[1]陈博雯,郑圣晗,张莘,等.不同鸡种BF1基因SNP和Indel比对分析[J].江苏农业学报,2018,(05):1087-1094.[doi:doi:10.3969/j.issn.1000-4440.2018.05.017]
 CHEN Bo-wen,ZHENG Sheng-han,ZHANG Xin,et al.Comparative analysis of SNP and Indel of BF1 gene in different chicken breeds[J].,2018,(05):1087-1094.[doi:doi:10.3969/j.issn.1000-4440.2018.05.017]
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不同鸡种BF1基因SNP和Indel比对分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2018年05期
页码:
1087-1094
栏目:
畜牧兽医·水产养殖
出版日期:
2018-10-25

文章信息/Info

Title:
Comparative analysis of SNP and Indel of BF1 gene in different chicken breeds
作者:
陈博雯郑圣晗张莘王统苗王志秀张扬陈国宏常国斌
(扬州大学动物科学与技术学院,江苏扬州225009)
Author(s):
CHEN Bo-wenZHENG Sheng-hanZHANG XinWANG Tong-miaoWANG Zhi-xiuZHANG YangCHEN Guo-hongCHANG Guo-bin
(College of Animal Science and Technology,Yangzhou University,Yangzhou 225009,China)
关键词:
鸡MHCBF1基因SNPINDEL
Keywords:
chicken MHCBF1 geneSNPIndel
分类号:
S858.31
DOI:
doi:10.3969/j.issn.1000-4440.2018.05.017
文献标志码:
A
摘要:
鸡BF1基因多态性与传染性疾病的抗性有很大关系。鸡BF1基因多态性及功能尚不清楚。本研究直接测定了25个鸡种BF1基因序列,并与从NCBI下载的红色原鸡BF1基因进行了比较,构建系统进化树进行分析。结果显示,在这25个鸡种中共检测到了171个单核苷酸多态性(SNP),其中外显子上发现了108个SNP。这些外显子中SNP位点编码氨基酸166个,其中有58个氨基酸发生变异,氨基酸序列同源性高于核苷酸。外显子4和外显子1的SNP数量最多,占46%以上,第5、第6、第7、第8外显子属于保守区域。核苷酸序列缺失主要发生在外显子1和内含子5上。25个鸡种根据地理位置和气候环境大致可被分为3大类。说明,BF1基因在核苷酸多态性水平上存在较高的变异性,为鸡抗病育种提供了基础。
Abstract:
The polymorphism of chicken BF1 gene is closely related to the resistance of infectious diseases. The polymorphisms and function of chicken BF1 gene have not been clarified. The sequence of BF1 gene of 25 chicken breeds was directly determined and compared with that of the red jungle fowl BF1 gene downloaded from NCBI, then phylogenetic trees were constructed for analysis.The results showed that 171 SNPs were found in total 25 chicken breeds, including 108 SNPs in exon. These SNPs in exons resulted in 58 amino acid variations in the 166 amino acids, the homology of amino acid was higher than that of the nucleotide. The number of SNPs in exon 4 and exon 1 was the highest, accounting for more than 46%, the exon 5, exon6, exon7, exon8 belonged to the conserved region. The deletion of nucleotide sequences occured mainly in exon 1 and intron 5. The 25 chicken species could be roughly divided into three categories based on geographical position and climate environment. In conclusion, a high variability in the level of nucleotide polymorphism was observed in BF1 gene, which provided a basis for chicken disease resistance breeding.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2018-01-26 基金项目:国家科技支撑计划项目(2015BAD03B03);江苏省六大人才高峰项目(2015-NY-019) 作者简介:陈博雯(1993-),女,甘肃靖远人,硕士研究生,主要从事动物遗传资源育种工作。(Tel)15705271693; (E-mail)1061806859@qq.com 通讯作者:常国斌,(Tel)13665241883; (E-mail) passioncgb@163.com
更新日期/Last Update: 2018-11-05