[1]张宽朝,陈杰,马伟,等.响应面法优化草莓总黄酮提取工艺[J].江苏农业学报,2019,(06):1450-1458.[doi:doi:10.3969/j.issn.1000-4440.2019.06.026]
　ZHANG Kuan-chao,CHEN Jie,MA Wei,et al.Optimization on extraction technology of total flavonoids from strawberry by response surface methodology[J].,2019,(06):1450-1458.[doi:doi:10.3969/j.issn.1000-4440.2019.06.026]
点击复制

响应面法优化草莓总黄酮提取工艺()
分享到：

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2019年06期
页码:
1450-1458
栏目:
加工贮藏·质量安全
出版日期:
2019-12-31

文章信息/Info

Title:
Optimization on extraction technology of total flavonoids from strawberry by response surface methodology
作者:
张宽朝1; 陈杰2; 马伟3; 何孔泉1; 阮飞1
（1.安徽农业大学生命科学学院，安徽合肥230036；2.淮南市科技情报研究所，安徽淮南232008；3.寿县瓦埠湖农业种植专业合作社，安徽淮南232212）
Author(s):
ZHANG Kuan-chao1; CHEN Jie2; MA Wei3; HE Kong-quan1; RUAN Fei1
(1. School of Life Science, Anhui Agriculture University, Hefei 230036, China; 2. Huainan Institute of Science and Technology Information, Huainan 232008, China; 3. Shouxian Wabuhu Agricultural Planting Specialized Cooperative, Huainan 232212, China)
关键词:
草莓; 总黄酮; 提取; 响应面优化
Keywords:
strawberry; total flavonoids; extraction; response surface optimization
分类号:
TS202.3
DOI:
doi:10.3969/j.issn.1000-4440.2019.06.026
文献标志码:
A
摘要:
以草莓为试验材料，在单因素试验的基础上，采用Box-Behnken设计，利用响应面分析法探讨乙醇体积分数、提取温度、液料比、提取时间4个因素对草莓总黄酮提取得率的影响。结果表明，各因素对草莓总黄酮提取得率影响大小顺序为液料比＞乙醇体积分数＞提取时间＞提取温度；建立了草莓总黄酮提取工艺参数的二次多项式回归模型，由该模型优化、修正的草莓总黄酮提取条件为：乙醇体积分数60%、提取温度53 ℃、液料比47∶1(体积质量比)、提取时间120 min，在此条件下，草莓总黄酮得率达到5.89 mg/g，与模型预测结果相近。本研究结果为草莓总黄酮类物质工业化生产的后续研究提供了参考。
Abstract:
Based on the single factor experiment and Box-Behnken design, the effects of ethanol volume fraction, extraction temperature, liquid-to-solid ratio and extraction time on the extraction of total flavonoids from strawberry were studied by response surface methodology. The results showed that the order of factors affecting the extraction yield of total flavonoids from strawberry was liquid-to-solid ratio>ethanol volume fraction>extraction time>extraction temperature. The quadratic polynomial regression model of extraction parameters for total flavonoids from strawberry was established. The extraction conditions of total flavonoids from strawberry optimized and modified by the model were as follows: ethanol volume fraction 60%, extraction temperature 53 ℃, liquid-to-solid ratio 47∶1, extraction time 120 min. Under these conditions, the yield of total flavonoids from strawberry was 5.89 mg/g, which was similar to the prediction result of the model. The results of this study provide a reference for the follow-up study on industrialized production of total flavonoids in strawberry.

参考文献/References:

