[1]肖婷,陈露,张建华,等.江苏省句容市灰霉病菌对啶酰菌胺的抗药性[J].江苏农业学报,2018,(01):50-55.[doi:doi:10.3969/j.issn.1000-4440.2018.01.007]
　XIAO Ting,CHEN Lu,ZHANG Jian-hua,et al.Boscalid resistance in field populations of Botrytis cinerea in Jurong city of Jiangsu province[J].,2018,(01):50-55.[doi:doi:10.3969/j.issn.1000-4440.2018.01.007]
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江苏省句容市灰霉病菌对啶酰菌胺的抗药性()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2018年01期
页码:
50-55
栏目:
植物保护
出版日期:
2018-02-25

文章信息/Info

Title:
Boscalid resistance in field populations of Botrytis cinerea in Jurong city of Jiangsu province
作者:
肖婷; 陈露; 张建华; 芮东明; 许媛; 吉沐祥; 杨敬辉
（江苏丘陵地区镇江农业科学研究所，江苏句容212400）
Author(s):
XIAO Ting; CHEN Lu; ZHANG Jian-hua; RUI Dong-ming; XU Yuan; JI Mu-xiang; YANG Jing-hui
（Zhenjiang Institute of Agricultural Sciences of the Ning-Zhen Hilly District, Jurong 212400, China）
关键词:
灰霉病菌; 啶酰菌胺; 抗药性
Keywords:
Botrytis cinerea; boscalid; resistance
分类号:
S482.2
DOI:
doi:10.3969/j.issn.1000-4440.2018.01.007
文献标志码:
A
摘要:
为明确江苏省句容市灰霉病菌（Botrytis cinerea）对啶酰菌胺的抗药性水平，本研究2年间采集灰霉病菌122株，采用区分计量法测定其对啶酰菌胺的敏感性，并采用孢子萌发法测定菌株的抑制中浓度（EC50），以确定菌株的抗药性表型，通过对药剂靶标基因（琥珀酸脱氢酶基因）sdhB、sdhC和sdhD的序列分析，确定抗药性分子机理。结果表明，总抗性菌株占42.60%，不同取样点间灰霉病菌对啶酰菌胺的抗性频率有较大差异。本研究根据抗性菌株与敏感菌株EC50的比值（RF）把菌株敏感性表型划分为敏感菌株（RF<2，S）、敏感性下降菌株（2≤RF<3，RS）、低抗菌株（3≤RF<10，LR）、中抗菌株（10≤RF<100，MR）和高抗菌株（RF≥100，HR），其EC50平均值分别为0.044 8 mg/L、0.131 7 mg/L、0.326 1 mg/L、1.255 5 mg/L和11.170 7 mg/L。对9株敏感菌株和所有抗性菌株抗性靶标基因的测序结果进行比对，在所测定的敏感菌株的3个亚基（sdhB、sdhC和sdhD）均未发现氨基酸取代，与敏感菌株相比，所有抗性菌株的sdhB基因在第272位密码子发现有2种类型的突变，即H272R和H272Y突变，sdhC和sdhD基因未发生突变。
Abstract:
To identify the resistance level of Botrytis cinerea on boscalid, 122 strains collected from different areas and plants in 2015 and 2016 were detected for the sensitivity to boscalid using the discriminatory dosage method and the resistance to boscalid represented by EC50 values using spore germination assay. Molecular mechanism of the resistance was identified by the sequence analysis of target genes(succinate dehydrogenase complex，sdhB, sdhC, sdhD). The results showed that resistance frequencies varied in different sampling sites and resistant strains accounted for 42.60%. Based on the RF values (EC50 value of sensitive isolates/EC50 value of resistant isolates). Phenotype of B. cinerea isolates were divided into sensitive（RF<2, S）, reduced sensitive（2≤RF<3, RS）, low resistant（3≤RF<10, LR）, medium resistant（10≤RF<100, MR） and high resistant（RF≥100, HR）, with EC50 values of 0.044 8 mg/L, 0.131 7 mg/L, 0.326 1 mg/L, 1.255 5 mg/L and 11.170 7 mg/L, respectively. No amino acid substitutions were found on the sdhB, sdhC, sdhD of the sensitive strains and sdhC and sdhD of the resistant ones. The resistant isolates presented two types of mutations at codon 272 of sdhB (H272R and H272Y).

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备注/Memo

备注/Memo:
收稿日期：2017-01-20 基金项目：镇江市农业科技支撑项目(NY2015019)；江苏省第五期“333工程”培养基金资助项目（BRA2017149） 作者简介：肖婷（1982-），女，山东兖州人，硕士，副研究员，主要从事植物保护研究。（Tel）0511-80978081；（E-mail）xiaoting826448@163.com 通讯作者：杨敬辉，（E-mail）yjhnn32@126.com
更新日期/Last Update: 2018-03-06