[1]朱璐,李淑顺,闻婧,等.鸡爪槭ApMYB306基因克隆及功能分析[J].江苏农业学报,2020,(06):1512-1520.[doi:doi:10.3969/j.issn.1000-4440.2020.06.022]
　ZHU Lu,LI Shu-shun,WEN Jing,et al.Cloning and functional analysis of ApMYB306 gene in Acer palmatum[J].,2020,(06):1512-1520.[doi:doi:10.3969/j.issn.1000-4440.2020.06.022]
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鸡爪槭ApMYB306基因克隆及功能分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2020年06期
页码:
1512-1520
栏目:
园艺
出版日期:
2020-12-31

文章信息/Info

Title:
Cloning and functional analysis of ApMYB306 gene in Acer palmatum
作者:
朱璐1; 李淑顺1; 闻婧1; 马秋月1; 颜坤元1; 任杰2; 李倩中1
(1.江苏省农业科学院休闲农业研究所，江苏南京210014；2.安徽省农业科学院农业工程研究所，安徽合肥230031)
Author(s):
ZHU Lu1; LI Shu-shun1; WEN Jing1; MA Qiu-yue1; YAN Kun-yuan1; REN Jie2; LI Qian-zhong1
(1.Institute of Leisure Agriculture， Jiangsu Academy of Agricultural Sciences, Nanjing 210014， China;2.Institute of Agricultural Engineering, Anhui Academy of Agricultural Sciences, Hefei 230031, China)
关键词:
鸡爪槭; ApMYB306基因; 抗逆性
Keywords:
Acer palmatum; ApMYB306 gene; stress resistance
分类号:
S687
DOI:
doi:10.3969/j.issn.1000-4440.2020.06.022
文献标志码:
A
摘要:
采用基因克隆的方法获得鸡爪槭金陵黄枫R2R3-MYB转录因子基因，经同源比对和生物信息学分析将其命名为ApMYB306。通过组织表达特性分析发现ApMYB306在茎中表达量最高，叶中表达量次之，根中表达量最低。在洋葱表皮细胞中瞬时表达分析结果显示ApMYB306蛋白定位于细胞核中。ApMYB306对于高温、低温、干旱和盐胁迫均有明显的响应，受胁迫诱导后表达量显著上升。冷冻处理后，转ApMYB306基因株系成活率较野生型升高。ApMYB306超表达拟南芥株系较野生型开花延迟，表明ApMYB306可能通过抑制拟南芥FT基因启动子的表达调控花期。
Abstract:
In this study, the R2R3-MYB transcription factor gene was obtained from Acer palmatum Jinlinghuangfeng by gene cloning and named ApMYB306 via homology comparison and bioinformatics analysis. Through the analysis of tissue expression characteristics, it was found that the expression level of ApMYB306 was the highest in the stem, followed by leaves, and the expression level was lowest in the roots. The results of transient expression analysis in onion epidermal cells showed that ApMYB306 protein was localized in the nucleus. ApMYB306 had obvious response to high temperature, low temperature, drought and salt stress, and its expression level increased significantly after induction. After freezing treatment, the survival rate of ApMYB306 transgenic lines was higher than that of the wild-type lines. The flowering time of ApMYB306 overexpressing Arabidopsis lines was later than that of the wild-type lines, which indicated that ApMYB306 might regulate the flowering time by inhibiting the expression of the FT gene promoter.

参考文献/References:

