[1]薛洋,闫延华,付敬敬,等.黄芩苷防治雏鸡传染性支气管炎的药理作用[J].江苏农业学报,2017,(05):1082-1092.[doi:doi:10.3969/j.issn.1000-4440.2017.05.019]
　XUE Yang,YAN Yan-hua,FU Jing-jing,et al.Pharmacology of prevention and control of avian infectious bronchitis by baicalin[J].,2017,(05):1082-1092.[doi:doi:10.3969/j.issn.1000-4440.2017.05.019]
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黄芩苷防治雏鸡传染性支气管炎的药理作用()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2017年05期
页码:
1082-1092
栏目:
畜牧兽医·水产养殖
出版日期:
2017-10-30

文章信息/Info

Title:
Pharmacology of prevention and control of avian infectious bronchitis by baicalin
作者:
薛洋1; 闫延华1; 付敬敬1; 刘芳1; 2; 张红英1; 2; 王学兵1; 2
（1.河南农业大学牧医工程学院，河南郑州450000；2.河南省动物性食品安全重点实验室，河南郑州450000）
Author(s):
XUE Yang1; YAN Yan-hua1; FU Jing-jing1; LIU Fang1; 2; ZHANG Hong-ying1; 2; WANG Xue-bing1; 2
（1.College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450000，China;2.Key Laboratory for Animal-derived Food Safety of Henan Province, Zhengzhou 450000，China)
关键词:
黄芩苷; 鸡传染性支气管炎; 药理作用
Keywords:
baicalin; avian infectious bronchitis; pharmacology
分类号:
S858.316.3
DOI:
doi:10.3969/j.issn.1000-4440.2017.05.019
文献标志码:
A
摘要:
为探讨黄芩苷对雏鸡感染传染性支气管炎M41（IBV-M41）的防治效果，将150只12日龄白羽肉鸡随机均分为10组，分别为黄芩苷高、中、低剂量预防组，黄芩苷高、中、低剂量治疗组，双黄连对照组，麻杏石甘颗粒对照组，模型对照组，空白对照组。除空白对照组外其余各组每只鸡于15日龄接种IBV-M41 02 ml。记录雏鸡的采食量及体质量变化、发病情况，测定其生产性能指标、淋巴细胞增殖能力、淋巴细胞亚群及细胞因子浓度。结果表明攻毒后黄芩苷预防和治疗各组料质量比均显著或极显著低于模型对照组；黄芩苷预防高剂量组保护率较高，黄芩苷治疗组治愈率最高可达80%；黄芩苷预防和治疗组淋巴细胞转化能力有明显提升；黄芩苷可以显著提高感染雏鸡CD3+CD8+淋巴细胞比率，使IFN-α浓度长时间维持高水平，并且使CD4+/CD8+的比值及IL-4浓度维持一定的动态平衡。多项检测结果表明黄芩苷抗雏鸡感染IBV-M41兼具预防与治疗作用，其中预防以高剂量
[42 mg/(kg·d)]效果显著，治疗以中剂量
[28 mg/(kg·d)]效果最佳。
Abstract:
To probe into the preventive and control effect of baicalin on avian infectious bronchitis, one hundred and fifty 12-day-old white broiler chickens were randomly divided into 10 groups which were prevention groups with baicalin at high, medium and low dosage, treatment groups with baicalin at a high, medium and low dosage, shuanghuanglian control group, maxingshigan particles control group, model and blank control group. The chickens were infected with avian infectious bronchitis virus M-41 except for those in blank control group. The intake and weight of chickens were recorded and their clinical symptoms were observed. The production performance, lymphocyte proliferation and the concentration of cytokines were detected. The feed/gain ratios of chichens in baicalin-treated groups were lower than those in model control group. The protective rates were high in prevention groups with baicalin at a high dosage and the cure rate was up to 80% in the treatment groups. The lymphocyte proliferation was promoted significantly in baicalin-treated groups. Baicalin enhanced the percentages of CD3+ and CD8+, maintained a high level of IFN-α for a long time, and kept a dynamic balance of CD4+/ CD8+ and IL-4 concentraion. In conclusion, baicalin is capable of prevention and control of avian infectious bronchitis, and a moderate dose at
[28 mg/(kg·d)] for cure and a high dose at
[42 mg/(kg·d)] for prevention were recommended.

