[1]刘强,柳正葳,刘召亮,等.一氧化氮对铝胁迫下烟草根系营养元素吸收和呼吸电子传递及内源激素含量的影响[J].江苏农业学报,2020,(02):292-298.[doi:doi:10.3969/j.issn.1000-4440.2020.02.006]
　LIU Qiang,LIU Zheng-wei,LIU Zhao-liang,et al.Effects of nitric oxide on nutrient uptake, respiratory electron transfer and endogenous phytohormones contents in roots of tobacco seedlings under aluminum stress[J].,2020,(02):292-298.[doi:doi:10.3969/j.issn.1000-4440.2020.02.006]
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一氧化氮对铝胁迫下烟草根系营养元素吸收和呼吸电子传递及内源激素含量的影响()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2020年02期
页码:
292-298
栏目:
遗传育种·生理生化
出版日期:
2020-04-30

文章信息/Info

Title:
Effects of nitric oxide on nutrient uptake, respiratory electron transfer and endogenous phytohormones contents in roots of tobacco seedlings under aluminum stress
作者:
刘强1; 柳正葳2; 刘召亮1; 乔清华1; 张玉1
（1.玉林师范学院生物与制药学院，广西玉林537000；2.井冈山大学生命科学学院，江西吉安343009）
Author(s):
LIU Qiang1; LIU Zheng-wei2; LIU Zhao-liang1; QIAO Qing-hua1; ZHANG Yu1
（1.College of Biology and Pharmacy， Yulin Normal University， Yulin 537000， China；2.College of Life Sciences， Jinggangshan University， Ji′an 343009， China）
关键词:
烟草; 铝胁迫; 一氧化氮; 营养元素; 呼吸作用; 植物激素
Keywords:
tobacco; aluminum stress; nitric oxide; nutrient elements; respiration; phytohormones
分类号:
Q945.78
DOI:
doi:10.3969/j.issn.1000-4440.2020.02.006
文献标志码:
A
摘要:
为探明一氧化氮供体硝普钠（SNP）对铝胁迫下烟草幼苗生长和根系生理特性的促进作用，以耐铝型品种云烟100和铝敏感型品种云烟105为材料，采用水培法研究施加不同浓度SNP（0 μmol/L、50 μmol/L、100 μmol/L、200 μmol/L、400 μmol/L）对铝胁迫（200 μmol/L）下烟草幼苗生长、根系营养元素吸收、呼吸电子传递及内源激素含量的影响。结果表明，铝胁迫下烟草生长受到明显抑制，根系营养元素（钙、镁、铁、铜）含量、各呼吸途径的呼吸速率（总呼吸、细胞色素呼吸、交替呼吸）和根尖生长素、赤霉素含量均显著下降，而根尖活性氧（O2·-、H2O2）和脱落酸含量显著升高。加入适宜浓度的SNP能显著缓解铝对烟草根系和地上部生长的抑制，提高根系钙、镁、铁、铜营养元素含量和各呼吸途径的呼吸速率，促进根尖生长素、赤霉素和脱落酸合成，并降低根尖活性氧（O2·-、H2O2）含量。在试验浓度范围内以施加100 μmol/L的SNP对烟草铝毒害缓解效果最佳，而SNP为400 μmol/L时缓解效果受到抑制。综上所述，100 μmol/L的SNP可通过促进烟草根系对钙、镁、铁和铜营养元素的吸收，提高根系各呼吸途径的呼吸速率以降低根尖活性氧含量，以及维持根尖内源激素的平衡而缓解烟草铝毒害。
Abstract:
Two tobacco cultivars of Yunyan 100 (aluminum-resistant) and Yunyan 105 (aluminum-sensitive) were selected to explore the effects of sodium nitroprusside (SNP, as the nitric oxide donor) with different concentrations (0 μmol/L, 50 μmol/L, 100 μmol/L, 200 μmol/L, 400 μmol/L) on plant growth, root nutrient elements uptake, respiratory electron transfer and endogenous hormones contents under aluminum stress (200 μmol/L) with solution culture. The results showed that aluminum stress significantly inhibited plant growth, decreased root nutrient elements (Ca, Mg, Fe, Cu) uptake, respiratory rate (total respiration, cytochrome respiration, alternative respiration), root apical auxin and gibberellin contents, but significantly increased the contents of reactive oxygen (O2·- and H2O2) and abscisic acid in root apex of tobacco seedlings. Application of SNP concentration dramatically relieved the aluminum-induced inhibition of root and aboveground growth, increased root nutrient elements (Ca, Mg, Fe, Cu) uptake, respiratory rate, the contents of auxin, gibberellin and abscisic acid in root apex, but dramatically decreased reactive oxygen (O2·- and H2O2) contents. In this experiment, SNP at 100 μmol/L had the best effects on relieving aluminum toxicity in tobacco seedlings, while the alleviating effects were suppressed when SNP concentration was 400 μmol/L. In conclusion, treatment with SNP at 100 μmol/L could alleviate aluminum toxicity in tobacco seedlings through promoting root nutrient elements (Ca, Mg, Fe, Cu) uptake, increasing respiratory rate in order to decrease reactive oxygen (O2·- and H2O2) accumulation, maintaining balance of endogenous phytohormones in root apex.