［1］LI S S, LI Q Z, TANG L, et al. Pigment comparison and expression of chlorophyll metabolism genes in yellow and green Acer palmatum leaves[J]. Canadian Journal of Plant Science, 2017, 97: 1-21.
［2］DUBOS C, STRACKE R, GROTEWOLD E, et al. MYB transcription factors in Arabidopsis[J]. Trends in Plant Science, 2010, 15:573-581.
［3］STRACKE R, WERBER M, WEISSHAAR B, The R2R3-MYB gene family in Arabidopsis thaliana[J]. Current Opinion in Plant Biology, 2001, 4:447-456.
［4］YILMAZ A, NISHIYAMA M Y J, FUENTES B G, et al. GRASSIUS: a platform for comparative regulatory genomics across the grasses[J]. Plant Physiology, 2009, 149:171-180.
［5］CAO Z H, ZHANG S Z, WANG R K, et al. Genome wide analysis of the apple MYB transcription factor family allows the identification of MdoMYB121 gene confering abiotic stress tolerance in plants[J]. PLoS One, 2013, 8: e69955.
［6］ZHAO P P, LI Q, LI J, et al. Genome-wide identification and characterization of R2R3MYB family in Solanum lycopersicum[J]. Molecular Genetics and Genomics, 2014, 289:1183-1207.
［7］WILKINS O, NAHAL H, FOONG J, et al. Expansion and diversification of the Populus R2R3-MYB family of transcription factors[J]. Plant Physiology, 2009, 149:981-993.
［8］AN J P, LI R, QU F J, et al. R2R3-MYB transcription factor MdMYB23 is involved in the cold tolerance and proanthocyanidin accumulation in apple[J]. The Plant Journal, 2018, 96(3):562-577.
［9］AN Y, DAI X, ZHANG W H. A R2R3-type MYB gene, OsMYB2, is involved in salt, cold, and dehydration tolerance in rice[J]. Journal of Experimental Botany, 2012, 63(7):2541-2556.
［10］COMINELLI E, GALBIATI M, VAVASSEUR A, et al. A guard-cell-specific MYB transcription factor regulates stomatal movements and plant drought tolerance[J]. Current Biology, 2005, 15:1196-1200.
［11］OH J E, KWON Y, KIM J H, et al. A dual role for MYB60 in stomatal regulation and root growth of Arabidopsis thaliana under drought stress[J]. Plant Molecular Biology, 2011, 77:91-103.
［12］SEO P J, PARK C M. MYB96-mediated abscisic acid signals induce pathogen resistance response by promoting salicylic acid biosynthesis in Arabidopsis[J]. New Phytologist, 2010, 186:471-483.
［13］AGARWAL M, HAO Y, KAPOOR A, et al. A R2R3 type MYB transcription factor is involved in the cold regulation of CBF genes and in acquired freezing tolerance[J]. Journal of Biological Chemistry, 2006, 281:37636-37645.
［14］COMINELLI E, SALA T, CALVI D, et al. Over-expression of the Arabidopsis AtMYB41 gene alters cell expansion and leaf surface permeability[J]. The Plant Journal, 2008, 53:53-64.
［15］LIPPOLD F, SANCHEZ D H, MUSIALAK M, et al. AtMyb41 regulates transcriptional and metabolic responses to osmotic stress in Arabidopsis[J]. Plant Physiology, 2009, 149:1761-1772.
［16］REYES J L, CHUA N H. ABA induction of miR159 controls transcript levels of two MYB factors during Arabidopsis seed germination[J]. The Plant Journal, 2007, 49:592-606.
［17］ABE H,URA T, ITO T, et al. Arabidopsis AtMYC2 (bHLH) and AtMYB2 (MYB) function as transcriptional activators in abscisic acid signaling[J]. The Plant Cell, 2003, 15: 63-78.
