[1]蒙正群,周丽军,罗怡琳,等.2株兔出血症病毒全基因测定与遗传进化分析[J].江苏农业学报,2018,(04):854-861.[doi:doi:10.3969/j.issn.1000-4440.2018.04.020]
　MENG Zheng-qun,ZHOU Li-jun,LUO Yi-lin,et al.Full genetic sequence determination and genetic evolution analysis of two rabbit hemorrhagic disease viruses[J].,2018,(04):854-861.[doi:doi:10.3969/j.issn.1000-4440.2018.04.020]
点击复制

2株兔出血症病毒全基因测定与遗传进化分析()
分享到：

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2018年04期
页码:
854-861
栏目:
畜牧兽医·水产养殖
出版日期:
2018-08-25

文章信息/Info

Title:
Full genetic sequence determination and genetic evolution analysis of two rabbit hemorrhagic disease viruses
作者:
蒙正群1; 周丽军1; 罗怡琳1; 冷依伊1; 张鹏飞1; 王印1; 2; 姚学萍1; 耿毅1; 杨泽晓1
（1.四川农业大学动物医学院，四川温江611130；2.动物疫病与人类健康四川省重点实验室，四川温江611130）
Author(s):
MENG Zheng-qun1; ZHOU Li-jun1; LUO Yi-lin1; LENG Yi-yi1; ZHANG Peng-fei1; WANG Yin1; 2; YAO Xue-ping1; GENG Yi1; YANG Ze-xiao1
(1.College of Veterinary Medicine, Sichuan Agricultural University , Wenjiang 611130, China;2.Key Laboratory of Animal, Disease and Human Health of Sichuan Province，Wenjiang 611130, China)
关键词:
兔出血症病毒; 全基因; 序列; 遗传进化
Keywords:
rabbit hemorrhagic disease virus; complete genome; sequence; genetic evolution
分类号:
S858.291.265
DOI:
doi:10.3969/j.issn.1000-4440.2018.04.020
文献标志码:
A
摘要:
对2株兔出血症病毒（RHDV）SCH04、Sch07进行全基因组序列测定，并进行同源性及遗传进化分析。按照病毒核酸组成，将病毒基因分7段进行RT-PCR扩增，将分段扩增产物分别克隆到pMD-19T载体进行测序，用DNAStar进行拼接，得到全基因组序列；参照 GenBank上登陆的31株RHDV毒株全基因核苷酸序列、VP60基因核苷酸序列以及ORF2 编码基因核苷酸序列对2株病毒进行同源性和遗传进化分析。结果显示，SCH04基因组全长7 439 bp，Sch07基因组全长7 438 bp，2株病毒全基因的核苷酸序列同源性为99.8%，与31株参考毒株全基因的核苷酸序列同源性为 78.3%～97.0%；2株病毒的VP60基因核苷酸序列同源性为99.7%， ORF2编码基因核苷酸序列同源性为99.2%。进化树显示2株病毒同属于抗原变异株RHDVa(GI.1a)基因群，且亲缘关系最近。VP60基因核苷酸序列与ORF2编码基因核苷酸序列均可以作为RHDV遗传进化分析。
Abstract:
The complete genomes of two rabbit hemorrhagic disease viruses（RHDV） SCH04, Sch07 were determinated, and the homologies and genetic evolution were analyzed in the study. The virus′s gene sequences were divided into seven sections for RT-PCR amplification according to the viral nucleic acid, and then the amplification products were cloned into pMD-19T vector for sequencing, and complete genomes were spliced by DNAStar software. The homologies and genetic evolution were analyzed by comparing with 31 strains viral complete genomes, VP60 gene sequences, and ORF2 encoding gene sequences in GenBank. The results showed that fragment length of two strains complete genomes SCH04 and Sch07 were 7 439 bp and 7 438 bp, respectively. The homology of nucleotide sequence between strains complete genomes was 99.8%, and the homologies of two strains with other 31 reference strains complete genomes were 78.3%-97.0%. The homology of VP60 gene sequences between two strains was 99.7% and the homology of ORF2 encoding gene sequences between two strains was 99.2%. Phylogenetic trees results showed the two strains belonged to the antigenic variation RHDVa(GI.1a) group, and genetic relationship was most recently. Both the VP60 gene sequences and ORF2 encoding gene sequences can be used for analyzing evolutionary relationship of RHDV.