参考文献/References:

[1]刘强，郑绍建，林咸永.植物适应铝毒胁迫的生理及分子生物学机理 [J].应用生态学报，2004，15(9):1641-1649.
[2]陈文荣，刘鹏，黄朝表，等.铝对荞麦铝和其它营养元素运输的影响 [J].水土保持学报，2006，20(3):173-176.
[3]YAMAMOTO Y，KOBAYASHI Y，DEVI S R，et al.Aluminum toxicity is associated with mitochondrial dysfunction and the production of reactive oxygen species in plant cells [J].Plant Physiology，2002，128(1):63-72.
[4]PEREIRA L B，MAZZANTI C M，CARGNELUTTI D，et al. Differential responses of oat genotypes: oxidative stress provoked by aluminum [J].Biometals，2011，24(1):73-83.
[5]HE H Y，HE L F，GU M H，et al. Nitric oxide improves aluminum tolerance by regulating hormonal equilibrium in the root apices of rye and wheat [J].Plant Science，2012，183(2):123-130.
[6]YU M，LAMATTINA L，SPOEL S H，et al. Nitric oxide function in plant biology: a redox cue in deconvolution [J].New Phytologist，2014，202(4):1142-1156.
[7]SUN C L，LIU L J，LU L L，et al. Nitric oxide acts downstream of hydrogen peroxide in regulating aluminum-induced antioxidant defense that enhances aluminum resistance in wheat seedlings [J]. Environmental and Experimental Botany，2018，145(1):95-103.
[8]WANG H H，HUANG J J，LIANG W H，et al. Involvement of putrescine and nitric oxide in aluminum tolerance by modulating citrate secretion from roots of red kidney bean [J].Plant and Soil，2013，366(2):479-490.
[9]ZHANG Z Y，WANG H H，WANG X M，et al. Nitric oxide enhances aluminum tolerance by affecting cell wall polysaccharides in rice roots [J].Plant Cell Reports，2011，30(9):1701-1711.
[10]YANG L M，TIAN D G，TODD C D，et al. Comparative proteome analyses reveal that nitric oxide is an important signal molecule in the response of rice to aluminum toxicity [J].Journal of Proteome Research，2013，12(3):1316-1330.
[11]ZHOU Y，XU Y Y，CHEN L Q，et al. Nitric oxide exacerbates Al-induced inhibition of root elongation in rice bean by affecting cell wall and plasma membrane properties [J].Phytochemistry，2012，76(1):46-51.
[12]WANG H H，HUANG J J，BI Y R. Nitrate reductase-dependent nitric oxide production is involved in aluminum tolerance in red kidney bean roots [J]. Plant Science，2010，179(3):281-288.
[13]尹永强，何明雄，邓明军.土壤酸化对土壤养分及烟叶品质的影响及改良措施 [J]. 中国烟草科学，2008，29(1):51-54.
[14]刘强，贺根和，柳正葳，等.外源一氧化氮对铝胁迫下烟草叶片光能利用和光保护系统及活性氧代谢的影响[J]. 湖南农业大学学报，2016，42(6):615-621.
[15]刘强，柳正葳，胡萃，等.外源一氧化氮对铝胁迫下烟草叶片呼吸作用和活性氧代谢的影响[J]. 河南农业科学，2017，46(1):42-47.
[16]HU W H，XIAO Y A，ZENG J J，et al. Photosynthesis, respiration and antioxidant enzymes in pepper leaves under drought and heat stresses [J]. Biologia Plantarum，2010，54(4):761-765.
[17]ZHOU Y H，YU J Q，HUANG L F，et al. The relationship between CO2 assimilation, photosynthetic electron transport and water-water cycle in chill-exposed cucumber leaves under low light and subsequent recovery [J]. Plant Cell and Environment，2004，27(12):1503-1514.
