[1]赵凤,王小乐,房伟民,等.外源激素和温度对切花菊侧芽萌发与内源激素含量的影响[J].江苏农业学报,2018,(01):145-151.[doi:doi:10.3969/j.issn.1000-4440.2018.01.021]
　ZHAO Feng,WANG Xiao-le,FANG Wei-min,et al.Effects of hormones and temperature on the lateral buddings and content of endogenous hormones of cut chrysanthemum[J].,2018,(01):145-151.[doi:doi:10.3969/j.issn.1000-4440.2018.01.021]
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外源激素和温度对切花菊侧芽萌发与内源激素含量的影响()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2018年01期
页码:
145-151
栏目:
园艺
出版日期:
2018-02-25

文章信息/Info

Title:
Effects of hormones and temperature on the lateral buddings and content of endogenous hormones of cut chrysanthemum
作者:
赵凤1;  王小乐1;  房伟民1;  张 飞1;  陈发棣1;  管志勇1;  姚建军2;  薛建平2
（1.南京农业大学园艺学院，江苏南京210095；2.上海虹华园艺有限公司，上海200070）
Author(s):
ZHAO Feng1; WANG Xiao-le1; FANG Wei-min1; ZHANG Fei1; CHEN Fa-di1; GUAN Zhi-yong1; YAO Jian-jun2; XUE Jian-ping2
(1.College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China;2.Shanghai Honghua Horticulture Co. Ltd., Shanghai 200070, China)
关键词:
标准切花菊; 侧芽; 萌发; 生长; 激素; 温度
Keywords:
standard cut chrysanthemum; lateral bud; germination; growth; hormones; temperatures
分类号:
S682.1+1
DOI:
doi:10.3969/j.issn.1000-4440.2018.01.021
文献标志码:
A
摘要:
以少侧芽标准型切花菊精の一世为材料，比较了离体条件下不同部位侧芽的萌发特性，以及添加外源NAA、6-BA和不同温度处理对其侧芽萌发及内源激素的影响。结果表明，中部茎段侧芽萌发速度快于上部和下部，长枝率从上部至下部逐步提高。上部侧芽IAA和ABA含量均显著高于中下部；中部ZT/IAA最高，下部次之，上部最低，中下部ZT/ABA、GA/ABA均高于上部。外源NAA浓度的增加使侧芽萌发率和长枝率降低、侧芽的ZT含量显著下降、IAA含量显著上升，而GA和ABA含量没有显著变化，导致 ZT/ABA和ZT/IAA显著降低。0.5 mg/L和1.0 mg/L 6-BA处理均促进了侧芽的萌发，但长枝率明显下降，侧芽的ZT含量显著升高，GA和IAA含量显著下降，导致ZT/IAA显著升高。高温（30 ℃）引起侧芽中ZT含量极显著降低，ABA和GA含量显著升高，使ZT/ABA、IAA/ABA、ZT/IAA以及(GA+IAA+ZT)/ABA显著降低，抑制侧芽萌发生长，而25 ℃侧芽正常萌发生长。综上所述，外源激素和温度能对侧芽的萌发生长产生影响，其中内源激素ZT/IAA的变化起着主导作用。因此，在大量扩繁时应选取萌发率、长枝率高的植株中部或者下部茎段，且在培养基中添加0.5 mg/L的6-BA，25 ℃的培养温度，有助于精の一世的快速扩繁。
Abstract:
With the experimental material of ‘Sei No Issei’, we investigated the characteristics of the axillary buddings at different leaf positions and the effects of exogenous hormones (NAA and 6-BA) and temperatures on the axillary buddings and endogenous hormones of in vitro stem. The results showed that the budding speed of the middle stem was higher than that of the upper and the lower part, and the long branches increased from the upper part to the lower part. The content of IAA and ABA in upper stem was much higher than that in the middle and the lower part. The ZT/IAA value of the middle part was the highest, while that of the upper part was lowest. The values of ZT/ABA and GA/ABA in middle and lower part were higher than those in the upper part. With the increase of the content of exogenous NAA, the lateral budding rate, long branches rate and the endogenous ZT level decreased, the endogenous IAA level raised, and the content of endogenous GA and ABA had no obvious change. As a consequence, the ZT/ABA and ZT/IAA decreased significantly. The lateral buddings rate and the endogenous ZT level increased obviously under the treatment of 0.5 mg/L 6-BA and 1.0 mg/L 6-BA, but the long branches rate and the levels of endogenous GA and IAA reduced significantly, which led to an increase in the ZT/IAA ratio. In addition, high temperature (30 ℃) suppressed lateral buddings long branches, and the levels of endogenous ZT significantly, while promoted the endogenous ABA and GA level, thus the ZT/ABA, IAA/ABA, ZT/IAA and (GA+IAA+ZT)/ABA ratios decreased significantly. Whereas, the axillary buds could sport and grow normally at 25 ℃. In conclusion, middle and lower stems could be selected for a large-scaled propagation for the ‘Sei No Issei’, and the 0.5 mg/L 6-BA in medium and 25 ℃ condition would boost axillary buddings and long branches further.

