[1]于少帅,赵文霞,姚艳霞,等.新疆野苹果和栽培苹果遗传分化与变异特征[J].江苏农业学报,2020,(05):1274-1281.[doi:doi:10.3969/j.issn.1000-4440.2020.05.026]
　YU Shao-shuai,ZHAO Wen-xia,YAO Yan-xia,et al.Genetic differentiation and variation characteristics of Malus sieversii and cultivated apples[J].,2020,(05):1274-1281.[doi:doi:10.3969/j.issn.1000-4440.2020.05.026]
点击复制

新疆野苹果和栽培苹果遗传分化与变异特征()
分享到：

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2020年05期
页码:
1274-1281
栏目:
园艺
出版日期:
2020-10-31

文章信息/Info

Title:
Genetic differentiation and variation characteristics of Malus sieversii and cultivated apples
作者:
于少帅1; 赵文霞2; 姚艳霞2; 张学超3; 淮稳霞2
（1.中国热带农业科学院椰子研究所,海南文昌571339;2.中国林业科学研究院森林生态环境与保护研究所/国家林业和草原局森林保护学重点实验室,北京100091;3.伊犁哈萨克自治州农业科学研究所,新疆伊宁835000）
Author(s):
YU Shao-shuai1; ZHAO Wen-xia2; YAO Yan-xia2; ZHANG Xue-chao3; HUAI Wen-xia2
(1.Coconut Research Institute of Chinese Academy of Tropical Agricultural Sciences, Wenchang 571339, China;2.Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry/Key Laboratory of Forest Protection, National Forestry and Grassland Administration, Beijing 100091, China;3.Institute of Agricultural Sciences of Ili Kazakh Autonomous Prefecture, Yining 835000, China)
关键词:
新疆野苹果; 微卫星标记; 遗传多样性; 系统发育; 抗逆性
Keywords:
Malus sieversii; microsatellite marker; genetic diversity; phylogeny; stress resistance
分类号:
S661.1
DOI:
doi:10.3969/j.issn.1000-4440.2020.05.026
文献标志码:
A
摘要:
利用15对特异性SSR引物对290份新疆野苹果和栽培苹果样品进行扩增，并通过分子生物学方法分析其遗传变异特征与系统发育关系。15对SSR引物在290份新疆野苹果和栽培苹果样品中共获得69个等位基因，平均每个SSR位点获得4.6个等位基因。新疆野苹果和栽培苹果亲缘关系较近，新疆野苹果种群遗传多样性丰富，遗传分化系数为0.25，基因流为0.76。新疆新源和巩留2个地区的新疆野苹果聚于一个进化分枝，栽培苹果与采自新疆的红肉苹果聚于一个进化分枝。编码转录因子RAX3的SSR位点在枯枝率大于90%的新疆野苹果样品中有特异条带；编码类TMV抗性蛋白质N端的SSR位点在新疆野苹果中有特异性条带；编码类TMV抗性蛋白质N端、转录因子bHLH74的SSR位点在栽培苹果中有特异性条带。
Abstract:
15 pairs of specific simple sequence repeats(SSR) primers were used to amplify 290 samples of Malus sieversii and cultivated apples, and their genetic variation and phylogenetic relationships were analyzed by molecular biology method. 69 alleles were obtained in 290 samples of M. sieversii and cultivated apples based on 15 pairs of SSR primers, with an average of 4.6 alleles per SSR locus. There was a close genetic relationship between M. sieversii and cultivated apples. The genetic diversity of M. sieversii groups was rich, with a genetic differentiation coefficient of 0.25 and a gene flow of 0.76. M. sieversii from Xinyuan and Gongliu of Xinjiang clustered in an evolutionary branch, cultivated apples and the red-flesh apple collected from Xinjiang clustered in an evolutionary branch. The SSR locus that coding transcription factor RAX3 showed a specific band in M. sieversii samples whose deadwood rate was above 90%. The SSR locus that coding the N end of the TMV-like resistant protein showed a specific band in M. sieversii samples. The SSR loci that coding the N end of the TMV-like resistant protein and the transcription factor bHLH74 showed specific bands in cultivated apples.

参考文献/References:

