[1]董飚,王健,段修军,等.番鸭IGF-I基因克隆及其在肌肉组织发育中的表达规律[J].江苏农业学报,2018,(01):93-98.[doi:doi:10.3969/j.issn.1000-4440.2018.01.014]
　DONG Biao,WANG Jian,DUAN Xiu-jun,et al.Cloning of insulin-like growth factor-I gene from Muscovy duck and analysis of its expression patterns during muscle development[J].,2018,(01):93-98.[doi:doi:10.3969/j.issn.1000-4440.2018.01.014]
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番鸭IGF-I基因克隆及其在肌肉组织发育中的表达规律()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2018年01期
页码:
93-98
栏目:
畜牧兽医·水产养殖
出版日期:
2018-02-25

文章信息/Info

Title:
Cloning of insulin-like growth factor-I gene from Muscovy duck and analysis of its expression patterns during muscle development
作者:
董飚; 王健; 段修军; 孙国波
（江苏农牧科技职业学院，江苏泰州225300）
Author(s):
DONG Biao;  WANG Jian;  DUAN Xiu-jun;  SUN Guo-bo
(Jiangsu Agri-animal Husbandry Vocational College，Taizhou 225300，China)
关键词:
番鸭; 胰岛素样生长因子-I（IGF-I）; 基因克隆; mRNA表达
Keywords:
Muscovy duck; insulin-like growth factor-I(IGF-I); gene cloning; mRNA expression
分类号:
S834
DOI:
doi:10.3969/j.issn.1000-4440.2018.01.014
文献标志码:
A
摘要:
IGF-I基因在动物肌肉生长过程中起着重要的作用。本试验采用克隆、测序方法获得番鸭IGF-I基因碱基序列，使用生物信息学软件进行序列分析，同时采用实时定量PCR方法分析肌肉发育过程中IGF-I基因的mRNA表达规律。获得了番鸭IGF-I基因563 bp的cDNA序列，包括462 bp的编码区，编码153个氨基酸，与禽类IGF-I基因碱基序列及其编码的氨基酸序列同源性均在98.1%以上，与人和哺乳动物同源性为 76.8%～83.7%。实时荧光定量PCR检测结果显示，在肌肉组织中IGF-I基因mRNA的表达量均表现为第2周龄最高，然后下降，在第4周龄时达到低点，再上升，最后除母鸡腿肌外，其他再次下降，不同性别、不同组织之间下降的周龄有差异。
Abstract:
Insulin-like growth factor I (IGF-I) gene plays an important role in muscle growth of livestock and poultry. In this study, the IGF-I gene of Muscovy duck was cloned and sequenced and its bioinformatics was analyzed. Moreover, quantitative real-time polymerase chain reaction (qRT-PCR) was carried out to investigate mRNA expression patterns of IGF-I gene during muscle growth. A 563-bp cDNA sequence of IGF-I gene of Muscovy duck, composed of a 462-bp coding region and encoding 153 amino acids, was obtained. Its nucleotide and amino acid sequences showed higher than 98.1% homologies with those of other poultry IGF-I genes, and 76.8%-83.7% homologies with those of human and mammals. The qRT-PCR analysis results showed that the IGF-I mRNA expression level in muscle peaked at two weeks of age, then decreased，reached a low point at four weeks of age, then rebounded, and finally decreased rapidly again except the hen leg muscle. The decrease times of expression level varied with gender and tissue.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期：2017-07-22 基金项目：江苏省“青蓝工程”项目[苏教师(2016)15号]；江苏省农业自主创新基金项目[CX(14)2072] 作者简介：董飚（1982-），男，江苏盐城人，硕士，讲师，主要从事家禽资源保护与开发利用工作。（E-mail)dongbiao8201@126.com 通讯作者：王健,（E-mail）tzwjian@126.com
更新日期/Last Update: 2018-03-06