[1]李春宏,殷剑美,王立,等.连作对芋头根际土壤理化性状和微生物特性的影响[J].江苏农业学报,2019,(04):825-833.[doi:doi:10.3969/j.issn.1000-4440.2019.04.011]
　LI Chun hong,YIN Jian mei,WANG Li,et al.Effects of continuous cropping on soil physicochemical and microbial properties of taro[J].,2019,(04):825-833.[doi:doi:10.3969/j.issn.1000-4440.2019.04.011]
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连作对芋头根际土壤理化性状和微生物特性的影响()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2019年04期
页码:
825-833
栏目:
耕作栽培·资源环境
出版日期:
2019-08-31

文章信息/Info

Title:
Effects of continuous cropping on soil physicochemical and microbial properties of taro
作者:
李春宏; 殷剑美; 王立; 蒋璐; 郭文琦; 韩晓勇; 张培通
（江苏省农业科学院经济作物研究所，江苏南京210014）
Author(s):
LI Chunhong; YIN Jianmei; WANG Li; JIANG Lu; GUO Wenqi; HAN Xiaoyong; ZHANG Peitong
(Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014， China)
关键词:
芋头; 连作; 根际; 土壤理化性状; 土壤微生物
Keywords:
taro; continuous cropping; rhizosphere; soil physicochemical properties; soil microbe
分类号:
S6323
DOI:
doi:10.3969/j.issn.1000-4440.2019.04.011
文献标志码:
A
摘要:
为探索芋头连作对土壤理化性状和微生物性状的影响，对芋头头茬和连作2年的根际土壤理化性状进行测定，用荧光定量 PCR技术与Illumina 高通量测序检测细菌和真菌数量、群落结构。连作芋头根际土壤有机质、碱解氮、速效磷、钙、镁、铁含量较头茬下降显著，而速效钾、锌含量显著增加。连作细菌与真菌的基因拷贝数显著高于头茬。连作芋头根际土壤细菌群落多样性指数、相对丰度较头茬差异较小，但连作芋头根际土壤真菌群落多样性指数显著高于头茬，且门、属的丰度与头茬有较大的差异，其中连作镰刀菌属、亡革菌属、周刺座霉属、小画线壳属的丰度较头茬大幅增加。连作改变了根际土壤营养元素含量，造成根际土壤细菌与真菌数量显著高于头茬，引起了土壤致病真菌丰度大幅增加。
Abstract:
To explore effects of continuous cropping of taro on soil physicochemical and microbial properties, the physicochemical properties of rhizosphere soil were investigated on 2year continuouscropping and new cropping of taro, and the number and community structure of bacteria and fungi were revealed by realtime PCR and Illumina Miseq sequencing platform. The contents of organic matter, alkalihydrolyzable N, available P, Ca, Mg, Fe in the rhizosphere soils for continuouscropping were significantly lower than those for new cropping, while the contents of available K, Zn were significantly higher than those for new cropping. The number of gene copies of bacteria and fungi in rhizosphere soil of continuous cropping was significantly higher than that of new cropping. The bacterial community diversity index and relative abundance in the rhizosphere soils for continuouscropping were not significantly different from those for new cropping, but the diversity index of fungal community for continuouscropping was significantly higher than that for new cropping. Furthermore, the abundance of phylum and genus level for continuouscropping differed greatly from that for new cropping, the abundance of Fusarium, Thanatephorus, Volutella and Monographella for continuouscropping was significantly higher than that for new cropping. Continuouscropping seriously changed the balance of nutrient contents in rhizosphere soil, resulted in more number of bacteria and fungi, and led to more abundance of soil pathogenic fungi.

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备注/Memo

备注/Memo:
收稿日期：2018-11-28 基金项目：江苏省农业科技自主创新基金项目［CX(2017)3045］ 作者简介：李春宏（1972-），男，江苏射阳人，博士，副研究员，主要从事芋头栽培技术研究。(Tel)025-84390373;(E-mail)2350772535@qq.com 通讯作者：张培通，(E-mail)1196764929@qq.com
更新日期/Last Update: 2019-08-31