参考文献/References:

[1]CAVANAGH D, GELB J. Infectious bronchitis[M]//SAIF Y M, FADLY A M, GLISSON J R, et al. Diseases of Poultry. 12th edition. Ames, Iowa: Blackwell Publishing Professional, 2008：117-135.
[2]何怡宁，李仲林，唐宁 ，等. 桔百颗粒（乐康宁）对雏鸡感染鸡传染性支气管炎的防治效果[J]. 南方农业学报 ，2016，47（9）：1602-1607.
[3]JJ S D W, COOK J K, HM V D H. Infectious bronchitis virus variants: a review of the history, current situation and control measures [J]. Avian Pathol, 2011, 40(3):223-235.
[4]CAVANAGH D. Coronavirus avian infectious bronchitis virus [J]. Vet Res, 2007, 38(2):281-297.
[5]BALESTRIN E, FRAGA A P, IKUTA N, et al. Infectious bronchitis virus in different avian physiological systems-a field study Brazilian poultry flocks [J]. Poult Sci, 2014, 93:1922-1929.
[6]张喜平,田华,程琪辉. 黄芩苷的药理作用研究现状[J]. 中国药理学通报, 2003, 15(11): 1212-1215.
[7]崔岚,袁静,王平全. 黄芩苷药理作用研究进展[J]. 中国医院药学杂志, 2000, 20(11):1127-1129.
[8]郭梦尧. 黄芩苷对小鼠金黄色葡萄球菌性乳腺炎的作用及机制研究[D]. 长春：吉林大学, 2014.
[9]侯艳宁,朱秀媛,程桂芳. 黄芩苷的抗炎机理[J]. 药学学报, 2000, 35(3):161-164.
[10]刘衍季，何小燕，刘晓华, 等. 黄芩苷铜和铝配合物的合成及其生物活性研究[J]. 中国中药杂志，2012，37(9):1296-1306.
[11]郭明，伍周玲，王春歌, 等. 黄芩苷-金属配合物的合成及其抗肿瘤活性研究[J]. 药学学报，2014, 49(3): 337-345.
[12]蔡仙德,谭剑平. 黄芩苷对小鼠细胞免疫功能的影响[J]. 南京铁道医学院学报, 1994, 13(2):65
[13]郑庆,杨宇,刘翠中,等，黄芩苷对慢性间歇性缺氧兔主动脉NF-κB和TNF-α表达的影响[J]. 检验医学与临床, 2017, 14(5):630-636.
[14]王秋菊,杨建省. 黄芩有效成分提取及对传染性支气管炎预防效果的研究[J]. 饲料博览, 2009(12):34-36.
[15]XU G, DOU J, ZHANG L, et al. Inhibitory effects of baicalein on the influenza virus in vivo is determined by baicalin in the serum [J]. Biol Pharm Bull, 2010, 33(2): 238-243.
[16]SUN J Y, LI D L, YAN D, et al. Baicalin inhibits toll-like receptor 2/4 expression and downstream signaling in rat experimental periodontitis[J]. International Immunopharmacology, 2016, 36:86-93.
[17]杨汉春. 动物免疫学[M]. 北京：中国农业大学出版社, 2003:109-112.
[18]刘继平,兰洲,傅强,等. 六味地黄汤相关苷类单体对小鼠脾淋巴细胞增殖作用的研究[J]. 中国中西医结合儿科学, 2013,5(1):5-8.
[19]QIU Y, HU Y L, CUI B A, et al. Immunopotentiating effects of four Chinese herbal polysaccharides administered at vaccination in chickens [J]. Immunlogy, Health and Disease, Poult Sci, 2007, 86(12):2530-2535.