[1]KALT W, FORNEY C F, MARTIN A, et al. Antioxidant capacity vitamin C,phenolics, and anthocyanins after fresh storage of small fruits[J]. Journal of Agricultural and Food Chemistry, 1999, 47(11): 4638-4644
[2]苏代发，童江云，杨俊誉，等.中国草莓属植物种质资源的研究、开发与利用进展[J].云南大学学报( 自然科学版) ，2018，40(6):1261-1276
[3]余璐璐，曹中权，朱春娇，等.不同浓度水杨酸处理对草莓采后保鲜的影响[J].植物生理学报,2015, 51 (11): 2047-2053.
[4]吉沐祥，杨勇，彭燕琼，等．江苏草莓生产现状与消费需求调查分析及其发展建议[J]．江苏农业科学，2017，45(16)：336-340.
[5]罗学兵，贺良明.草莓的营养价值与保健功能[J].中国食物与营养, 2011, 17(4): 74-76.
[6]扶庆权，徐鉴.正交试验法优化草莓中总黄酮的提取工艺研究[J].中国食品添加剂，2011（6）：130-135.
[7]延玺，刘会青，邹永青，等.黄酮类化合物生理活性及合成研究进展[J].有机化学，2008,28（9）：1534-1544.
[8]扶庆权，侯佩，陈能.响应面法优化芦蒿叶总黄酮的提取工艺[J].食品科学，2013，34（4）：94-98.
[9]LIU Y H,MOU X,ZHOU D Y,et al.Extraction of flavonoids from Chrysanthemum morifolium and antitumor activity in vitro[J]. Experimental & Therapeutic Medicine,2018,15(2):1203-1210.
[10]SARAH M, MOHAMED K. Enhancement of total phenolic and flavonoids extraction from Rosmarinus officinalis L. using electromagnetic induction heating (EMIH) process[J]. Physiology and Molecular Biology of Plants,2018,24（5）：889-897.
[11]LIU Y,LUO Y, LAN Z Q,et al.Ultrasonic-assisted extraction and antioxidant capacities of flavonoids from Camellia fascicularis leaves[J]. CyTA - Journal of Food,2018,16(1):105-112.
[12]LIANG G Y,DENG Y,JU T, et al.Continuous high voltage electrical discharge extraction of flavonoids from peanut shells based on ‘annular gap type’ treatment chamber[J].Food Chemistry,2018,256(1):350-357.
[13]袁媛，余修亮，陈宇欢，等.桃金娘花青素和黄酮的提取方法比较及其抗氧化能力研究[J].中国食品学报，2018，18（9）：144-151.
[14]PARK D,PARK Y, LEE Y H, et al.A Comparative study of phenolic antioxidant activity and flavonoid biosynthesis-related gene expression between summer and winter strawberry cultivars[J]. J Food Sci,2017，82(2):341-349.
[15]CRECELIUS A C, HLSCHER D,HOFFMANN T, et al.Spatial and temporal localization of flavonoid metabolites in strawberry fruit (Fragaria × Ananassa) [J]. J Agric Food Chem, 2017, 65 (17): 3559-3568.
[16]冯晨静,关军锋,杨建民,等.草莓果实成熟期花青苷、酚类物质和类黄酮含量的变化[J].果树学报，2003,20（3）：199-201.
[17]严嘉玮,路洪艳,李莉，等.2 种草莓成熟过程中果实特征品质指标的变化[J].食品科学，2017,38（4）：125-132.
[18]李志洲,刘军海. 草莓中黄酮的提取及其抗氧化性研究[J].食品研究与开发，2007,128（7）：31-34.
[19]张宽朝，文汉，胡雅萍，等.贡菊黄酮抗小鼠急性肝损伤作用的研究[J].天然产物研究与开发，2014，26（2）:183-187，214.
[20]邵金华，李涛，杨艳.微波辅助工业乙醇提取废弃洋葱皮中总黄酮工艺研究[J].江苏农业科学，2013，41(3):237-239.
[21]LIU W,YU Y Y,YANG R Z.Optimization of total flavonoid compound extraction from gynura medica leaf using response surface methodology and chemical composition analysis[J]. International Journal of Molecular Sciences,2010,11(11):4750-4763.
[22]孙朦,王鸿飞,李艳霞,等.辣木叶总黄酮提取工艺优化及抗氧化能力研究[J].核农学报，2018，32(9):1772-1780.
[23]崔美林，苏玉芳，高红.不同加工方式对基于双水相体系提取的小米黄酮的影响[J].食品与发酵工业，2018，44(5):156-161.
[24]焦昆鹏,马丽苹,朱文学,等.响应面法优化花生茎叶黄酮的提取工艺及其抗氧化活性研究[J].中国粮油学报，2018,33（2）：89-95.
[25]朱海霞,张小芳,高洋，等.萝卜籽粕中黄酮的提取及纯化工艺研究[J].食品与机械，2018,34（1）：159-162.
[26]熊苏慧，唐洁，李诗卉，等.一种新型天然低共熔溶剂用于玉竹总黄酮的绿色提取[J].中草药，2018,49（10）：2378-2386.
[27]范金波，蔡茜彤，冯叙桥，等.牛蒡根多酚和黄酮超高压提取工艺优化及体外抗氧化活性[J].食品科学，2015,36（6）：69-75.
[28]张豫超,谢鸣,陈俊伟,等.不同采收期草莓果实抗氧化物质含量和抗氧化活性的变化[J]. 浙江农业学报，2009，21( 3):250-254.
[29]刘文旭，黄午阳，曾晓雄，等. 草莓、黑莓、蓝莓中多酚类物质及其抗氧化活性研究[J].食品科学,2011,32(23):130-133.