[1]束怀瑞. 苹果学[M]. 北京: 中国农业出版社, 1999.
[2]张艳敏,冯涛,张春雨,等. 新疆野苹果研究进展[J]. 园艺学报, 2009, 36(3): 447-452.
[3]刘华,臧润国,丁易,等. 天山西部新疆野苹果种群特征[J]. 林业科学, 2010, 46(11): 1-7.
[4]王春晓,赵褔,赵健桐,等. 新疆发生苹果小吉丁虫[J]. 新疆农业科学, 1995（5）: 225-226.
[5]刘爱华,张新平,温俊宝,等. 天山野苹果林苹果小吉丁与苹果树腐烂病复合危害研究[J]. 新疆农业科学, 2014, 51(12): 2240-2244.
[6]ZHANG C, CHEN X, HE T, et al. Genetic structure of Malus sieversii population from Xinjiang, China, revealed by SSR markers[J]. Journal of Genetics and Genomics, 2007, 34(10): 947-955.
[7]张春雨,陈学森,林群,等. 新疆野苹果群体遗传结构与遗传多样性的SRAP分析[J]. 园艺学报, 2009, 36(1): 7-14.
[8]DONG Y, ZHANG J, REN Y C, et al. Stduy on genetic diversity of natural population in Malus sieversii with microsatellite[J]. Journal of Plant Genetic Resources, 2013, 14(5): 771-777.
[9]OMASHEVA M, CHEKALIN S, GALIAKPAROV N. Evaluation of molecular genetic diversity of wild apple Malus sieversii populations from Zailiysky Alatau by microsatellite markers[J]. Russian Journal of Genetics, 2015, 51(7): 647-652.
[10]ZHOU Z Q, LI Y N. The RAPD evidence for the phylogenetic relationship of the closely related species of cultivated apple[J]. Genetic Resources and Crop Evolution, 2000, 47(4): 353-357.
[11]赵偑,杨美玲,龙鸿,等. 基于ITS序列的新疆野苹果系统发育分析[J]. 生物学杂志, 2013, 30(5): 19-22.
[12]CHE Y H, YANG Y P, YANG X M, et al. Phylogenetic relationship and diversity among Agropyron Gaertn germplasm using SSRs markers[J]. Plant Systematics and Evolution, 2015, 301(1): 163-170.
[13]GHARGHANI A, ZAMANI Z, TALAIE A, et al. Genetic identity and relationships of Iranian apple (Malus×domestica Borkh.) cultivars and landraces, wild Malus species and representative old apple cultivars based on simple sequence repeat (SSR) marker analysis[J]. Genetic Resources and Crop Evolution, 2009, 56(6): 829-842.
[14]SEGARRA-MORAGUES J G, IRIONDO J M, CATALAN P. Genetic fingerprinting of germplasm accessions as an aid for species conservation: a case study with Borderea chouardii (Dioscoreaceae), one of the most critically endangered Iberian plants[J]. Annals of Botany, 2005, 96 (7): 1283-1292.
[15]KLABUNDE G H F, JUNKES C F O, TENFEN S Z A, et al. Genetic diversity and apple leaf spot disease resistance characterization assessed by SSR markers[J]. Crop Breeding and Applied Biotechnology, 2016, 16(3). http://dx.doi.org/10.1590/1984-70332016v16n3a29.
[16]左力辉,张文林,邱彤,等. 新疆野苹果叶形性状变异及其与SSR标记关系分析[J]. 园艺学报, 2015, 42(4): 759-768.
[17]阿格里斯(美). 植物病理学[M]. 沈崇尧，译.北京: 中国农业大学出版社， 2009：3.
[18]VELASCO R, ZHARKIKH A, AFFOURTIT J, et al. The genome of the domesticated apple (Malus×domestica Borkh.)[J]. Nature Genetics, 2010, 42(10): 833-839.
[19]FREELAND J R, KIRK H, PETERSEN S D.分子生态学[M]. 戎俊,杨小强,耿宇鹏,等译.北京: 高等教育出版社, 2015：9.
[20]ZHANG H X, ZHANG M L, WANG L N. Genetic structure and historical demography of Malus sieversii in the Yili valley and the western mountains of the Junggar Basin, Xinjiang, China[J]. Journal of Arid Land, 2015, 7(2): 264-271.
[21]RICHARDS C M, VOLK G M, REILLEY A A, et al. Genetic diversity and population structure in Malus sieversii, a wild progenitor species of domesticated apple[J]. Tree Genetics and Genomes, 2009, 5(2): 339-347.
[22]MAGUIR T I, PEAKALL R, SAENGER P, et al. Comparative analysis of genetic diversity in the mangrove species Avicennia marina (Forsk.) Vierh. (Avicenniaceae) detected by AFLPs and SSRs[J]. Theoretical and Applied Genetics, 2002, 104: 388-398.
[23]梅闯,闫鹏,韩立群,等. 新疆野苹果不同类型单株对苹果小吉丁虫抗性差异[J]. 新疆农业科学, 2015, 52(10): 1859-1865.
[24]刘君,刁永强,陈淑英,等. 伊犁河谷不同苹果品种苗木冻害调查及分析[J]. 北方园艺, 2013(23): 34-37.

备注/Memo

备注/Memo:
收稿日期：2020-03-24基金项目：中央级公益性科研院所基本科研业务费专项(CAFYBB2017SZ003);国家重点研发计划项目(2016YFC0501503)作者简介：于少帅 (1987-),男,山东莱阳人,博士,助理研究员,主要从事分子植物病理学研究。 (E-mail)hzuyss@163.com通讯作者：姚艳霞,(E-mail)yaoyx@caf.ac.cn
更新日期/Last Update: 2020-11-16