic traits in a core collection of wheat lines[J].Molecular Breeding, 2014, 177-184.
[11]张绵. 西藏野生大麦干旱耐性基因等位多态性分析及相关基因的功能鉴定[D].杭州：浙江大学,2017.
[12]史亚兴,卢柏山,宋伟,等. 基于SNP标记技术的糯玉米种质遗传多样性分析[J]. 华北农学报, 2015,30(3):77-82.
[13]BEL A, ZHENG P, LUCK S, et al. Whole genome scan detects an allelic variant of fad2 associated with increased oleic acid levels in maize[J]. Mol Geneet Genomics, 2008, 279:1-10.
[14]YANG Q, LI Z, LI W Q, et al. CACTA-like transposable element in ZmCCT attenuated photoperiod sensitivity and accelerated the postdomestication spread of maize[J]. Proc Natl Acad Sci USA, 2013, 110:16969-16974.
[15]RASHID Z, SINGH P K, VEMURI H, et al. Genome-wide association study in Asia-adapted tropical maize reveals novel and explored genomic regions for sorghum downy mildew resistance[J].Scientific Reports, 2018(8):366.
[16]李用财. 东北中熟玉米杂交组合主要农艺性状鉴定[D].哈尔滨：黑龙江大学,2018.
[17]赵檀. 河北省小麦品种遗传多样性及主要农艺性状的关联分析[D].保定：河北农业大学,2014.
[18]么大轩，张彬，刘松涛，等．基于SNP和SSR对甜玉米种质遗传多样性的评价[J]. 江苏农业科学，2019，47(7)：45-50.
[19]FANG C, XING Y, MAO H, et al. GS3,a major QTL for grain length and weight and minor QTL for grain width and thickness in rice,encodes a putative transnenbrane protein[J].Theoretical & Applied Genetics,2006,112(6):1164-1171.
[20]HU G, ZHAO L, LU Y, et al. Genome wide association study identified multiple genetic loci on chilling resistance during germination in maize[J].Scientific Reports, 2017, 7(1):10840.

备注/Memo

备注/Memo:
收稿日期：2020-07-28基金项目：国家自然科学基金委员会应急管理项目（31440066）；安徽省高校自然科学研究重大项目（KJ2019ZD57）；安徽科技学院稳定人才项目作者简介：徐运林（1996-），女，安徽岳西人，硕士研究生，主要从事玉米遗传育种方面的研究。（E-mail）xuyunlin0825@163.com通讯作者：程昕昕，（E-mail）chengxin0901@163.com
更新日期/Last Update: 2021-05-10