[1]陈瑛琪,蔡瑶,龚双燕,等.四川地区一株猪塞内加谷病毒的分离鉴定及VP1基因的序列分析[J].江苏农业学报,2019,(06):1390-1396.[doi:doi:10.3969/j.issn.1000-4440.2019.06.017]
　CHEN Ying-qi,CAI Yao,GONG Shuang-yan,et al.Isolation and identification of the Seneca Valley virus strains in Sichuan province and analysis of VP1 gene sequences[J].,2019,(06):1390-1396.[doi:doi:10.3969/j.issn.1000-4440.2019.06.017]
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四川地区一株猪塞内加谷病毒的分离鉴定及VP1基因的序列分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2019年06期
页码:
1390-1396
栏目:
畜牧兽医·水产养殖
出版日期:
2019-12-31

文章信息/Info

Title:
Isolation and identification of the Seneca Valley virus strains in Sichuan province and analysis of VP1 gene sequences
作者:
陈瑛琪1; 蔡瑶1; 龚双燕1; 李小璟1; 李雨濛1; 李幽幽1; 徐逸飞1; 徐志文1; 2; 朱玲1; 2
(1.四川农业大学动物医学院，四川成都611130;2.四川农业大学动物疫病与人类健康四川省重点实验室，四川成都611130)
Author(s):
CHEN Ying-qi1; CAI Yao1; GONG Shuang-yan1; LI Xiao-jing1; LI Yu-meng1; LI You-you1; XU Yi-fei1; XU Zhi-wen1; 2; ZHU Ling1; 2
(1.College of Veterinary Medicine， Sichuan Agricultural University， Chengdu 611130， China;2.Key Laboratory of Animal Disease and Human Health of Sichuan Province， Sichuan Agricultural University， Chengdu 611130， China)
关键词:
猪塞内加谷病毒; 分离; 鉴定; VP1基因; 序列分析
Keywords:
Seneca Valley virus; isolation; identification; VP1 gene; sequence analysis
分类号:
S852.65;Q781
DOI:
doi:10.3969/j.issn.1000-4440.2019.06.017
文献标志码:
A
摘要:
猪塞内加谷病毒(Seneca Valley virus，SVV)是一种新出现的小RNA病毒科病毒，感染该病毒的猪群，尤其是仔猪可能出现严重的病理症状甚至死亡。2015年之前，该病毒主要流行于美国、加拿大及巴西等地区，并于2015年3月传入中国[1]，给养猪业带来了严重的经济损失。2018年2月，本实验室检测并收集了阳性病料，采用PK-15细胞对病料进行病毒分离，并通过观察细胞CPE（细胞病变效应）、RT-PCR扩增和VP1基因序列测定进行鉴定。结果显示，成功分离到四川地区第一株SVV并将其命名为CH-MS-2018。对VP1基因的扩增测序及序列分析结果表明，本次分离得到的CH-MS-2018株VP1基因与GenBank上分别于2015年分离的2株和2016年分离的3株美国株的亲缘关系较近，同源性为98.6%。而与最早分离的SVV原始株关系较远，仅为88.0%～89.8%。本研究结果为进一步探索四川地区SVV的生物学性质奠定了基础。
Abstract:
Seneca Valley virus (SVV) is an emerging virus of the family Picornaviridae. Pigs infected with SVV, especially piglets, may have severe pathological symptoms or even death. Before 2015, the disease was mainly prevalent in the United States, Canada and Brazil, and was introduced into China in March 2015 [1]. The epidemic brought serious economic losses to the pig industry. In February 2018, the laboratory of animal biotechology center used the PCR technology to detect and collect a number of SVV-positive materials. The virus was isolated from samples by PK-15 cells, and identified by cytopathic effect(CPE), RT-PCR amplification and VP1 gene sequencing. The results showed that the forst SVV strain in Sichuan province was successfully isolated and named as CH-MS-2018. According to the available data in the GenBank database, the VP1 gene of CH-MS-2018 strain was closely related to that of two American strains isolated in 2015 and three American strains isolated in 2016, with the homology of 98.6%. However, the relationship between VP1 gene of CH-MS-2018 strain and its original strain was far away, with the homology of 88.0%-89.8%. The results of this study lay the foundation further exploring the biological properties of SVV in Sichuan province.

参考文献/References:

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[8]赵晓亚,伍绮文,伍子娴,等. 国内首株猪塞内加谷病毒(Seneca Valley virus)的分离鉴定[J].中国预防兽医学报，2016，38(11):839-843.
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备注/Memo

备注/Memo:
收稿日期：2019-01-10 基金项目：四川省“十三五”育种攻关项目（2016NYZ0052）；国家“十二五”科技支撑计划项目（2015BAD12B04-2.3国家农业产业技术体系四川兽药创新团队专项（CARS-SVDIP） 作者简介：陈瑛琪（1994-），女，四川成都人，硕士研究生，主要从事动物传染病病原分子生物学方向的研究。（E-mail）865659992@qq.com 通讯作者：徐志文，（Tel）13981604765；（E-mail）abtcxzl72@126.com
更新日期/Last Update: 2020-01-09