[18]王志颖，刘鹏，李锦山，等.铝胁迫下外源有机酸对油菜根系形态及叶绿素荧光特性的影响[J]. 江苏农业学报，2011，27(4):756-762.
[19]于力，孙锦，郭世荣，等.铝胁迫对豇豆幼苗根系生理指标的影响[J]. 江苏农业学报，2013，29(2):319-323.
[20]CAI M Z，ZHANG S N，WANG F M，et al. Protective effect of exogenously applied nitric oxide on aluminum induced oxidative stress in soybean plants [J]. Russian Journal of Plant Physiology，2011，58(5):791-798.
[21]SUN C L，LIU L J，YU Y，et al. Nitric oxide alleviates aluminum-induced oxidative damage through regulating the ascorbate-glutathione cycle in roots of wheat [J]. Journal of Integrative Plant Biology，2015，57(6):550-561.
[22]CORPAS F J，LETERRIER M，VALDERRAMA R，et al. Nitric oxide imbalance provokes a nitrosative response in plants under abiotic stress [J]. Plant Science，2011，181(5):604-611.
[23]RENGEL Z，ROBINSON D L. Aluminum and plant age effects on absorption of cations in the Donnan free space of rye grass roots [J]. Plant Soil，1989，116(2):223-227.
[24]潘瑞炽.植物生理学 [M]. 北京:高等教育出版社，2012.
[25]段小华，邓泽元，胡小飞，等.模拟酸雨和外源铝对茶树铝及一些营养元素吸收积累的影响[J]. 农业环境科学学报，2010，29(10):1936-1942.
[26]HUANG J W，PELLET D M，PAPERNIK L A，et al. Aluminum interactions with woltage dependant calcium transport in plasma membrane vesicle isolated from roots of aluminum-sensitive and resistant wheat cultivars [J]. Plant Physiology，1996，110(2):561-569.
[27]袁世力，刘星辰，樊娜娜，等.镁对铝胁迫紫花苜蓿幼苗生长和光合系统的影响[J]. 中国草地学报，2018，40(5):29-35.
[28]DAVIDSON J F，SCHIESTL R H. Mitochondrial respiratory electron carriers are involved in oxidative stress during heat stress in Saccharomyces cerevisiae [J]. Molecular and Cellular Biology，2001，21(24):8483-8489.
[29]谢国生，师瑞红，庞贞武，等.铝胁迫下水稻幼苗根系的生理特性[J]. 应用生态学报，2009，20(7):1698-1704.
[30]KOPITTKE P M. Role of phytohormones in aluminium rhizotoxicity [J]. Plant Cell and Environment，2016，39(10):2319-2328.
[31]赵爽，郑刚，季梦成，等. 山木通扦插生根过程中内源激素的动态变化[J]．江苏农业科学，2018，46(3)：148-150.
[32]任晓燕，周鹏，安渊.喷施IAA对紫花苜蓿幼苗铝毒害的缓解作用[J]. 草业科学，2014，31(7):1323-1329.
[33]李婧，刘星星，吴玉环，等.外源脱落酸对铝胁迫下栝楼叶绿素荧光及生理活性的影响[J]. 水土保持学报，2017，31(2):293-300.
[34]SHEN H，LIGABA A，YAMAGUCHI M，et al. Effect of K-252a and abscisic acid on the efflux of citrate from soybean roots [J]. Journal of Experimental Botany，2004，55(397):663-671.
[35]KASAI M，SASAKI M，TANAKAMARU S，et al. Possible involvement of abscisic acid in increases in activities of two vacuolar H+-pumps in barley roots under aluminum stress[J]. Plant Cell Physiology，1993，34(8):1335-1338.

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备注/Memo

备注/Memo:
收稿日期：2019-06-12基金项目：国家自然科学基金项目(31960632、31260498)作者简介：刘强(1980-)，男，江西抚州人，博士，教授，研究方向为植物营养逆境生理与分子生物学。(E-mail)qliu2006@163.com
更新日期/Last Update: 2020-05-18