[1]王翔宇,宋玉慧,荆炜,等.鸡源大肠杆菌 BlaCTX鄄M鄄125型 ESBL 基因的定位及遗传环境[J].江苏农业学报,2017,(01):141-145.[doi:10.3969/j.issn.1000-4440.2017.01.022 ]
　WANG Xiang-yu,SONG Yu-hui,JING Wei,et al.The location and genetic environment of blaCTX-M-125 ESBL gene from Escherichia coli in chickens[J].,2017,(01):141-145.[doi:10.3969/j.issn.1000-4440.2017.01.022 ]
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鸡源大肠杆菌 BlaCTX鄄M鄄125型 ESBL 基因的定位及遗传环境()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2017年01期
页码:
141-145
栏目:
畜牧兽医·水产养殖
出版日期:
2017-02-28

文章信息/Info

Title:
The location and genetic environment of blaCTX-M-125 ESBL gene from Escherichia coli in chickens
作者:
王翔宇; 宋玉慧; 荆炜; 朱晓清; 徐倩; 杜向党
（河南农业大学牧医工程学院，河南郑州450003）
Author(s):
WANG Xiang-yu; SONG Yu-hui; JING Wei; ZHU Xiao-qing; XU Qian; DU Xiang-dang
(College of Animal Husbandry and Veterinary, Henan Agricultural University, Zhengzhou 450003, China)
关键词:
β-内酰胺酶; 大肠杆菌; bla CTX-M-125; 接合型质粒
Keywords:
ESBLs; Escherichia coli; blaCTX-M-125; conjugative plasmid
分类号:
S851.34+7.202
DOI:
10.3969/j.issn.1000-4440.2017.01.022
文献标志码:
A
摘要:
采用PCR扩增、测序分析、接合试验、药敏试验、脉冲场凝胶电泳（PFGE）和Southern blot方法，分析了1株兽医临床分离大肠杆菌（sd-3）携带的超广谱β-内酰胺酶（ESBL）基因的状况、分型、定位及遗传环境。结果显示，该株大肠杆菌携带ESBL基因，属于blaCTX-M-9亚群的blaCTX-M-125亚型，系在大肠杆菌中首次检出；接合试验、PFGE和Southern blot证实该基因位于接合型质粒上；blaCTX-M-125基因的上、下游分别存在插入序列ISEcp1和IS903。这表明插入序列和接合型质粒均可在大肠杆菌blaCTX-M-125基因的水平转移中发挥重要作用，有必要在肠杆菌科细菌中开展该基因的监视。
Abstract:
The presence, types, location and genetic environment of extended-spectrum β-lactamases (ESBLs) gene in a Escherichia coli strain (sd-3) isolated from veterinary clinic were investigated by PCR amplification, sequencing analysis, conjugation assay，antimicrobial susceptibility test, pulsed field gel electrophoresis (PFGE) and Southern blot. The results showed the strain carried blaCTX-M-125 ESBLs, belonging to the blaCTX-M-9 subgroup, which was firstly described in Escherichia coli. Conjugation, PFGE and Southern blot confirmed that the gene was located on the conjugative plasmid. The upstream and downstream of the blaCTX-M-125was flanked by insertion sequence ISEcp1 and IS903, respectively. This indicated that both insertion sequence and conjugative plasmid had played an important role in the horizontal transfer of the blaCTX-M-125 ESBL gene. The surveillance of the blaCTX-M-125 in enterobacteriaceae bacteria is necessary.

参考文献/References:

[1]BRADFORD P A. Extended-spectrum beta-lactamases in the 21st century: characterization, epidemiology, and detection of this important resistance threat[J]. Clin Microbiol Rev, 2001,14:933-951.
[2]ZHAO W D, YAN P, GUAN H N, et al. Characterization of CTX-M-type extended-spectrum beta-lactamase in clinical clones of Escherichia coli in Southwest China[J]. J Basic Microbiol, 2014, 54(3): 247-252.
[3]LIVERMORE D M, CANTON R, GNIADKOWSKI M, et al. CTX-M: changing the face of ESBLs in Europe[J]. J Antimicrob Chemother, 2007, 59(2): 165-174.
[4]ECKERT C, GAUTIER V, ARLET G. DNA sequence analysis of the genetic environment of various blaCTX-M genes[J]. J Antimicrob Chemother, 2006,57: 14-23.
[5]YUAN L, LIU J H, HU G Z, et al. Molecular characterization of extended-spectrum beta-lactamase-producing Escherichia coli isolates from chickens in Henan Province, China[J]. J Med Microbiol, 2009,58: 1449-1453.
[6]RIBOT E M, FAIR M A, GAUTOM R, et al. Standardization of pulsed-field gel electrophoresis protocols for the subtyping of Escherichia coli O157: H7, Salmonella, and Shigella for PulseNet[J]. Foodborne Pathog Dis, 2006, 3(1): 59-67.
[7]LARTIGUE M F, POIREL L, NORDMANN P. Diversity of genetic environment of bla(CTX-M) genes[J]. FEMS Microbiol Lett, 2004,234: 201-207.
[8]SALADIN M, CAO V T, LAMBERT T, et al. Diversity of CTX-M beta-lactamases and their promoter regions from Enterobacteriaceae isolated in three Parisian hospitals[J]. FEMS Microbiol Lett, 2002,209: 161-168.
[9]杜向党,焦显芹,莫娟.鸡猪源大肠杆菌CTX-M型ESBLs的分子检测[J].华北农学报, 2009, 24 (2): 90-93.
[10]ZHENG H, ZENG Z, CHEN S, et al.Prevalence and characterisation of CTX-M beta-lactamases amongst Escherichia coli isolates from healthy food animals in China[J]. Int J Antimicrob Agents, 2012,39: 305-310.
[11]KARIM A, POIREL L, NAGARAJAN S, et al. Plasmid-mediated extended-spectrum beta-lactamase (CTX-M-3 like) from India and gene association with insertion sequence ISEcp1[J]. FEMS Microbiol Lett, 2001,201: 237-241.
[12]BONNET R. Growing group of extended-spectrum beta-lactamases: the CTX-M enzymes[J]. Antimicrob Agents Chemother, 2004,48: 1-14.
[13]田勇,郑雪花,张建军,等.鸡源大肠埃希氏菌多药耐药性的监测与分析[J].中国兽药杂志, 2008, 44(1): 18-19.
[14]ZONG Z, PARTRIDGE S R, IREDELL J R. ISEcp1-mediated transposition and homologous recombination can explain the context of bla(CTX-M-62) linked to qnrB2[J]. Antimicrob Agents Chemother, 2010,54: 3039-3042.
[15]TAVAKOLI N P, DEvOST J, DERBYSHIRE K M. Defining functional regions of the IS903 transposase[J]. J Mol Biol, 1997, 274: 491-504.

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备注/Memo

备注/Memo:
收稿日期：2016-03-30 基金项目：河南省高等学校重点科研项目(14A230008) 作者简介：王翔宇（1989-），女，河南开封人，硕士，主要从事细菌耐药性研究。（E-mail）jichushouyi402@163.com 通讯作者：杜向党,（E-mail）xddu@henau.edu.cn
更新日期/Last Update: 2017-04-12