[20]ZHANG Y, YANG S, ZHAO X, et al. Immune enhancement of Taishan Robinia pseudoacacia polysaccharide on recombinant Proteus mirabilis OmpA in chickens [J]. International Immunopharmacology, 2014, 22(1):236-41.
[21]孙文静. 玉屏风多糖的制备及体内外增强免疫活性研究[D]. 泰安：山东农业大学, 2015.
[22]UYANGAA E, CHOI J Y, PATIL A M, et al. Functional restoration of exhausted CD4+ and CD8+ T cells in chronic viral infection by vinegar-processed flos of Daphne genkwa [J]. Comparative Immunology Microbiology & Infectious Diseases, 2015, 39:25.
[23]汪雪,王红宁,阳泰,等. 传染性支气管炎病毒结构蛋白免疫鸡CD4+ CD8+ T淋巴细胞亚群的影响[J]. 中国兽医杂志, 2009, 45(10):13-15.
[24]许银叶,褚夫江,吴玉萍,等. 罗仙子提取物对小鼠脾淋巴细胞的体外增殖CD4+/CD8+比例的影响[J]. 广东医学, 2013, 34(7):985-988.
[25]陈文英,郝艳霜,何欣,等. 蒲公英多糖对雏鸡外周血CD4+和CD8+ T淋巴细胞亚群的影响[J]. 江苏农业科学, 2011, 39(3):265-267.
[26]ZHANG N, BEVAN M J. CD8+ T cells: foot soldiers of the immune system [J]. Immunity, 2011, 35(2):161.
[27]方乐堃,潘凌霄,黎雅婷,等. 复方小柴胡汤对荷EAC鼠IL-2水平和CD4+/CD8+比值的影响[J]. 中国病理生理杂志, 2007, 23(9):1720-1723.
[28]邱妍. 四种中药多糖增强免疫和抗病毒作用及机理研究[D]. 南京：南京农业大学, 2007.
[29]李淑芳,张继东,李英,等. 米糠多糖对免疫抑制鸡外周血CD4+和CD8+ T淋巴细胞亚群的调节作用[J]. 畜牧与兽医, 2009, 41(2):59-62.
[30]孙永科,田占成,王云峰,等. 表达鸡γ-干扰素和传染性支气管病毒S1基因的重组鸡痘病毒疫苗对鸡外周血T淋巴细胞动态分布影响的研究[J]. 中国预防兽医学报, 2006, 28(5):539-543.
[31]黄军,姜国均,周帮会,等. 中药“增免散”对鸡腔上囊细胞凋亡和外周血T细胞亚群的影响[J]. 中国家禽, 2006, 28(17):19-21.
[32]黄蔚,关丽萍,徐世文,等. 硒对氟中毒鸡淋巴细胞亚群及脾淋巴细胞凋亡的影响[J]. 中华地方病学杂志, 2010, 29(2):142-145.
[33]XU Y Y, CHU F J, WU Y P, et al. Effects of housefly maggots extracts on the proliferation and the CD4+/CD8+ of mouser splenic lymphocytes in vitro[J]. Guangdong Medical Journal, 2013,34:985-988.
[34]BELARDELLI F. Role of interferons and other cytokines in the regulation of the immune response [J]. APMIS, 1995, 103(1-6):161-179.
[35]READ S A, DOUGLAS M W. Virus induced inammation and cancer development [J]. Cancer Lett, 2014, 345:174-181.
[36]WANG J Q, JEELALL Y S, FERGUSON L L, et al. Toll-Like receptors and cancer: MYD88 mutation and inammation [J]. Front Immunol, 2014, 5:367.
[37]ARAI K I, LEE F, MIYAJIMA A, et al. Cytokines: coordinators of immune and inflammatory responses [J]. Annu Rev Biochem, 1990, 59:783-836.
[38]ROMAGNANI S. Lymphokine production by human T cells in disease states [J]. Annu Rev Immunol, 1994, 12:227-257.
