[1]王建,张善磊,赵春芳,等.水稻粒型基因GW2和GS3遗传效应分析[J].江苏农业学报,2017,(04):721-728.[doi:doi:10.3969/j.issn.1000-4440.2017.04.001]
　WANG Jian,ZHANG Shan-lei,ZHAO Chun-fang,et al.Analysis of genetic effects of GW2 and GS3 genes in rice[J].,2017,(04):721-728.[doi:doi:10.3969/j.issn.1000-4440.2017.04.001]
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水稻粒型基因GW2和GS3遗传效应分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2017年04期
页码:
721-728
栏目:
遗传育种·生理生化
出版日期:
2017-08-30

文章信息/Info

Title:
Analysis of genetic effects of GW2 and GS3 genes in rice
作者:
王建1; 2; 张善磊1; 2; 赵春芳2; 赵凌2; 胡庆峰1; 2; 顾明超1; 2; 梁文化2; 路凯2; 张亚东2; 王才林1; 2
（1.南京农业大学农学院/江苏省现代作物生产协同创新中心，江苏南京210095； 2.江苏省农业科学院粮食作物研究所/江苏省优质水稻工程技术研究中心/国家水稻改良中心南京分中心，江苏南京210014）
Author(s):
WANG Jian1; 2; ZHANG Shan-lei1; 2; ZHAO Chun-fang2; ZHAO Ling2; HU Qing-feng1; 2; GU Ming-chao1; 2; LIANG Wen-hua2; LU Kai2; ZHANG Ya-dong2; WANG Cai-lin1; 2
(1.College of Agriculture, Nanjing Agricultural University/Jiangsu Collaborative Innovation Center for Modern Corp Production, Nanjing 210095, China;2.Institute of Food Crops, Jiangsu Academy of Agricultural Sciences/Jiangsu High Quality Rice R&D Center/Nanjing Branch of China National Center for Rice Improvement, Nanjing 210014, China)
关键词:
水稻; GW2基因; GS3基因; 粒型; 籽粒质量
Keywords:
rice; GW2 gene; GS3 gene; grain shape; grain weight
分类号:
S511.032
DOI:
doi:10.3969/j.issn.1000-4440.2017.04.001
文献标志码:
A
摘要:
以大粒品种TD70和小粒品种Kasalath为背景材料，分别对其中的GW2和GS3基因进行了反义和超表达载体的构建和转化。结果表明，Kasalath来源的GW2（GW2K）和GS3 （GS3K）转化TD70的超表达材料籽粒质量均降低，且GW2K的贡献大于GS3K；GW2K和GS3K转化Kasalath的反义表达材料籽粒质量均增加，GW2K的贡献大于GS3K；TD70来源的GW2（GW2T）转化Kasalath的超表达材料千粒质量显著增加，而TD70来源的GS3 （GS3T）转化Kasalath的超表达材料粒型和籽粒质量没有显著变化，说明不同遗传背景中GW2和GS3的基因效应有差异，但同一背景下GW2对籽粒质量的贡献大于GS3。
Abstract:
In this study, GW2 and GS3 genes antisense and overexpression vectors were constructed and transformed with the background materials of big grain variety TD70 and small grain variety Kasalath. The transformed TD70 plants with GW2 (GW2K) or GS3 (GS3K) gene derived from Kasalath plant exhibited reduced grain weight, and GW2K gene contributed more to the reduction than GS3K gene did. The transformed Kasalath plants with GW2K and GS3K antisense genes showed increased grain weight, and GW2K gene contributed more than GS3K gene did. The transformed Kasalath plants with GW2 (GW2T) gene derived from TD70 showed increased 1 000-grain weight while the transformed Kasalath plants with GS3 (GS3T) gene did not, suggesting that the GW2 and GS3 genes exert different genetic effects in different backgrounds and the contribution of GW2 to grain weight in the same background is greater than that of GS3.

参考文献/References:

