[1]张杰,单宝来,田永生,等.葡萄HSP17基因的合成与功能分析[J].江苏农业学报,2017,(03):503-509.[doi:doi:10.3969/j.issn.1000-4440.2017.03.004]
　ZHANG Jie,SHAN Bao-lai,TIAN Yong-sheng,et al.Synthesis and functional analysis of HSP17 gene in grape[J].,2017,(03):503-509.[doi:doi:10.3969/j.issn.1000-4440.2017.03.004]
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葡萄HSP17基因的合成与功能分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2017年03期
页码:
503-509
栏目:
遗传育种·生理生化
出版日期:
2017-06-30

文章信息/Info

Title:
Synthesis and functional analysis of HSP17 gene in grape
作者:
张杰1; 单宝来1; 田永生2; 彭日荷2; 姚泉洪2; 汪良驹1
（1.南京农业大学园艺学院，江苏南京210095；2.上海市农业科学院生物技术研究所，上海201106）
Author(s):
ZHANG Jie1; SHAN Bao-lai1; TIAN Yong-sheng2; PENG Ri-he2; YAO Quan-hong2; WANG Liang-ju1
(1.College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China;2.Institute of Biotechnology, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China)
关键词:
葡萄; 小分子热激蛋白; 转基因; 高温胁迫; 苯酚胁迫
Keywords:
grape; small heat shock protein; transgene; high temperature stress; phenol stress
分类号:
S663.1
DOI:
doi:10.3969/j.issn.1000-4440.2017.03.004
文献标志码:
A
摘要:
为了探究葡萄HSP17基因（VvHSP17）的功能，以过量表达VvHSP17的转基因拟南芥幼苗为材料，研究了高温胁迫对植株形态、叶绿素含量、丙二醛含量、脯氨酸含量、超氧化物歧化酶(SOD)活性的影响，同时还观察了苯酚胁迫对植株形态和种子萌发率的影响，并且比较了不同基因型植株对苯酚吸收降解能力的差异。结果表明，与野生型相比，转VvHSP17基因拟南芥抗高温和苯酚胁迫的能力明显增强，逆境胁迫导致的叶绿素含量下降较少，丙二醛含量增幅较小，脯氨酸含量大幅升高，SOD酶活性较高，种子萌发率较高，处理液中苯酚残留量少。说明转VvHSP17基因拟南芥对物理性高温胁迫和化学性苯酚胁迫都有较高的耐受性。
Abstract:
The synthetic VvHSP17 gene was overexpressed in Arabidopsis thaliana to study the morphology of transgenic plants and the changes of chlorophyll content, MDA content, proline content and SOD enzyme activity in response to high temperature. In addition, the differences in phenol uptake, seed germination, and plant growth were compared under phenol stress in transgenic and wild Arabidopsis. Compared with wild type, the tolerances of transgenic Arabidopsis to heat stress and phenol stress were significantly improved. The chlorophyll content in the transgenic plants was less decreased, while the MDA content was less increased. The proline content accumulated more, super oxide dismutase (SOD) activity was higher, seed germination was higher, and the phenol residual in culture liquid was lower. The results confirmed that the VvHSP17 gene increased the heat and phenol tolerance of A. thaliana.

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备注/Memo

备注/Memo:
收稿日期：2016-08-23 基金项目：国家自然科学基金项目（31401820）；江苏省自然科学基金项目（BK20140702）；江苏省苏北科技发展计划项目（BN2012035） 作者简介：张杰（1989-），女，山东淄博人，硕士研究生，从事果树生物技术研究。（E-mail）zhangjieshanyuxi@163.com 通讯作者：汪良驹，（E-mail）wlj@njau.edu.cn
更新日期/Last Update: 2017-06-29