[1]葛永强,张利娟,杜小姣,等.大叶落地生根组氨醇脱氢酶基因克隆及表达分析[J].江苏农业学报,2017,(02):280-287.[doi:doi:10.3969/j.issn.1000-4440.2017.02.007]
　GE Yong-qiang,ZHANG Li-juan,DU Xiao-jiao,et al.Cloning and expression analysis of histidinol dehydrogenase(KdHDH) gene in Kalanchoe daigremontiana[J].,2017,(02):280-287.[doi:doi:10.3969/j.issn.1000-4440.2017.02.007]
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大叶落地生根组氨醇脱氢酶基因克隆及表达分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2017年02期
页码:
280-287
栏目:
遗传育种·生理生化
出版日期:
2017-04-30

文章信息/Info

Title:
Cloning and expression analysis of histidinol dehydrogenase(KdHDH) gene in Kalanchoe daigremontiana
作者:
葛永强1; 张利娟2; 杜小姣1; 朱敏群1; 黄慧青1; 梁小红3; 钟天秀4
（1.深圳市日昇园林绿化有限公司，广东深圳518040；2.暨南大学深圳旅游学院，广东深圳518053；3.北京林业大学林学院草坪研究所，北京100083；4.华南农业大学林学与风景园林学院草业科学系/广东省草业工程技术研究中心，广东广州510642）
Author(s):
GE Yong-qiang1; ZHANG Li-juan2; DU Xiao-jiao1; ZHU Min-qun1; HUANG Hui-qing1; LIANG Xiao-hong3; ZHONG Tian-xiu4
(1.Shenzhen Risheng Landscape Company Limited, Shenzhen 518040, China;2.Shenzhen Tourism College of Ji’nan University, Shenzhen 518053, China;3.Turfgrass Research Institute, Beijing Forestry University, Beijing 100083, China;4.College of Forestry and Landscape Architecture, South China Agricultural University/Guangdong Engineering Research Center of Grassland Science，Guangzhou 510642, China)
关键词:
大叶落地生根; 组氨醇脱氢酶(HDH); 基因表达
Keywords:
Kalanchoe daigremontiana; histidinol dehydrogenase（HDH）; gene expression
分类号:
Q785
DOI:
doi:10.3969/j.issn.1000-4440.2017.02.007
文献标志码:
A
摘要:
组氨醇脱氢酶将组氨醇氧化成组氨酸，是组氨酸合成途径中的最后一步，调控植物的生长和发育过程。为了更好地理解大叶落地生根中胎生苗调控的分子机制，利用RACE-PCR技术从大叶落地生根中克隆了1个新的组氨醇脱氢酶基因KdHDH。该基因编码的蛋白质具有483个氨基酸残基，分子量为52 130，等电点为5.78，其开放阅读框长度为1 452 bp。KdHDH蛋白与葡萄、拟南芥、蓖麻等植物的蛋白相似性都很高，聚类分析结果表明该蛋白与甜菜和菠菜的遗传距离最近。实时荧光定量PCR分析结果表明，该蛋白基因在大叶落地生根的根中表达量最高，且受渗透胁迫(甘露醇)的诱导下调表达。
Abstract:
Catalyzing histidinol to histidine by histidinol dehydrogenase(HDH) is the final step of his biosynthetic pathway, playing an important role in plant growth and development. To better understand the molecular mechanisms of plantlet formation involved in Kalanchoe daigremontiana, a KdHDH gene was identified using rapid amplification of cDNA end (RACE) PCR. KdHDH gene consists of an ORF of 1 452 bp that was predicted to encode a 483 amino acid residue-long protein of 52 130 with an isoelectric point of 5.78. KdHDH was related most closely to VvHDH, AtHDH and RcHDH, and phylogenetic analysis showed the closest genetic distance with BvHDH and SoHDH. Real-time PCR analysis revealed that KdHDH transcript was expressed highly in root and down-regulated under osmotic stress.

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备注/Memo

备注/Memo:
收稿日期：2016-07-07 基金项目：深圳市知识创新项目（基20160093）；北京林业大学与厦门日懋城建园林建设股份有限公司产学研合作项目 作者简介：葛永强（1986-），男，山东曹县人，本科，主要从事园林绿化工作。（Tel）13410273126；（E-mail）261484794@qq.com 通讯作者：钟天秀,（E-mail）zhongxinbi@163.com
更新日期/Last Update: 2017-05-02