[1]SONG X J, HUANG W, SHI M, et al. A QTL for rice grain width and weight encodes a previously unknown RING-type E3 ubiquitin ligase[J]. Nature Genetics, 2007, 39(5):623-630.
[2]FAN C, XING Y, MAO H, et al. GS3, a major QTL for grain length and weight and minor QTL for grain width and thickness in rice, encodes a putative transmembrane protein[J]. Theoretical and Applied Genetics, 2006, 112(6):1164-1171.
[3]MAO H, SUN S, YAO J, et al. Linking differential domain functions of the GS3 protein to natural variation of grain size in rice[J]. Proceedings of the National Academy of Sciences of the United States of America, 2010, 107(45):19579-19584.
[4]ZHANG X, WANG J, HUANG J, et al. Rare allele of OsPPKL1 associated with grain length causes extra-large grain and a significant yield increase in rice[J]. Proceedings of the National Academy of Sciences, 2012, 109(52):21534-21539.
[5]LI Y, FAN C, XING Y, et al. Natural variation in GS5 plays an important role in regulating grain size and yield in rice[J]. Nature Genetics, 2011, 43(12):1266-1269.
[6]WENG J, GU S, WAN X, et al. Isolation and initial characterization of GW5, a major QTL associated with rice grain width and weight[J]. Cell Research, 2008, 18(12):1199-1209.
[7]SHOMURA A, IZAWA T, EBANA K, et al. Deletion in a gene associated with grain size increased yields during rice domestication[J]. Nature Genetics, 2008, 40(8):1023-1028.
[8]裔传灯,李玮,王德荣,等. 水稻GW5基因的1212-bp Indel 变异对粒形的影响[J]. 江苏农业学报,2016,32(6):1201-1205.
[9]ISHIMARU K, HIROTSU N, MADOKA Y, et al. Loss of function of the IAA-glucose hydrolase gene TGW6 enhances rice grain, weight and increases yield[J]. Nature Genetics, 2013, 45(6):707-711.
[10]WANG S, WU K, YUAN Q, et al. Control of grain size, shape and quality by OsSPL16 in rice[J]. Nature Genetics, 2012, 44(8):950-954.
[11]谢婷婷，张令蠧，郭伟伟，等．水稻粒型与粒质量的QTL分析［J］．江苏农业科学，2016,44(6):99-103.
[12]高志强,占小登,梁永书,等. 水稻粒形性状的遗传及相关基因定位与克隆研究进展[J]. 遗传, 2011, 33(4):314-321.
[13]曾博虹，孙晓棠，李玲锋，等. 水稻粒重遗传研究进展［J］．南方农业学报，2016，47(12)：2033-2040.
[14]徐正进, 陈温福, 马殿荣,等. 稻谷粒形与稻米主要品质性状的关系[J]. 作物学报, 2004, 30(9):894-900.
[15]刘胜男，张华，柳絮，等. 水稻株高和产量相关性状的QTL定位［J］．山东农业科学，2015,47(4):8-12.
[16]马孟莉，江玲，刘艳红，等．利用籼粳交BIL群体定位水稻谷粒长宽比QTL[J]．江苏农业学报，2015，31(2)：236-240.
[17]钱前. 水稻基因设计育种[M]. 北京:科学出版社, 2007.
[18]陶增. 一对等位WRKY类转录因子基因在水稻抗病反应中的功能分析[D]. 武汉:华中农业大学, 2009.
[19]张亚东. 一种特大粒型水稻粒重遗传的初步研究[C]//中国生物化学与分子生物学会农业分会植物分子育种专业委员会.第五次全国植物分子育种代表大会暨学术交流会论文集.中国生物化学与分子生物学会农业分会植物分子育种专业委员会,2008.
[20]丁丹,张亚东,郑佳,等. 水稻粒长基因GS3和qGL3功能标记的设计及应用[J]. 江苏农业学报, 2014，30(6):1191-1197.
[21]丁丹. 水稻5个粒型相关基因的分子标记开发与效应分析[D]. 南京:南京农业大学, 2014.
[22]王景雪,孙毅. 农杆菌介导的植物基因转化研究进展[J]. 生物技术通报, 1999(1):7-13.
[23]戴争妍,高晓彦,王江,等. 水稻SL基因调控水稻的粒形[J]. 植物生理学报, 2014(8):1159-1166.
[24]张亚东,梁彦丽,郑佳,等. 极端粒形水稻粒宽基因 GW2的序列分析和效应[J]. 中国水稻科学, 2014, 28(6):581-588.
[25]ZHANG Y D, ZHENG J, LIANG Z K, et al. Verification and evaluation of grain QTLs using RILs from TD70 x Kasalath in rice[J]. Genetics & Molecular Research Gmr, 2015, 14(4):14882.
[26]ZHANG Y D, ZHAO Q Y, ZHAO C F, et al.Distribution of seven grain genes and evaluation of their genetic effects on grain traits[J]. Pak J Bot, 2016, 48(4):1073.

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备注/Memo

备注/Memo:
收稿日期：2017-03-09 基金项目：公益性行业科研专项（201603002-5-1）；现代农业产业技术体系建设专项基金项目（CARS-01-47)；江苏省重点研发计划项目（BE2016370） 作者简介：王建（1991-），男，河南南阳人，硕士研究生，主要从事水稻遗传育种研究。(Tel) 15195883032; (E-mail) jianwjaas@hotmail.com 通讯作者：王才林，(E-mail) clwang@jaas.ac.cn
更新日期/Last Update: 2017-09-01