[1]王丽,杨晓阳,曹静,等.猪SUMO1基因的碱基序列特征及其在猪肺炎支原体感染过程中的表达[J].江苏农业学报,2020,(05):1229-1236.[doi:doi:10.3969/j.issn.1000-4440.2020.05.021]
　WANG Li,YANG Xiao-yang,CAO Jing,et al.Sequence characteristics of porcine SUMO1 gene and its expression during Mycoplasma hyopneumoniae infection[J].,2020,(05):1229-1236.[doi:doi:10.3969/j.issn.1000-4440.2020.05.021]
点击复制

猪SUMO1基因的碱基序列特征及其在猪肺炎支原体感染过程中的表达()
分享到：

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2020年05期
页码:
1229-1236
栏目:
畜牧兽医·水产养殖
出版日期:
2020-10-31

文章信息/Info

Title:
Sequence characteristics of porcine SUMO1 gene and its expression during Mycoplasma hyopneumoniae infection
作者:
王丽1; 2; 杨晓阳1; 2; 曹静3; 赵为民 1; 2; 涂枫1; 2; 付言峰1; 2; 李碧侠1; 2; 陈哲1; 2; 任守文1; 2; 方晓敏1; 2
(1.江苏省农业科学院畜牧研究所，江苏南京210014；2.农业部种养结合重点实验室，江苏南京210014；3.江苏省农业科学院江苏农科传媒有限公司，江苏南京210014)
Author(s):
WANG Li1; 2; YANG Xiao-yang1; 2; CAO Jing3; ZHAO Wei-min1; 2; TU Feng1; 2; FU Yan-feng1; 2; LI Bi-xia1; 2; CHEN Zhe1; 2; REN Shou-wen1; 2; FANG Xiao-min1; 2
(1.Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;2.Key Laboratory of Crop and Livestock Integration, Ministry of Agriculture, Nanjing 210014, China;3.Jiangsu Agricultural Science Media Co., Ltd., Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China)
关键词:
猪肺炎支原体; SUMO1; 猪肺泡巨噬细胞; 炎症反应
Keywords:
Mycoplasma hyopneumoniae; small ubiquitin-like modifier(SUMO1); porcine alveolar macrophages; inflammatory response
分类号:
S858.286.3
DOI:
doi:10.3969/j.issn.1000-4440.2020.05.021
文献标志码:
A
摘要:
根据NCBI 上GenBank 的猪类泛素蛋白修饰分子1(SUMO1)基因碱基序列(NM_001112676.1)，设计编码区全长上、下游引物，成功克隆了猪SUMO1基因完整的编码区序列。经生物信息学软件分析发现猪SUMO1基因的蛋白质编码区(CDS) 全长306 bp，编码101个氨基酸，SUMO1 的蛋白质氨基酸序列在物种间高度保守。SUMO1基因在猪心、肝、脾、肺、肾、小肠及肌肉组织中均有表达，且在肺部表达较高。猪肺炎支原体感染原代猪肺泡巨噬细胞后SUMO1的mRNA表达水平显著升高。参与调控炎症反应的基因，如TLR2、P65和RXRα均存在潜在的SUMO化修饰位点，并且猪肺炎支原体感染后它们的mRNA表达水平极显著升高。
Abstract:
The full-length upstream and downstream primers of SUMO1 (the gene of small ubiquitin-like modifier 1) were designed according to the base sequence of SUMO1 gene (NM_001112676.1) of GenBank on National Center for Biotechnology Information(NCBI) and the whole CDS(coding sequence) of porcine SUMO1 National Center for Biotechnology Information gene was cloned successfully. The results of bioinformatics software analysis showed that porcine SUMO1 gene-related protein CDS was 306 bp in length, encoded 101 amino acids and the amino acids sequence of SUMO1 protein was highly conserved among species. SUMO1 gene was expressed in porcine heart, liver, spleen, lung, kidney, small intestine and muscle, among which the relative expression of SUMO1 was higher in lung than in other tissues. After Mycoplasma hyopneumoniae infection, the mRNA expression level of SUMO1 was significantly increased in primary porcine alveolar macrophages. The potential SUMO modification sites were found in TLR2, P65 and RXRα genes, which participated in the regulation of inflammatory response. In addition, their mRNA expression levels were significantly up-regulated after Mycoplasma hyopneumoniae infection.

