[1]蒋葛,沈辉,万夕和,等.凡纳滨对虾急性肝胰腺坏死综合症病虾与健康虾肠道优势菌群比较分析[J].江苏农业学报,2019,(01):142-148.[doi:doi:10.3969/j.issn.1000-4440.2019.01.021]
　JIANG Ge,SHEN Hui,WAN Xi-he,et al.Difference analysis of intestinal flora between healthy Litopenaeus vannamei and acute hepatopancreatic necrosis syndrome L. vannamei[J].,2019,(01):142-148.[doi:doi:10.3969/j.issn.1000-4440.2019.01.021]
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凡纳滨对虾急性肝胰腺坏死综合症病虾与健康虾肠道优势菌群比较分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2019年01期
页码:
142-148
栏目:
畜牧兽医·水产养殖
出版日期:
2019-02-26

文章信息/Info

Title:
Difference analysis of intestinal flora between healthy Litopenaeus vannamei and acute hepatopancreatic necrosis syndrome L. vannamei
作者:
蒋葛1; 2; 沈辉2; 万夕和1; 2; 乔毅2; 黎慧2; 王李宝2; 史文军2
(1.上海海洋大学国家水生动物病原库，上海201306；2.江苏省海洋水产研究所，江苏南通226007)
Author(s):
JIANG Ge1; 2; SHEN Hui2; WAN Xi-he1; 2; QIAO Yi2; LI Hui2; WANG Li-bao2; SHI Wen-jun2
（1.National Pathogen Collection Center for Aquatic Animals, Shanghai Ocean University, Shanghai 201306, China;2.China Jiangsu Institute of Marine Fisheries, Nantong 226007, China）
关键词:
对虾急性肝胰腺坏死综合症; 凡纳滨对虾; 肠道微生态
Keywords:
acute hepatopancreatic necrosis syndrome; Litopenaeus vannamei; intestinal flora
分类号:
S945.1
DOI:
doi:10.3969/j.issn.1000-4440.2019.01.021
文献标志码:
A
摘要:
为研究急性肝胰腺坏死综合征（Acute hepatopancreatic necrosis syndrome，AHPNS）病原对凡纳滨对虾肠道菌群的影响，获取更多的AHPNS防治基础资料，采用PCR-RFLP方法比较了江苏地区患AHPNS病虾和健康对虾肠道微生物群落。结果发现，不同生长阶段病虾肠道中均只有弧菌属细菌；对照的健康对虾中，幼虾肠道优势菌为假单胞菌属、食酸菌属和固氮螺菌属细菌；养殖中期对虾肠道优势菌为发光杆菌属、弧菌属和希瓦氏菌属细菌；养殖后期对虾肠道优势菌为弧菌属、红杆菌属、莱茵海默氏菌属细菌。研究结果表明，凡纳滨对虾患AHPNS后肠道微生物群落明显不同于健康对虾，肠道菌群多样性遭到破坏，弧菌为肠道内绝对优势细菌，不同生长阶段健康对虾的肠道优势菌存在差异。本研究探究了AHPNS病原对于凡纳滨对虾肠道菌群的影响，并为认识和防控AHPNS提供试验基础。
Abstract:
In order to study the effect of acute hepatopancreatic necrosis syndrome (AHPNS) pathogeny on the intestinal microflora of L. vannamei and acquire more basic data for prevention and control of AHPNS, showed the difference between the intestinal microflora of AHPNS positive shrimp and healthy shrimp was compared by PCR-RFLP. The results showed that there were only Vibrio bacteria in intestine of diseased shrimp at different growth stages. Using healthy shrimp as contorl, dominant intestinal bacteria of juvenile shrimp were Pseudomonas, Acidovoraxs and Brevundimonas, and dominant intestinal bacteria of breeding mid-term shrimpwere Vibrio, Photobacterium and Shewanella, dominant intestinal bacteria of breeding late shrimp were Vibrio, Rhodobacteraceae and Rheinheimera. The results showed that the intestinal microbial community of L. vannamei infected with AHPNS was significantly different from that of healthy L. vannamei, and the diversity of intestinal flora was destroyed. Vibrio was the dominant bacteria in the intestine, and there were differences in intestinal dominant bacteria of healthy shrimp at different growth stages. This study explores the effect of AHPNS on intestinal flora, and provides experimental basis for recognition and prevention and control AHPNS.

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备注/Memo

备注/Memo:
收稿日期：2018-06-11 基金项目：江苏省渔业科技项目（Y2017-20）；江苏省重点研发计划（现代农业）项目（BE2017384）；江苏省农业科技自主创新基金项目[CX(18）2010]；南通市应用基础研究-民生项目（MS12015076）；江苏省苏北科技发展计划项目（BN2015120）；中央财政农业技术推广项目（TG(16)099） 作者简介：蒋葛（1993-），男，江苏溧阳人，硕士研究生，主要从事水生动物病原微生物学研究。(Email)18751322127@163.com。沈辉为共同第一作者。 通讯作者：万夕和，（Email）wxh1708@163.com
更新日期/Last Update: 2019-02-27