参考文献/References:

[1]刘胜江，薛华平，浦伯清，等. 兔的一种新病毒病—兔病毒性出血症[J]. 畜牧与兽医,1984(6): 253-255.
[2]王芳，陈萌萌，宋艳华，等．兔出血症病毒新毒株RHDV2的流行与控制（综述）［J］. 江苏农业科学，2016,44(9):1-3.
[3]XU W Y. Viral haemorrhagic disease of rabbit in China: epidemiology and virus characterization [J]. Revue Scientifique et Technique (International Office of Epizootics), 1991, 10 (2):393-408.
[4]ABRANTES J, VAN DER LOO W, LE P J, et al. Rabbit hemorrhagic disease (RHD) and rabbit hemorrhagic disease virus (RHDV): a review[J]. Vet Res, 2012, 43:12.
[5]LE GALL-RECULE G, ZWINGELSTEIN F, BOUCHER S, et al. Detection of a new variant of rabbit haemorrhagic disease virus in France[J].Vet Rec, 2011,168(5):137-138.
[6]DUARTE M, HENRIQUES M, BARROS S C, et al. Detection of RHDV variant 2 in domestic rabbits in Azores[J].Vet Rec, 2015,176(19):499-500
[7]ABRANTES J, LOPES A M, DALTON K P, et al. New variant of rabbit hemorrhagic disease virus Portugal, 2012–2013[J]. Emerg Infect Dis, 2013 ,19(11):1900-1902.
[8]LE PENDU J, ABRANTES J, BERTAGNOLI S, et al. Proposal for a unified classification system and nomenclature of lagoviruses[J]. J Gen Virol, 2017,98(7):1658-1666.
[9]DALTON K P, NICIEZA I, BALSEIRO A, et al. Variant rabbit hemorrhagic disease virus in young rabbits, Spain[J]. Emerg Infect Dis, 2012,18(12):2009-2012.
[10]MEYERS G, WIRBLICH C, THIEL H J. Genomic and subgenomic RNAs of rabbit hemorrhagic disease virus are both protein-linked and packaged into particles[J]. Virology, 1991, 184(2):677-686.
[11]MARTINEZ-TORRECUADRADA J L, CORTES E, VELA C ,et al. Antigenic structure of the capsid protein of rabbit haemorrhagic disease virus[J]. J Gen Virol, 1998,79(8):1901-1909.
[12]张大鹏,吕宁,符容婕,等. 兔出血症病毒衣壳蛋白VP60的原核表达及多克隆抗体制备[J]. 西北农林科技大学学报(自然科学版),2012,40(3):18-22,28.
[13]李桂黎，任冉阳，胡凌，等. 兔病毒性出血病病毒VP60蛋白基因研究进展[J]. 动物医学进展,2015,36(2):93-96.
[14]陈柳,刘光清,倪征,等. 兔病毒性出血症病毒次要结构蛋白(VP2)的表达和细胞内定位分析[J]. 中国动物传染病学报,2009,17(2):1-7.
[15]CHEN L, LIU G Q, NI Z, et al. Minor structural protein VP2 in rabbit hemorrhagic disease virus downregulates the expression of the viral capsid protein VP60[J]. J Gen Virol, 2009,90(12): 2952-2955.
[16]缪秋红,李传峰,陈宗艳,等. 兔出血症病毒遗传与变异的分子基础[J]. 中国动物传染病学报,2013,21(6):73-79.
[17]MACHIN A, MARTIN ALONSO J M, PARRA F. Identification of the amino acid residue involved in rabbit hemorrhagic disease virus VPg uridylylation [J]. J Biol Chem, 2001,276(30): 27787-27792.
[18]LE GALL G, AMAULD C, BOILLETOT E, et al. Molecular epidemiology of rabbit haemorrhagic disease virus outbreaks in France during 1988 to 1995 [J]. J Gen Virol,1998,79(1):11-16.
[19]FORRESTER N L, TROUT R C, TUMER S L, et al. Unravelling the paradox of rabbit haemorrhagic disease virus emergence, using phylogenetic analysis; possible implications for rabbit conservation strategies[J]. Biological Conservation, 2006,131(2):296-306.
[20]MULLER A, FREITAS J, SILVA E, et al. Evolution of rabbit haemorrhagic disease virus (RHDV) in the European rabbit (Oryctolagus cuniculus) from the Iberian Peninsula[J]. Vet Microbiol,2009, 135(3/4):368-373.
[21]李建文，王红宁，田浪，等. 兔出血症病毒(RHDV)WHNRH株的分离鉴定及基因组全序列的测定与分析[J]. 微生物学报,2006,46(5):720-725.
[22]NEILL J D. Nucleotide sequence of a region of the feline calicivirus genome which encodes picornavirus-like RNA-dependent RNA polymerase, cysteine protease and 2C polypeptides[J]. Virus Res, 1990,17(3):145-160.
[23]BONIOTTI B, WIRBICH C, SIBILIA M, et al. Identification and characterization of a 3C-Like Protease from rabbit hemorrhagic disease virus, a calicivirus[J]. J Virol, 1994, 68(10):6487-6495.
[24]朱杰. VPg 蛋白在兔出血症病毒翻译起始过程中的作用研究[D].北京：中国农业科学院,2015.
[25]NOWOTNY N, BASCUNANA C R, BALLAGI-PORDANY A, et al. Phylogenetic analysis of rabbit haemorrhagic disease and European brown hare syndrome viruses by comparison of sequences from the capsid protein gene[J]. Arch Virol, 1997, 142(4):657-673.
[26]LE GALL G, ZWINGELSTEIN F, LAURENT S, et al. Phylogenetic analysis of rabbit haemorrhagic disease virus in France between 1993 and 2000, and the characterization of RHDV antigenic variants[J].Arch Virol, 2003,148(1): 65-81.
[27]WANG X L, HAO H F, QIU L, et al. Phylogenetic analysis of rabbit hemorrhagic disease virus in China and the antigenic variation of new strains[J].Arch Virol, 2012, 157(8):1523-1530.

