[1]冯辉,聂国嫒,陈曦,等.拟禾谷根结线虫江苏分离群体形态学和分子鉴定[J].江苏农业学报,2017,(04):794-801.[doi:doi:10.3969/j.issn.1000-4440.2017.04.011]
　FENG Hui,NIE Guo-ai,CHEN Xi,et al.Morphological and molecular identification of Meloidogyne graminicola isolated from Jiangsu province[J].,2017,(04):794-801.[doi:doi:10.3969/j.issn.1000-4440.2017.04.011]
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拟禾谷根结线虫江苏分离群体形态学和分子鉴定()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2017年04期
页码:
794-801
栏目:
植物保护
出版日期:
2017-08-30

文章信息/Info

Title:
Morphological and molecular identification of Meloidogyne graminicola isolated from Jiangsu province
作者:
冯辉1; 2; 聂国嫒1; 陈曦1; 张金凤1; 周冬梅1; 魏利辉1; 2
(1.江苏省农业科学院植物保护研究所，江苏南京210014；2.江苏省农业科学院食品质量安全与检测研究所/省部共建国家重点实验室培育基地——江苏省食品质量安全重点实验室，江苏南京210014)
Author(s):
FENG Hui1; 2; NIE Guo-ai1; CHEN Xi1; ZHANG Jin-feng1; ZHOU Dong-mei1; WEI Li-hui1; 2
(1.Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;2.Institute of Food Quality Safety and Detection, Jiangsu Academy of Agricultural Sciences/Key Lab of Food Quality and Safety of Jiangsu Province——State Key Laboratory Breeding Base, Nanjing 210014, China)
关键词:
拟禾谷根结线虫; 形态学特征; ITS-RNA; 28 rRNA; 进化树分析
Keywords:
Meloidogyne graminicola; morphological characteristic; ITS-RNA; 28 rRNA; phylogeny
分类号:
S435.111.4+8
DOI:
doi:10.3969/j.issn.1000-4440.2017.04.011
文献标志码:
A
摘要:
从江苏省泰州地区水稻根结组织中分离获得单一种群的寄生线虫，其雌虫、雄虫和二龄幼虫的de-Man形态学特征与已报道的拟禾谷根结线虫（Meloidogyne graminicola）基本一致。应用ITS-rRNA和28S rRNA D2-D3扩展区分子标记PCR扩增分别获得579 bp和766 bp条带，其序列与M. graminicola台湾、福建、广东、海南等地区部分分离群体的相似度均达99%。贝叶斯系统发育树显示，M. graminicola江苏分离群体与供试的其他种群均位于同一进化分支。M. graminicola江苏分离群体的鉴定结果表明，该线虫从南至北开始向中国其他稻区扩展蔓延，因此亟待采取相关措施以防止水稻根结线虫病的大发生。
Abstract:
A single species of parasitic nematode was isolated from root-knot tissues of rice in the paddy fields of Jiangsu province. The external morphology and de-Man morphometries of the female, male and second instar larvae of nematode were basically identical to other known Meloidogyne graminicola isolates. Molecular markers of ITS-rRNA and 28S rRNA D2-D3 expansion regions were adopted for the identification of a 579-bp and a 766-bp fragments by PCR from the isolated nematode. The sequences were similar to those of M.graminicola from Taiwan, Fujian, Guangdong and Hainan in China. The Bayes phylogenetic tree revealed that the Jiangsu isolate was grouped with Chinese and other populations of M. graminicola into a same clade. Occurrence of M. graminicola in Jiangsu province indicates that this nematode has spread to the rice-growing regions from south to north in China, and related measures should be taken for preventing the outbreak of rice root nematode disease in China.

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备注/Memo

备注/Memo:
收稿日期：2017-02-13 基金项目：国家自然科学基金项目（31401728）；省部共建国家重点实验室培育基地自主研究课题（4911406） 作者简介：冯辉（1985-），男，陕西西安人，博士，助理研究员，主要从事植物线虫学研究。 通讯作者：魏利辉，（Tel）025-84392995；（E-mail）weilihui@jaas.ac.cn
更新日期/Last Update: 2017-09-01