[1]蔡张杰,崔丽娟,李晶,等.低温条件下人工湿地氨氧化微生物的群落结构特征[J].江苏农业学报,2020,(02):373-383.[doi:doi:10.3969/j.issn.1000-4440.2020.02.017]
　CAI Zhang-jie,CUI Li-juan,LI Jing,et al.Community structure characteristics of ammonia-oxidizing microorganisms in constructed wetland at low temperature[J].,2020,(02):373-383.[doi:doi:10.3969/j.issn.1000-4440.2020.02.017]
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低温条件下人工湿地氨氧化微生物的群落结构特征()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2020年02期
页码:
373-383
栏目:
耕作栽培·资源环境
出版日期:
2020-04-30

文章信息/Info

Title:
Community structure characteristics of ammonia-oxidizing microorganisms in constructed wetland at low temperature
作者:
蔡张杰1; 2; 3; 崔丽娟1; 2; 3; 李晶1; 2; 3; 李伟1; 2; 3; 雷茵茹1; 2; 3
（1.中国林业科学研究院湿地研究所，北京100091；2.湿地生态功能与恢复北京市重点实验室，北京100091；3.北京汉石桥湿地生态系统国家定位观测研究站，北京101399）
Author(s):
CAI Zhang-jie1; 2; 3; CUI Li-juan1; 2; 3; LI Jing1; 2; 3; LI Wei1; 2; 3; LEI Yin-ru1; 2; 3
(1.Institute of Wetland Research, Chinese Academy of Forest, Beijing 100091, China;2.The Beijing Key Laboratory of Wetland Ecological Function and Restoration, Beijing 100091, China;3.Beijing Hanshiqiao National Wetland Ecosystem Research Station, Beijing 101399, China)
关键词:
人工湿地; 硝化潜势; 功能基因丰度; 群落结构; 环境因子
Keywords:
constructed wetland; potential nitrification rate; functional genes′ abundance; community structure; environmental factor
分类号:
X171.1
DOI:
doi:10.3969/j.issn.1000-4440.2020.02.017
文献标志码:
A
摘要:
为了解冬季低温条件下人工湿地沉积物的氨氧化潜力及其影响因素，以北京市汉石桥湿地自然保护区内的人工湿地为研究对象，测定了不同湿地单元沉积物的硝化潜势（PNR）及其相关功能基因的丰度。结果表明，沉积物的PNR较高，说明冬季人工湿地具有较高的硝化潜力。PNR从入水段到出水段呈现先增加后减少的趋势。通过建立硝化活性与功能微生物丰度的线性回归模型发现，氨氧化古菌（AOA，以AOA的 amoA基因丰度表征）是影响硝化潜势的主要功能微生物。进一步构建硝化微生物丰度与环境因子的回归方程，发现影响AOA amoA基因丰度的主要环境因子是碳氮比（C/N），影响氨氧化细菌（AOB，以AOB的 amoA基因丰度表征）丰度的主要环境因子是全氮（TN）含量。高通量测序测定人工湿地沉积物AOA和AOB的群落组成，各湿地单元检测到的AOB群落优势门为Proteobacteria，主要是β-Proteobacteria纲，AOA主要属于Crenarchaeota和Thaumarchaeota 2个门类。湿地4单元和5单元，1单元和6单元在AOB菌群群落组成上较为接近；湿地6单元、7单元、8单元在AOA菌群群落组成上较为接近。
Abstract:
To understand the potential nitrification rate of the constructed wetland sediments under low temperature conditions in winter and its influencing factors, the constructed wetland in the Hanshiqiao Wetland Nature Reserve in Beijing was selected as the research area, and the potential nitrification rate (PNR) and the abundance of their related functional genes were investigated. The results showed that the PNR of the sediment was higher, indicating that the constructed wetland had higher nitrification potential in winter. The PNR increased first and then decreased from the inlet section to the outlet section. By establishing a linear regression model of nitrification activity and functional microbial abundance, we found that ammonia-oxidizing archaea (AOA, characterized by the abundance of amoA gene in AOA) was the main functional microorganism affecting the nitrification potential. By further constructing the regression equation of microbial abundance and environmental factors, it was found that the main environmental factor affecting the abundance of AOA amoA gene was carbon-nitrogen ratio (C/N), and the main environmental factor affecting the abundance of ammonia-oxidizing bacteria (AOB, characterized by abundance of amoA gene in AOB) was total nitrogen (TN) content. High-throughput sequencing was used to determine the community composition of AOA and AOB in constructed wetland sediments. The dominant phylum of AOB community in each wetland unit was Proteobacteria, which was mainly composed of β-subdivision of Proteobacteria, and AOA mainly belonged to Crenarchaeota and Thaumarchaeota. In terms of community composition, for AOB, wetland units 4 and 5, 1 and 6 were relatively similar in flora composition, for AOA, wetland units 6, 7, 8 were relatively close.

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备注/Memo

备注/Memo:
收稿日期：2019-06-03基金项目：国家重点研发计划项目（2017YFC0506200）；国家自然科学基金项目（41601577）作者简介：蔡张杰（1992-），男，河南郸城人，硕士研究生，主要从事湿地微生物生态学研究。（E-mail）caizhangjie2017@163.com通讯作者：崔丽娟,（E-mail）lkyclj@126.com
更新日期/Last Update: 2020-05-18