［18］JUNG C, SEO J S, HAN S W, et al. Overexpression of AtMYB44 enhances stomatal closure to confer abiotic stress tolerance in transgenic Arabidopsis[J]. Plant Physiology, 2008, 146:623-635.
［19］SHAN H, CHEN S M, JIANG J F, et al. Heterologous expression of the chrysanthemum R2R3-MYB transcription factor CmMYB2 enhances drought and salinity tolerance, increases hypersensitivity to ABA and delays flowering in Arabidopsis thaliana[J]. Molecular Biotechnology, 2012, 51:160-173.
［20］ZHU L, GUAN Y X, LIU Y N, et al. Regulation of flowering time in chrysanthemum by the R2R3 MYB transcription factor CmMYB2 is associated with changes in gibberellin metabolism[J]. Horticulture Research, 2020, 7:96.
［21］CURTIS M D, GROSSNIKLAUS UA. Gateway cloning vector set for high-throughput functional analysis of genes in planta[J]. Plant Physiol, 2003, 133:462-469.
［22］LARKIN M A, BLACKSHIELDS G, BROWN N P, et al. Clustal W and clustal X version 2.0[J]. Bioinformatics, 2007, 23:2947-2948.
［23］ZHU L, MA Q Y, LI S S, et al. Reference gene selection for quantitative real-time PCR analyses of acer palmatum under abiotic stress[J]. Phyton-International Journal of Experimental Botany, 2020, 89:385-403.
［24］SHEN H , HE X , POOVAIAH C R, et al. Functional characterization of the switchgrass (Panicum virgatum) R2R3-MYB transcription factor PvMYB4 for improvement of lignocellulosic feedstocks[J]. New Phytologist, 2011, 193(1):121-136.
［25］EL-KEREAMY A, BI Y M, RANATHUNGE K, et al. The rice R2R3-MYB transcription factor OsMYB55 is involved in the tolerance to high temperature and modulates amino acid metabolism[J]. PLoS One, 2012, 7: e52030.
［26］QIN Y X, WANG M C, TIAN Y C, et al. Over-expression of TaMYB33 encoding a novel wheat MYB transcription factor increases salt and drought tolerance in Arabidopsis[J]. Molecular Biology Reports, 2012, 39:7183-7192.
［27］申晴,殷亚蕊,武军凯,等. 桃MYB3基因的克隆及表达分析[J].河北果树, 2020(2):8-10,18,67.
［28］李红霞,汪妤,郭泾垒,等. 小麦热胁迫响应基因TaMYB165的克隆与功能分析[J].西北农林科技大学学报(自然科学版), 2019, 47(12):43-51.
［29］杨娟. 燕子花IlMYB306基因克隆及其抗逆功能初探[D].哈尔滨:东北林业大学, 2019.
[30]周凤珏，郑金元，龚银花，等. 诱蜂剂中蔗糖浓度对罗汉果花器官和坐果的影响[J]. 南方农业学报，2018,49(10):2007-2012.
[31]孙金秋，任相亮，胡红岩，等. 农田杂草群落演替的影响因素综述[J]. 杂草学报，2019，37(2)：1-9.
[32]杨为海，曾利珍，曾辉，等. 湛江地区澳洲坚果种质开花生物学特性观测分析[J]. 南方农业学报，2019,50(5):1042-1048.
[33]赵 薇，王爱花，陆慢，等．不同春化处理对蚕豆开花结荚时间和产量的影响[J]. 江苏农业科学，2018,46(16):102-105.
［34］MOUHU K, HYTONEN T, FOLTA K, et al. Identification of flowering genes in strawberry, a perennial SD plant[J]. BMC Plant Biology, 2009, 9:122.
［35］LIU S D, WANG X P, LI E Y, et al. R2R3 MYB transcription factor PtrMYB192 regulates flowering time in Arabidopsis by activating Flowering Locus C[J]. Journal of Plant Biology, 2013, 56:243-250.
［36］LIU L Y, ZHANG J, ADRIAN J, et al. Elevated Levels of MYB30 in the phloem accelerate flowering in Arabidopsis through the regulation of flowering locus T[J]. PLoS One, 2014, 9: e89799.

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备注/Memo

备注/Memo:
收稿日期：2020-09-07基金项目：国家自然科学基金项目(31700628)；江苏省自然科学基金项目(BK20170602)；江苏省农业科技自主创新基金项目[CX(17)1004]作者简介：朱璐(1987-)，女，山东淄博人，博士，助理研究员，研究方向为鸡爪槭遗传育种。(E-mail)luzhu@jaas.ac.cn通讯作者：李倩中，(E-mail)qianzhongli@jaas.ac.cn
更新日期/Last Update: 2021-01-15