相似文献/References:

[1]胡波,盛蓉,宋艳华,等.RHDV VLPs对口蹄疫病毒B细胞表位的展示效果[J].江苏农业学报,2015,(06):1362.[doi:doi:10.3969/j.issn.1000-4440.2015.06.026]
　HU Bo,SHENG Rong,SONG Yan-hua,et al.Presentation of B-cell epitope of foot-and-mouth disease virus in rabbit hemorrhagic disease virus-like particles[J].,2015,(04):1362.[doi:doi:10.3969/j.issn.1000-4440.2015.06.026]
[2]刘维龙,胡波,范志宇,等.N端B细胞表位缺失的兔出血症病毒VP60蛋白的表达及其免疫原性[J].江苏农业学报,2024,(03):508.[doi:doi:10.3969/j.issn.1000-4440.2024.03.013]
　LIU Wei-long,HU Bo,FAN Zhi-yu,et al.Expression of rabbit hemorrhagic disease virus VP60 protein with B cell epitope deletion in N terminus and its immunogenicity in rabbit[J].,2024,(04):508.[doi:doi:10.3969/j.issn.1000-4440.2024.03.013]

备注/Memo

备注/Memo:
收稿日期：2017-11-06 基金项目：国家自然科学基金项目( 31402222) ; “十二五”国家科技支撑计划项目( 2013BAD12B04) ; 四川省科技支撑计划项目( 2016NZ0002) 作者简介：蒙正群（1992-)，女，贵州瓮安人，硕士，主要从事病原分子生物学研究。(E-mail)1193269794@qq.com 通讯作者：杨泽晓，(E-mail)yzxyang2003@126.com
更新日期/Last Update: 2018-09-04