[39]QU H, YANG L, MENG S, et al. The differential antiviral activities of chicken interferon alpha (ChIFN-alpha) and ChIFN-beta are related to distinct interferon-stimulated gene expression [J]. PLoS One, 2013, 8(3):e59307.
[40]DAI M, WU S, FENG M, et al. Recombinant chicken interferon-alpha inhibits the replication of exogenous avian leukosis virus (ALV) in DF-1 cells[J]. Molecular Immunology, 2016, 76:62-69.
[41]JAROSINSKI K W, JIA W, SEKELLICK M J, et al. Cellular responses in chickens treated with IFN-alpha orally or inoculated with recombinant Marek’s disease virus expressing IFN-alpha [J]. J Interferon Cytokine Res, 2001,21(5): 287-296.
[42]JIANG H, YANG H, KAPCZYNSKI D R. Chicken interferon alpha pretreatment reduces virus replication of pandemic H1N1 and H5N9 avian inuenza viruses in lung cell cultures from different avian species [J]. Virol J, 2011, 8(1):447.
[43]MENG S, YANG L, XU C, et al. Recombinant chicken interferon-alpha inhibits H9N2 avian inuenza virus replication in vivo by oral administration [J]. J Interferon Cytokine Res, 2011, 31(7):533-538.
[44]O′NEILL A M, LIVANT E J, EWALD S J. Interferon a-Induced Inhibition of infectious bursal disease virus in chicken embryo fibroblast cultures differing in Mx genotype [J]. Avian Diseases, 2010, 54(2):802-806.
[45]MO C W, CAO Y C, LIM B L. The in vivo and in vitro effects of chicken interferon alpha on infectious bursal disease virus and Newcastle disease virus infection [J]. Avian Dis, 2001, 45(2):389-399.
[46]PEI J, SEKELLICK M J, MARCUS P I, et al. Chicken interferon type I inhibits infectious bronchitis virus replication and associated respiratory illness [J]. J Interferon Cytokine Res, 2001, 21:1071-1077.
[47]周翠珍,王红梅,齐雪茹,等. 双黄连可溶性粉对鸡传染性支气管炎攻毒实验的效果观察[J]. 中兽医学杂志, 2008(3):13-15.
[48]张素辉,曹国文,陈春林,等. 麻杏石甘散组方提取液对鸡传染性支气管炎病毒影响的研究[J]. 中国畜牧兽医, 2011, 38(10):168-170.
[49]王海凤. 加味麻杏石甘汤防治鸡传染性支气管炎的试验研究[D]. 保定：河北农业大学, 2009.
[50]郭兵,王海凤,庞春酉,等. 中药加味麻杏石甘汤对传染性支气管炎病毒感染雏鸡的保护作用及其机制分析[J]. 中国动物传染病学报, 2010, 18(5):17-22.
[51]黄晓洁,魏刚,张龙,等. 麻杏石甘汤的药理作用和临床应用研究进展[J]. 广东药学院学报, 2014, 30(1):110-114.
[52]刘晓磊,郑继方,罗永江,等. 麻杏石甘汤作用机制及其在兽医临床上的应用[J]. 湖北农业科学, 2012, 51(17):3797-3800.
[53]高艳艳,周德刚,李佑新. 麻杏石甘制剂药理作用及其在兽医临床中的应用[J]. 中国兽药杂志, 2012, 46(10):54-61.
[54]刘淑亚. 麻杏石甘制剂药理作用及其在兽医临床中的应用[J]. 兽医导刊, 2016(8):224-224.

备注/Memo

备注/Memo:
收稿日期：2017-03-24 基金项目：河南省基础与前沿技术研究项目（152300410076）；河南省科技攻关计划项目（152102110048） 作者简介：薛洋（1989-），女，河南开封人，硕士研究生，主要从事中药免疫调节作用研究。(E-mail) xueyangsmile@163.com。闫延华为共同第一作者。 通讯作者：王学兵，(E-mail) xbwang74@163.com
更新日期/Last Update: 2017-11-03