[1]董玲霞,苏一钧,戴习彬,等.基于SSR分子标记的甘薯地上部专用品种遗传多样性分析[J].江苏农业学报,2018,(04):741-746.[doi:doi:10.3969/j.issn.1000-4440.2018.04.004]
　DONG Ling-xia,SU Yi-jun,DAI Xi-bin,et al.Genetic diversity analysis of overground sweetpotato special-purpose varieties based on simple sequence repeats(SSR) markers[J].,2018,(04):741-746.[doi:doi:10.3969/j.issn.1000-4440.2018.04.004]
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基于SSR分子标记的甘薯地上部专用品种遗传多样性分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2018年04期
页码:
741-746
栏目:
遗传育种·生理生化
出版日期:
2018-08-25

文章信息/Info

Title:
Genetic diversity analysis of overground sweetpotato special-purpose varieties based on simple sequence repeats(SSR) markers
作者:
董玲霞; 苏一钧; 戴习彬; 王娇; 唐君; 赵冬兰; 曹清河
（江苏徐淮地区徐州农业科学研究所/农业部甘薯生物学与遗传育种重点实验室，江苏徐州221131）
Author(s):
DONG Ling-xia; SU Yi-jun; DAI Xi-bin; WANG Jiao; TANG Jun; ZHAO Dong-lan; CAO Qing-he
（Xuzhou Institute of Agricultural Sciences of the Xuhuai District of Jiangsu Province/Key Lab of Biology & Genetic Improvement of Sweetpotato, Ministy of Agriculture, Xuzhou 221131, China）
关键词:
菜用甘薯; 观赏甘薯; 菜观兼用甘薯; 遗传多样性; SSR分子标记
Keywords:
vegetable sweetpotato; ornamental sweetpotato;  dual-purpose sweetpotato of edible and ornamental;  genetic diversity; simple sequence repeats(SSR) marker
分类号:
S531.024
DOI:
doi:10.3969/j.issn.1000-4440.2018.04.004
文献标志码:
A
摘要:
为明确国内外甘薯主要地上部专用品种的遗传关系，利用多态性高的30对SSR分子标记对国内主栽的16份材料进行遗传多样性分析。结果显示，30对SSR分子标记共检测出169条多态性条带，每对引物可获得 2～16条多态性条带。基于SSR分子标记进行聚类分析，在遗传相似系数为064处，将16 份材料聚为 3个类群。16份材料的遗传相似系数在0522至0863之间，其中观赏甘薯黄金叶与菜用甘薯材料之间的遗传相似系数较低（0522～0626），与鄂菜薯10号的遗传相似系数最低（为0522）；而菜用甘薯材料之间，薯绿1号和台农71遗传相似系数最高，为0863，遗传差异较小，薯绿1号和鄂菜薯10号遗传相似系数最低，为0522，遗传差异较大。这些信息可为地上部专用品种选育的亲本选配提供参考。
Abstract:
To understand the genetic relationship of main overground sweetpotato special-purpose varieties at home and abroad, 16 varieties were chosen and used for genetic diversity analysis based on simple sequence repeats (SSR) molecular markers. Thirty pairs of markers with high polymorphism were chosen in this study. The results showed that 169 polymorphic loci were detected using the 30 SSR markers, and each primer pair could produce two to 16 polymorphic bands. The 16 tested sweetpotato genotypes could be divided into three groups based on SSR markers when the genetic similarity coefficient was 0.64. The genetic similarity coefficients among 16 accessions varied from 0.522 to 0.863. The genetic similarity coefficients among ornamental sweetpotato Huangjinye and vegetable sweetpotatoes were lower, ranged from 0.522 to 0.626, and the genetic similarity coefficient between Sweet Carolina, and E Caishu No. 10 was the lowest (0.522). Among vegetable sweetpotatoes, the highest genetic similarity coefficient was 0.863 between Shulv No.1 and Tainong No.71, while the lowest genetic similarity coefficient was 0.522 between Shulv No.1 and E Caishu No.10. These showed that the genetic difference was smaller between Shulv No.1 and Tainong No.71, while genetic difference was larger between Shulv No.1 and E caishu No.10. These results would be useful for choosing good parents for overground special-purpose sweetpotato breeding.

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备注/Memo

备注/Memo:
收稿日期：2017-07-01 基金项目：徐州市农业科学院科研基金项目（2015004）；江苏省重点研发技术项目（BK20141144）；徐州市科技创新项目（KC16NG058） 作者简介：董玲霞（1986-），女，山东济南人，硕士，助理研究员，从事甘薯种质创新。(E-mail)donglingxia@jaas.ac.cn 通讯作者：曹清河，(Tel)0516-82028165;(E-mail)cqhe75@yahoo.com
更新日期/Last Update: 2018-09-04