[1]刘邮洲,陈夕军,尹小乐,等.23株芽胞杆菌及其脂肽类化合物抑菌活性比较[J].江苏农业学报,2017,(03):533-542.[doi:doi:10.3969/j.issn.1000-4440.2017.03.008]
　LIU You-zhou,CHEN Xi-jun,YIN Xiao-le,et al.Comparative analysis of the antagonistic activities of 23 Bacillus spp. strains and their lipopeptide compounds[J].,2017,(03):533-542.[doi:doi:10.3969/j.issn.1000-4440.2017.03.008]
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23株芽胞杆菌及其脂肽类化合物抑菌活性比较()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2017年03期
页码:
533-542
栏目:
植物保护
出版日期:
2017-06-30

文章信息/Info

Title:
Comparative analysis of the antagonistic activities of 23 Bacillus spp. strains and their lipopeptide compounds
作者:
刘邮洲1; 陈夕军2; 尹小乐1; 梁雪杰1; 乔俊卿1; 刘永锋1
（1.江苏省农业科学院植物保护研究所，江苏南京210014；2.扬州大学园艺与植保学院，江苏扬州225009）
Author(s):
LIU You-zhou1; CHEN Xi-jun2; YIN Xiao-le1; LIANG Xue-jie1; QIAO Jun-qing1; LIU Yong-feng1
(1.Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;2.College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, China)
关键词:
芽胞杆菌; 胞外酶; 脂肽类化合物; 抑菌活性
Keywords:
Bacillus spp.; extracellular enzyme; lipopeptide compound; antagonistic activity
分类号:
S482.2+92
DOI:
doi:10.3969/j.issn.1000-4440.2017.03.008
文献标志码:
A
摘要:
为了比较9个种23株芽胞杆菌及其脂肽类化合物抑菌活性差异，本研究以选择性培养基检测各菌株胞外酶分泌物，采用酸沉淀法提取各菌株脂肽类化合物，用对峙生长法分别测定芽胞杆菌及其脂肽类化合物的抑菌活性，高效液相色谱串联质谱(LC-MS)进行脂肽类化合物种类分析。结果表明：23株芽胞杆菌均能产生蛋白酶，枯草芽胞杆菌PTS-394等9个菌株抑菌能力显著，对尖孢镰刀菌、立枯丝核菌、胶孢炭疽病菌和油菜菌核病菌的抑菌带宽分别为 33.4～420 mm、34.6～582 mm、34.6～400 mm和30.1～392 mm。枯草芽胞杆菌JND等6个菌株的脂肽类粗提物抑菌作用显著，对尖孢镰刀菌和立枯丝核菌的抑菌带宽分别为26.2～394 mm和30.5～342 mm。LC-MS检测到有抑菌作用的15株芽胞杆菌的脂肽类化合物种类包括表面活性素家簇、伊枯草素家簇和泛革素家簇。
Abstract:
Bacillus spp. is a kind of biological bacteria effectively preventing and controlling the occurrence and damage of many plant diseases. To evaluate the antagonistic activities of 23 Bacillus strains and their lipopeptide compounds, the selective medium were used to detect the extracellular enzymes produced by 23 strains. The lipopeptide compounds was extracted by acid precipitation method and analyzed by LC-MS. All 23 Bacillus strains produced proteases, and Brevibacillus laterosporu ZR4-5 and B. atrophaeus YL3 produced chitinases besides. Nine Bacillus strains including B. subtilis PTS-394 showed strong and steady antagonistic activities against Fusarium oxysporum,Colletotrichum gloeosporioides, Rhizoctonia solani and Sclerotinia sclerotiorum, with the widths of inhibition band of 33.4-42.0 mm, 34.6-58.2 mm, 34.6-40.0 mm and 30.1-39.2 mm, respectively.The lipopeptide compounds produced by 6 Bacillus strains including B. subtilis JND could inhibit the mycelia growth of F. oxysporum and R. solani, with the width of inhibition band of 26.2-39.4 mm and 30.5-34.2 mm, respectively. Surfactins, iturins and fengycins were detected in the lipopeptide compounds of 15 Bacillus strains by LC-MS analysis.

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备注/Memo

备注/Memo:
收稿日期：2016-11-21 基金项目：江苏省农业科技自主创新基金项目[CX(15)1037] 作者简介：刘邮洲（1975-），女，江苏高邮人，博士，研究员，从事园艺作物病害生物防治研究。（E-mail）shitouren88888@163.com 通讯作者：刘永锋，（E-mail）liuyf@jaas.ac.cn
更新日期/Last Update: 2017-06-29