[1]刘昭,于小番,张宇,等.丝氨酸蛋白酶编码基因prtP对副干酪乳杆菌黏附特性的影响[J].江苏农业学报,2017,(03):683-689.[doi:doi:10.3969/j.issn.1000-4440.2017.03.029]
　LIU Zhao,YU Xiao-fan,ZHANG Yu,et al.Adhesion characteristics of Lactobacillus paracasei influenced by serine protease encoding gene prtP[J].,2017,(03):683-689.[doi:doi:10.3969/j.issn.1000-4440.2017.03.029]
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丝氨酸蛋白酶编码基因prtP对副干酪乳杆菌黏附特性的影响()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2017年03期
页码:
683-689
栏目:
加工贮藏·质量安全
出版日期:
2017-06-30

文章信息/Info

Title:
Adhesion characteristics of Lactobacillus paracasei influenced by serine protease encoding gene prtP
作者:
刘昭; 于小番; 张宇; 陈忠琴; 龚威龙; 许慧卿; 崔桂友
（扬州大学旅游烹饪学院，江苏扬州225127）
Author(s):
LIU Zhao; YU Xiao-fan; ZHANG Yu; CHEN Zhong-qin; GONG Wei-long; XU Hui-qing; CUI Gui-you
(College of Tourism and Cuisine, Yangzhou University, Yangzhou 225127, China)
关键词:
副干酪乳杆菌; prtP基因; 基因敲除; 生物学特性; 黏附性
Keywords:
Lactobacillus paracasei; prtP gene; gene knock-out; biological characteristic; adhesion
分类号:
TS201.3
DOI:
doi:10.3969/j.issn.1000-4440.2017.03.029
文献标志码:
A
摘要:
为探讨丝氨酸蛋白酶编码基因prtP对副干酪乳杆菌黏附特性的影响，利用λRed重组系统敲除副干酪乳杆菌的prtP基因，构建副干酪乳杆菌prtP基因缺失突变株，比较突变株与亲本株的表面结构、黏附特性的差异。结果表明，prtP基因缺失突变株的生长速度变慢，疏水性和自聚合性明显下降，抑菌效果无显著性差异，对抗生素的敏感性发生了显著变化，体外对细胞的黏附性显著降低。可见，prtP基因对副干酪乳杆菌的黏附特性十分重要，该基因缺失将改变菌株的生物学特性。
Abstract:
To investigate the effects of serine protease encoding gene prtP on the adhesion of Lactobacillus paracasei, an isogenic prtP mutant was constructed by knocking out prtP gene using λRed recombination system. The surface structure and adhesion characteristics of the mutant were studied. The prtP gene mutant strain showed slower growth rate, decreased hydrophobicity and self condensing ability. The sensitivity to antibiotics of the mutant strain changed in comparison to parental strain, and the adhesion of cells dropped significantly in vitro. In conclusion, the prtP gene is important for the adhesion of L. paracasei, and the deletion could change the biological characteristics of the strain.

参考文献/References:

[1]庄海霁,雷虹,张铁丹,等. 副干酪乳杆菌的应用研究进展[J]. 生物技术通讯，2006(6):989-991.
[2]张硕,张莉力,王玉田. 副干酪乳杆菌对发酵香肠风味的作用研究[J]. 食品工业科技,2014(3):305-307,368.
[3]靳彩娟. 高黏附性乳酸菌的筛选、鉴定及其表面疏水特性研究[D].扬州:扬州大学,2013.
[4]GRANATO D, BERGONZELLI G E, PRIDMERE R D, et al. Cell surface-associated elongation factor Tu mediates the attachment of Lactobacillus johnsonii NCC533 ( La1 ) to human intestinal cells and mucins[J]. Infect Immun, 2004, 72 ( 4 ) : 2160-2169.
[5]王斌,李秋荣,刘放南,等. 1株肠道高黏附性乳杆菌的分离鉴定[J]. 中国生物制品学杂志,2008(6):463-466,474.
[6]MARIE-ANNE VON S, GABRIELE H, MONIKA W, et al. Lactocepin secreted by lactobacillus exerts anti-inflammatory effects by selectively degrading proinflammatory chemokines[J].Cell Host & Microbe,2012,19(4):387-396.
[7]赵迪,曾娟娟,王蕾,等.干酪乳杆菌功能及应用的研究进展[J]. 黑龙江畜牧兽医,2015(3):56-58.
[8]GAO Q Q, XU H Q, WANG X B, et al. RfaH promotes the ability of the avian pathogenic Escherichia coli O2 strain E058 to cause avian colibacillosis [J]. J Bacteriol, 2013, 195(11): 2474-2480.
[9]许慧卿. 禽致病性大肠杆菌E058株lpxL、lpxM和lpxP基因突变株构建及致病性、免疫特性研究[D]. 扬州: 扬州大学, 2013.
[10]任大勇. 益生乳酸杆菌的黏附及免疫调节作用研究[D].长春:吉林大学,2013.
[11]巨晓英. 副干酪乳杆菌的分离鉴定及免疫功能研究[D].天津:天津大学,2009.
[12]SHAKIROVA L, GRUBE M, GAVARE M, et al. Lactobacillus acidophilus La5 and Bifidobacterium lactis Bb12 cell surface hydrophobicity and survival of cells under adverse environmental conditions[J]. J Ind Microbiol Biot,2013,40(1):85-93.
[13]HIROMI K, KOKO M, TAKASHI O, et al. New Lactococcus strain with immunomodulatory activity: enhancement of Th1-type immune response[J]. Microbiology & Immunology, 2004, 48(2): 75-82.
[14]PERDIGON G, NADER DE MACIAS M E, ALVAREZ S, et al. Prevention of gastrointestinal infection using immunobiological methods with milk fermented with Lactobacillus casei and L. acidophilus[J]. Journal of Dairy Research, 1990, 57(2): 255-264.
[15]TONG Z, DONG L, ZHOU L, et al. Nisin inhibits dental caries-associated microorganism in vitro[J]. Peptides,2010,31(11):2003-2008.

备注/Memo

备注/Memo:
收稿日期：2016-11-09 基金项目：国家自然科学基金项目（31101305）；扬州大学大学生创新创业训练计划项目（x20160929） 作者简介：刘昭（1991-），女，山东淄博人，硕士研究生，研究方向为食品微生物学。 通讯作者：许慧卿，（Tel）0514-87978133; （E-mail）yzuxhq@126.com
更新日期/Last Update: 2017-06-29