[1]张柏林,刘宁,杨乾,等.20%菜籽粕和谷氨酰胺添加对黔东南小香鸡肠道指数及免疫机能的影响[J].江苏农业学报,2020,(05):1255-1264.[doi:doi:10.3969/j.issn.1000-4440.2020.05.024]
　ZHANG Bo-lin,LIU Ning,YANG Qian,et al.Effects of 20% rapeseed meal and glutamine supplementation on intestine indices and immune function of Qiandongnan Xiaoxiang chicken[J].,2020,(05):1255-1264.[doi:doi:10.3969/j.issn.1000-4440.2020.05.024]
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20%菜籽粕和谷氨酰胺添加对黔东南小香鸡肠道指数及免疫机能的影响()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2020年05期
页码:
1255-1264
栏目:
畜牧兽医·水产养殖
出版日期:
2020-10-31

文章信息/Info

Title:
Effects of 20% rapeseed meal and glutamine supplementation on intestine indices and immune function of Qiandongnan Xiaoxiang chicken
作者:
张柏林1; 刘宁1; 杨乾2; 谢宇潇1; 王庆容1; 宋培勇1; 曹珍1; 廖世永1
（1.遵义师范学院生物与农业科技学院赤水河流域特色动物资源保护与利用重点实验室，贵州遵义523006；2.吉林农业大学动物科技学院动物生产及产品质量安全教育部重点实验室，吉林长春130118）
Author(s):
ZHANG Bo-lin1; LIU Ning1; YANG Qian2; XIE Yu-xiao1; WANG Qing-rong1; SONG Pei-yong1; CAO Zhen1; LIAO Shi-yong1
（1.Department of Biology and Agriculture of Zunyi Normal College, Key Laboratory of Conservation and Utilization of Characteristic Animal Resources in Chishui River Basin, Zunyi 523006, China;2.College of Animal Science and Technology of Jilin Agricultural University, Jilin Provincial Key Laboratory of Animal Nutrition and Feed Science, Changchun 130118, China）
关键词:
谷氨酰胺; 菜籽粕; 黔东南小香鸡; 肠道指数; 免疫机能
Keywords:
glutamine; rapeseed meal; Qiandongnan Xiaoxiang chicken; intestine index; immune function
分类号:
S816.11
DOI:
doi:10.3969/j.issn.1000-4440.2020.05.024
文献标志码:
A
摘要:
为研究日粮中添加20%菜籽粕及20%菜籽粕日粮中添加不同水平谷氨酰胺（Glutamine，Gln）对黔东南小香鸡肠道指数及免疫机能的影响，将120只1 日龄体质量相近的黔东南小香鸡随机分为4组，分别为对照组（饲喂基础日粮）、菜籽粕组（菜籽粕等量替代基础日粮中20%豆粕）及菜籽粕组日粮中分别添加0.5%、1.0% Gln的试验组，每组5个重复，每个重复6只鸡，试验期为21 d。结果表明：与对照组相比，20%菜籽粕添加显著降低了采食量及体增质量（P<0.05），显著降低了十二指肠、空肠及回肠相对长度和相对质量（P<0.05）及脾脏、胸腺和法氏囊指数（P<0.05）。此外，20%菜籽粕添加还显著降低了血清免疫球蛋白A（Immunoglobulin A，IgA）、免疫球蛋白G（Immunoglobulin G, IgG）及免疫球蛋白M（Immunoglobulin M，IgM）含量（P<0.05），降低了空肠黏膜免疫分泌型免疫球蛋白A（Secretory immunoglobulin A，sIgA）含量以及空肠黏膜MUC1和MUC2 mRNA表达水平（P<0.05）。与20%菜籽粕组相比，20%菜籽粕日粮中添加0.5% Gln仅显著增加了回肠相对长度（P<0.05）；而20%菜籽粕日粮中添加1.0% Gln显著增加了十二指肠、空肠及回肠相对长度和相对质量（P<0.05），显著增加了脾脏、胸腺和法氏囊免疫器官指数（P<0.05），显著增加了血清IgA、IgG和IgM含量（P<0.05），显著增加了空肠黏膜sIgA含量和空肠黏膜MUC1和MUC2 mRNA表达水平（P<0.05）。由此可见，日粮中20%菜籽粕添加显著降低了黔东南小香鸡肠道指数及肠道免疫机能，但在20%菜籽粕日粮中添加1.0% Gln则对菜籽粕产生的抑制作用具有较好的改善作用。
Abstract:
The aim of this study was to investigate the effects of 20% rapeseed meal and 20% rapeseed meal supplemented with different levels of glutamine (Gln) on intestine indices and immune function of Qiandongnan Xiaoxiang chicken. A total of 120 1-day-old Qiandongnan Xiaoxiang chicken with similar body weight were randomly divided into four groups (A, B, C and D), and there were five replicates in each group and six chickens per replicate. Group A was defined as control group and was fed with the basal diet, whereas group B was fed with diets supplemented with 20% rapeseed meal (equivalent amount of soybean meal was replaced by rapeseed meal in the basal diet), and group C and group D were separately supplemented with 0.5% Gln and 1.0% Gln on the basis of the basal diet. The duration of this experiment was 21 days. Compared with the control group, dietary supplemented with 20% rapeseed meal significantly decreased feed intake and body weight gain （P<0.05）, decreased the relative length and relative weight of duodenum, jejunum and ileum （P<0.05） and decreased spleen index, thymus index and bursa index （P<0.05）. Moreover, dietary supplemented with 20% rapeseed meal significantly decreased the contents of immunoglobulin A(IgA), immunoglobulin G(IgG) and immunoglobulin M(IgM) in serum （P<0.05）, and decreased the secretory immunoglobulin A (sIgA) contents and mRNA expression levels of MUC1 and MUC2 in jejunal mucosa （P<0.05）. Compared with 20% rapeseed meal supplementation group, 0.5% Gln supplementation only increased relative length of ileum （P<0.05）, but 1.0% Gln supplementation significantly increased the relative length and relative weight of duodenum, jejunum and ileum and spleen index, thymus index and bursa index （P<0.05）. Additionally, 1.0% Gln supplementation increased the contents of IgA, IgG and IgM in serum （P<0.05） and the content of sIgA in jejunal mucosa （P<0.05）, and mRNA expression levels MUC1 and MUC2 in jejunal mucosa （P<0.05）. Therefore, 20% rapeseed meal supplementation significantly reduced intestine indices and immune function of Qiandongnan Xiaoxiang chicken, however, 1.0% Gln supplementation could improve the inhibitory effect.

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备注/Memo

备注/Memo:
收稿日期：2020-03-04基金项目：贵州省科技厅项目[黔科合基础2017(1205）]；贵州省高层次创新型人才项目[遵市科合人才(2017)10号]；遵义市校联合基金项目[遵市科合计(2018)08号]；遵义市“15851”人才项目（2050020213#）;贵州省教育厅项目[黔教合KY字(2016)258]；贵州省服务农村产业革命项目（遵师合农村产业字201905）作者简介：张柏林（1981-），男，山东烟台人，博士，副教授，研究方向为动物营养与生理调控。(E-mail)zhangbolin@zync.edu.cn
更新日期/Last Update: 2020-11-16