参考文献/References:

[1]SIMIONATTO S, MARCHIORO S B, MAES D, et al. Mycoplasma hyopneumoniae: from disease to vaccine development [J]. Vet Microbiol, 2013, 165(3/4): 234-42.
[2]邵国青，华利忠．混合感染背景下猪呼吸道病的免疫与控制[J]．中国兽药杂志，2012(46):97-99．
[3]MAES D, SIBILA M, KUHNERT P, et al. Update on Mycoplasma hyopneumoniae infections in pigs: Knowledge gags for improved disease control [J]. Transbound Emerg Dis, 2018, 65(Sup 1):110-124.
[4]OKAMURA T, ONODERA W, TAYAMA T, et al. A genome-wide scan for quantitative trait loci affecting respiratory disease and immune capacity in landrace pigs [J]. Animal Genetics, 2012, 43: 721-729.
[5]ENSERINK J M. Sumo and the cellular stress response [J]. Cell Div, 2015, 10:4.
[6]KLAUS S, PUCK K, EVERTVAN D, et al. SUMO Assay with peptide arrays on solid support: Insights into SUMO target sites [J]. The Journal of Biochemistry, 2008, 144(1):39-49.
[7]WANG C, ZENG N, LIU S, et al. Interaction of porcine reproductive and respiratory syndrome virus proteins with SUMO-conjugating enzyme reveals the SUMOylation of nucleocapsid protein [J]. PLoS One, 2017, 12(12):e0189191.
[8]YANG Y, HE Y, WANG X, et al. Protein SUMO ylation modification and its associations with disease [J]. Open Biol, 2017, 7(10):170167.
[9]ZHOU X, LI X, WANG X, et al. Cecropin B represses CYP3A29 expression through activation of the TLR2/4-NF-κB/PXR signaling pathway [J]. Science Reports, 2016,14(6):27876.
[10]赵为民，涂枫，方晓敏，等．猪PKM2基因的序列分析与组织表达及亚细胞定位[J]．湖南农业大学学报，2019，45(1)：8-77.
[11]方晓敏，赵为民，付言峰，等．猪支原体肺炎发生的品种敏感差异及分子基础[J]．中国农业科学，2015，48(14)：2839-2847．
[12]郭芸芸，张雪寒，刘茂军，等.猪肺炎支原体纤毛黏附因子P97和F7-CTB对O型口蹄疫灭活疫苗的免疫增强作用[J]. 江苏农业科学，2019,47(11):204-210．
[13]赵为民,涂枫,王丽,等. 猪PKM2基因在肺炎支原体感染3D4/21细胞后的互作蛋白质鉴定与分析[J].江苏农业学报, 2019,35(6):1381-1389.
[14]李彦伟，刘茂军，武昱孜，等．猪肺炎支原体致病机理研究进展[J]．动物医学进展，2013，34(8)：84-88．
[15]GOULOPOULOU S, MCCARTHY C G, WEBB R C. Toll-like receptors in the vascular system: sensing the dangers within [J]. Pharmacological Reviews, 2016, 68: 142-167.
[16]PAGE K, LIERL K M, HUGHES V S, et al. TLR2-mediated activation of neutrophils in response to German cockroach frass [J]. Journal of Immunology, 2008, 180: 6317-6324.
[17]LI X, CHEN Q, CHU C, et al. Ovalbumin-induced experimental allergic asthma is Toll-like receptor 2 dependent [J]. Allergy and Asthma Proceedings, 2014, 35: 15-20.
[18]KE C, ZHU K, SUN Y, et al. SUMO1 promotes the proliferation and invasion of non-small cell lung cancer cells by regulating NF-κB[J]. Thorac Cancer, 2019, 10(1):33-40.
[19]GAO X, WU Y, QIAO L, et al. SENP2 suppresses NF-κB activation and sensitizes breast cancer cells to doxorubicin[J]. Eur J Pharmacol, 2019, 854(5):179-186.
[20]REBECCA S, SAUL J. Inflammatory mediators increase SUMOylation of retinoid X receptor α in a c-Jun N- terminal kinase -dependent manner in human hepatocellular carcinoma cells [J]. Molecular Pharmacology, 2013, 84: 218-226.

备注/Memo

备注/Memo:
收稿日期：2019-12-30基金项目：国家自然科学基金项目（31872338、31601928）；国家生猪现代产业技术体系南京综合试验站项目(CARS-35)；高邮市科技计划项目（GY201914）作者简介：王丽(1988-)，女，湖北武汉人，博士，助理研究员，主要从事动物遗传育种与繁殖研究。(E-mail)wanglids@163.com。杨晓阳为共同第一作者。通讯作者：方晓敏，(E-mail)fxmw2000@163.com
更新日期/Last Update: 2020-11-16