[1]束红梅,郭书巧,巩元勇,等.西红花CCD家族基因序列分析及表达特性[J].江苏农业学报,2017,(02):417-423.[doi:doi:10.3969/j.issn.1000-4440.2017.02.028]
 SHU Hong-mei,GUO Shu-qiao,GONG Yuan-yong,et al.Sequence analysis and expression characteristics of carotenoid cleavage dioxygenases(CCD) genes in Crocus sativus[J].,2017,(02):417-423.[doi:doi:10.3969/j.issn.1000-4440.2017.02.028]
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西红花CCD家族基因序列分析及表达特性()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2017年02期
页码:
417-423
栏目:
园艺
出版日期:
2017-04-30

文章信息/Info

Title:
Sequence analysis and expression characteristics of carotenoid cleavage dioxygenases(CCD) genes in Crocus sativus
作者:
束红梅郭书巧巩元勇蒋璐朱静雯倪万潮
(江苏省农业科学院经济作物研究所,江苏南京210014)
Author(s):
SHU Hong-meiGUO Shu-qiaoGONG Yuan-yongJIANG LuZHU Jing-wenNI Wan-chao
(Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China)
关键词:
西红花CCD家族基因序列分析表达分析
Keywords:
crocinCCD genesequence analysisexpression analysis
分类号:
S567.9;Q786
DOI:
doi:10.3969/j.issn.1000-4440.2017.02.028
文献标志码:
A
摘要:
西红花苷属于脱辅基类胡萝卜素化合物,是中国传统珍贵药材西红花(藏红花)的主要活性成分。CCDs(类胡萝卜素裂解双加氧酶)是催化玉米黄质形成西红花苷等脱辅基类胡萝卜素的重要酶,为了解西红花CCD家族基因的差异,本研究从NCBI数据库中检索获得11个西红花CCD家族基因,对基因序列及表达特性进行分析。结果表明,西红花CsCCD家族基因包括CCD1、CCD4、CCD7、CCD8 4个亚基因家族,各个亚家族的基因序列较为保守、编码的蛋白质三维结构存在差异。通过表达分析发现不同CsCCD基因在不同花器官中的表达特性存在明显差异,这一结果与通过转录组测序得到的CsCCD基因表达水平相符。推测不同CsCCD基因在西红花苷合成过程中的功能存在差异。
Abstract:
Crocin is an apocarotenoid compound, the major active ingredient of saffron (Crocus sativus) which has been cultivated and used as spice and medicinal plant for thousands of years. Carotenoid cleavage dioxygenases (CCDs) are important enzymes that catalyze zeaxantin to generate crocin in Crocus. To understand the difference of CCD genes in Crocus, 11 CsCCD genes searched from NCBI were analyzed for the sequence, structure and expression. The 11 CsCCD genes were divided into four subfamilies(CCD1, CCD4, CCD7, and CCD8) which shared similar conserved domains and different 3D structure of encoded protein. The expressions of CsCCD genes differed in different floral organs (stigma, petal and stamen), which were consistent with the expression patterns of CsCCD gene obtained by the transcription sequencing. It is speculated that different CsCCD genes plays different roles in crocin biosynthesis.

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备注/Memo

备注/Memo:
收稿日期:2016-08-08 基金项目:转基因生物新品种科技重大专项(2016ZX08005-001);江苏省农业科技自主创新基金项目[CX(13)5011] 作者简介:束红梅(1982-),女,江苏射阳人,博士,副研究员,主要从事农业生物技术研究。(Tel)025-84390290;(E-mail)shuhm1982@163.com 通讯作者:倪万潮,(E-mail)nwchao2002@aliyun.com
更新日期/Last Update: 2017-05-02