参考文献/References:

[1]陈俊愉. 园林植物品种分类学[M]. 北京: 高等教育出版社，2001.
[2]肖关丽，杨清辉，李富生，等. 甘蔗腋芽液体培养萌发与内源激素关系[J]. 西南农业大学学报，2001，23(6): 538-541.
[3]DOMAGALSKA M A，LEYSER O. Signal integration in the control of shoot branching [J]. Nature Reviews Molecular Cell Biology，2011，12: 211-221.
[4]蔡铁，徐海成，尹燕枰，等. 外源IAA、GA3和ABA影响不同穗型小麦分蘖发生的机制 [J]. 作物学报，2013，39(10): 1835-1842.
[5]刘凯，余敏，陈海燕，等. 外源IAA和GA3对杨树应拉木形成的影响[J]. 西北农林科技大学学报(自然科学版)，2016，44(3):125-132.
[6]郑传亮，张琦. 6-BA 和 GA对新梨七号芽体抽枝能力的影响[J]. 新疆林业，2013（6）:21-22.
[7]张庆伟，宋春晖，刑利博. 6-BA和 GA4+7 喷施处理及其他措施促进长富 2 号苹果幼苗分枝的效果[J].果树学报，2011，28 (6): 1071-1076.
[8]王东洪. 不同种源山桐子冬芽休眠的温度特性研究[D].郑州：河南农业大学，2012.
[9]李俊香，温超，刘凤栾，等. 温度对切花菊‘深志’侧芽形成的影响[J]. 中国农业大学学报，2014，19(1): 74-79.
[10]侯凯，陈郡雯，申浩，等. 川白芷内源激素的提取纯化和高效液相色谱法同步测定 [J]. 核农学报，2013，27(5): 653-657.
[11]STIRNBERG P，CHATFIELD S P，LEYSER H M O. AXRI bud growth in Arabidopsis acts after lateral bud formation to inhibit lateral bud growth in Arabidopsis [J]. Plant Physiology，1999，121:839-847.
[12]REDDY S K，FINLAYSON S A. Phytochrome B promotes branching in Arabidopsis by suppressing auxin signaling [J]. Plant Physiology，2014，164: 1542-1550.
[13]李春俭. 植物激素在顶端优势中的作用 [J]. 植物生理学通讯，1995，31(6): 401-406.
[14]李春俭. 乙烯在豌豆植株顶端优势中的作用 [J]. 植物生理学报，1997，23(3): 283-287.
[15]NI J，GAO C C，CHEN M S, et al. Gibberellin promotes shoot branching in the perennial woody [J]. Plant Cell and Physiology，2015，56(8): 1655-1666.
[16]高贵宾，潘雁红，吴志庄，等. 美丽箬竹盆栽苗地下茎侧芽萌发特征研究 [J]. 植物科学学报，2016，34(3): 460-468.
[17]MIGUEL L C，LONGNECKER N E，MA Q，et al. Branch development in Lupinus angustifolius L. I. Not all branches have the same potential growth rate [J]. Journal of Experimental Botany，1998，49(320):547-553.
[18]EMERY R J N，LONGNECKER N E，ATKINS C A. Branch development in Lupinus angustifolius L. II. Relationship with endogenous ABA，IAA and cytokinins in axillary and main stem buds [J]. Journal of Experimental Botany，1998，49(320):555-562.
[19]KALOUSEK P，BUCHTOVá D，BALLA J，et al. Cytokinins and polar transport of auxin in axillary pea buds [J]. Acta Universitatis Agricuturae Et Silviculturae Mendelianae Brunensis，2010，54(4):79-87.
[20]刘杨，丁艳锋，王强盛，等. 植物生长调节剂对水稻分蘖芽生长和内源激素变化的调控效应[J]. 作物学报，2011，37(4): 670-676.
[21]蔡铁，徐海成，尹燕枰，等. 外源IAA、GA3和ABA影响不同穗型小麦分蘖发生的机制 [J]. 作物学报，2013，39(10): 1835-1842.
[22]黄坚钦，刘力，章滨森，等. 雷竹地下鞭侧芽内源激素的动态变化研究 [J]. 林业科学，2002，38(3): 38-41.
[23]SCHOELLHORN R K，BARRETT J E，BARTUSKA C A，et al. Elevated temperature affects axillary meristem development in Derzdrarzthema×grandiflorum ‘Improved Mefo’ [J]. Hort Science，2001，36 (6): 1049-1052.
[24]HUH Y J，LIM J H，PARK S K，et al. Endogenous indole-3-acetic acid and transzeatin ribosides in relation to axillary bud formation in standard chrysanthemum [J]. Horticulture Environment and Biotechnology，2011，52(2): 128-132.

备注/Memo

备注/Memo:
收稿日期：2017-06-10 基金项目：国家自然科学基金项目（31372092），上海市种业发展项目[沪农科种字(2016)第1-14号]，江苏省农业科技自主创新基金项目[CX（14）2023] 作者简介：赵凤（1992-），女，湖南常德人，硕士研究生。研究方向为观赏植物遗传育种和栽培技术。（E-mail）459163106@qq.com 通讯作者：房伟民，（E-mail） fangwm@njau.edu.cn
更新日期/Last Update: 2018-03-06