[1]张健,曹成亮,蒋继宏,等.毛泡桐内生放线菌及根际放线菌的筛选、鉴定及代谢产物分析[J].江苏农业学报,2018,(04):775-782.[doi:doi:10.3969/j.issn.1000-4440.2018.04.009]
　ZHANG Jian,CAO Cheng-liang,JIANG Ji-hong,et al.Screening, identification and metabolite analysis of actinomycetes isolated from Paulownia tomentosa and rhizosphere[J].,2018,(04):775-782.[doi:doi:10.3969/j.issn.1000-4440.2018.04.009]
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毛泡桐内生放线菌及根际放线菌的筛选、鉴定及代谢产物分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2018年04期
页码:
775-782
栏目:
植物保护
出版日期:
2018-08-25

文章信息/Info

Title:
Screening, identification and metabolite analysis of actinomycetes isolated from Paulownia tomentosa and rhizosphere
作者:
张健; 曹成亮; 蒋继宏; 鞠秀云
（江苏师范大学生命科学学院，江苏省药用植物生物技术重点实验室，江苏徐州221116）
Author(s):
ZHANG Jian; CAO Cheng-liang; JIANG Ji-hong; JU Xiu-yun
(Key Laboratory of Biotechnology for Medicinal Plant of Jiangsu Province, School of Life Science, Jiangsu Normal University, Xuzhou 221116, China)
关键词:
病原真菌; 放线菌; 毛泡桐; GC-MS分析
Keywords:
pathogenic fungi; actinomycetes; Paulownia tomentosa; gas chromatography-mass spectrometry (GC-MS) analysis
分类号:
S482.2+92
DOI:
doi:10.3969/j.issn.1000-4440.2018.04.009
文献标志码:
A
摘要:
以5种植物病原真菌为靶标菌，采用平板对峙法和生长速率法对分离自河南省新乡市毛泡桐及根际的41株放线菌进行筛选；通过生理生化特征、培养特征、形态特征和基于16S rRNA基因序列的分析，对活性菌株进行鉴定；利用气相色谱-质谱联用技术（GC-MS）对活性菌株发酵液的乙酸乙酯提取物进行分析。结果表明：从41株放线菌中筛选出2株高活性菌株，菌株6002为淀粉酶链霉菌一个亚种（Streptomyces diastaticus subsp. diastaticus），菌株6026为浅紫链霉菌（Streptomyces violascens）。通过GC-MS分析从菌株6002和菌株6026发酵液乙酸乙酯相中分别鉴定出15种和14种化合物。鉴定出的成分中3-羟基-2-甲基-4-吡喃酮、3-异丁基-六氢吡咯并
[1,2-a]吡嗪-1,4-二酮具有很好的生物活性，邻苯二甲酸二丁酯是重要化工原料。
Abstract:
To obtain new antagonistic strains against plant pathogenic fungi, five different plant pathoaenic fungi were used as flag fungi, and 41 actinomycetes strains isolated from Paulownia tomentosa and rhizosphere soil in Xinxiang, Henan province were screened via traditional plate confrontation and growth rate methods. Of these strains, the active strains were identified according to the physiological and biochemical characteristics, cultural characteristics, morphological characteristics, phylogenetic analyses based on 16S rRNA gene sequence. In addition, the liquid fermentation of active actinomycetes was measured by gas chromatography-mass spectrometry (GC-MS). Results showed that two strains with the capability of antifungal activity were screened from the 41 actinomycetes strains, which one strain was identified as Streptomyces diastaticus subsp. diastaticus and the other was Streptomyces violascens. Fifteen compounds from the fermentation broth of strain 6002 and 14 compounds from the fermentation broth of strain 6026 were identified by GC-MS. Of these compounds, 3-hydroxy-2-methyl-4-pyrone and 3-isobutyl hexahydro pyrrolo
[1,2-a] pyrazine-1,4-dione had good biological activity. Dibutyl phthalate was an important raw material for chemical industry.

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备注/Memo

备注/Memo:
收稿日期：2017-11-06 基金项目：江苏师范大学硕士研究生创新科研项目（2016YYB092） 作者简介：张健（1992-），男，安徽宿州人，硕士研究生，研究方向为微生物代谢产物，(E-mail)2442516833@qq.com 通讯作者：鞠秀云, (E-mail)juxiuyun@jsnu.edu.cn
更新日期/Last Update: 2018-09-04