相似文献/References:

[1]巫建华,贾思振,冯英娜,等.茉莉酸合成关键酶基因FaOPR3调控草莓果实成熟[J].江苏农业学报,2016,(05):1148.[doi:10.3969/j.issn.1000-4440.2016.05.031]
　WU Jian-hua,JIA Si-zhen,FENG Ying-na,et al.Regulation of strawberry fruit ripening by jasmonic acid synthesis gene FaOPR3[J].,2016,(06):1148.[doi:10.3969/j.issn.1000-4440.2016.05.031]
[2]赵密珍,庞夫花,袁华招,等.不同栽培条件下草莓品种宁玉花序分化进程[J].江苏农业学报,2016,(01):196.[doi:10.3969/j.issn.1000-4440.2016.01.030 ]
　ZHAO Mi-zhen,PANG Fu-hua,YUAN Hua-zhao,et al.Floral bud differentiation of strawberry Ningyu under conditions of forcing cultivation and open field cultivation[J].,2016,(06):196.[doi:10.3969/j.issn.1000-4440.2016.01.030 ]
[3]王兴娜,汪晶,黄午阳,等.青梅果实不同极性组分的抗氧化活性[J].江苏农业学报,2016,(01):211.[doi:10.3969/j.issn.1000-4440.2016.01.033 ]
　WANG Xing-na,WANG Jing,HUANG Wu-yang,et al.Antioxidative activities of different polar extracts from Prunus mume fruits[J].,2016,(06):211.[doi:10.3969/j.issn.1000-4440.2016.01.033 ]
[4]吴祥,姚克兵,吉沐祥,等.句容地区草莓枯萎病病原菌的分离鉴定及田间防治[J].江苏农业学报,2015,(04):764.[doi:10.3969/j.issn.1000-4440.2015.04.009]
　WU Xiang,YAO Ke-bing,JI Mu-xiang,et al.Isolation and identification and field control of strawberry fusarium wilt in Jurong, Jiangsu province[J].,2015,(06):764.[doi:10.3969/j.issn.1000-4440.2015.04.009]
[5]周游,李海梅,赵金山,等.乳酸菌对草莓生长和品质性状的影响[J].江苏农业学报,2017,(05):1124.[doi:doi:10.3969/j.issn.1000-4440.2017.05.025]
　ZHOU You,LI Hai-mei,ZHAO Jin-shan,et al.Effects of lactic acid bacteria on the growth and quality characters of strawberry[J].,2017,(06):1124.[doi:doi:10.3969/j.issn.1000-4440.2017.05.025]
[6]江景勇,王会福,何玲玲,等.台州草莓农药残留风险评估[J].江苏农业学报,2017,(06):1408.[doi:doi:10.3969/j.issn.1000-4440.2017.06.030]
　JIANG Jing-yong,WANG Hui-fu,HE Ling-ling,et al.Risk assessment of pesticide residues in strawberry from Taizhou city[J].,2017,(06):1408.[doi:doi:10.3969/j.issn.1000-4440.2017.06.030]
[7]汪燕,廖凯,喻武鹃,等.苦荞耐低磷力鉴定及其产量和品质分析[J].江苏农业学报,2018,(03):503.[doi:doi:10.3969/j.issn.1000-4440.2018.03.004]
　WANG Yan,LIAO Kai,YU Wu-juan,et al.Identification of low-phosphorus tolerance and analysis of yield and quality in tartary buckwheat[J].,2018,(06):503.[doi:doi:10.3969/j.issn.1000-4440.2018.03.004]
[8]丛嘉昕,宋江峰,李大婧,等.基于RSM和MLP-ANN的草莓果浆超声酶解参数优化[J].江苏农业学报,2018,(06):1354.[doi:doi:10.3969/j.issn.1000-4440.2018.06.022]
　CONG Jia-xin,SONG Jiang-feng,LI Da-jing,et al.Optimization of ultrasonic enzymolysis of strawberry pulp based on RSM and MLP-ANN[J].,2018,(06):1354.[doi:doi:10.3969/j.issn.1000-4440.2018.06.022]
[9]姜玉素,李珍,王庆莲,等.草莓KEA家族基因的克隆、鉴定及表达分析[J].江苏农业学报,2019,(02):391.[doi:doi:10.3969/j.issn.1000-4440.2019.02.021]
　JIANG Yu-su,LI Zhen,WANG Qing-lian,et al.Cloning, characterization and expression analysis of KEA family genes in strawberry[J].,2019,(06):391.[doi:doi:10.3969/j.issn.1000-4440.2019.02.021]
[10]王廷峰,赵密珍,关玲,等.玉米套作及秸秆还田对草莓连作土壤养分及微生物区系的影响[J].江苏农业学报,2019,(06):1421.[doi:doi:10.3969/j.issn.1000-4440.2019.06.022]
　WANG Ting-feng,ZHAO Mi-zhen,GUAN-Ling,et al.Effects of intercropping with corn and straw returning on nutrients and microflora in strawberry continuous cropping soil[J].,2019,(06):1421.[doi:doi:10.3969/j.issn.1000-4440.2019.06.022]

备注/Memo

备注/Memo:
收稿日期：2019-03-05 基金项目：安徽省淮南市科技创新专项（2018A351） 作者简介：张宽朝（1981-），男，安徽涡阳人，硕士，高级实验师，主要从事生物化学与化学生物学研究。（E-mail）8058186@qq.com
更新日期/Last Update: 2020-01-09