[1]闫圆圆,曾爱松,宋立晓,等.结球甘蓝幼苗耐热性鉴定方法及耐热生理[J].江苏农业学报,2016,(04):885-890.[doi:10.3969/j.issn.100-4440.2016.04.027]
 YAN Yuan-yuan,ZENG Ai-song,SONG Li-xiao,et al.Identification of heat tolerance in cabbage seedlings and heat-tolerant physiology[J].,2016,(04):885-890.[doi:10.3969/j.issn.100-4440.2016.04.027]
点击复制

结球甘蓝幼苗耐热性鉴定方法及耐热生理()
分享到:

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2016年04期
页码:
885-890
栏目:
园艺
出版日期:
2016-10-31

文章信息/Info

Title:
Identification of heat tolerance in cabbage seedlings and heat-tolerant physiology
作者:
闫圆圆曾爱松宋立晓严继勇
江苏省农业科学院蔬菜研究所,江苏 南京 210014
Author(s):
YAN Yuan-yuanZENG Ai-songSONG Li-xiaoYAN Ji-yong
Institute of Vegetable Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
关键词:
甘蓝 耐热性鉴定 相对电导率 MDA含量 游离脯氨酸含量 SOD活性
Keywords:
cabbage heat resistance identification relative electric conductivity MDA content proline content SOD activity
分类号:
S635.1
DOI:
10.3969/j.issn.100-4440.2016.04.027
文献标志码:
A
摘要:
为了筛选出一套适用于甘蓝苗期耐热性鉴定的方法,以耐热性不同的6份甘蓝材料为试验材料,通过采用不同的胁迫温度、胁迫时间和胁迫苗龄对甘蓝苗期耐热性进行评价,并测定不同材料的生理指标。结果显示,4~5片真叶的甘蓝幼苗在38.0 ℃/29.0 ℃(昼/夜)人工模拟气候箱中处理3 d,可以准确评价不同类型甘蓝材料的耐热性差异。高温处理3 d后,甘蓝幼苗相对电导率大幅升高,其中耐热材料启夏的增幅小于热敏感材料W410。随着高温胁迫时间的延长,启夏和W410的MDA含量和游离脯氨酸含量呈升高的趋势,且两者间差异达显著水平,两种类型材料的SOD活性均呈现出先上升后下降趋势,其中启夏的SOD活性始终高于W410。POD活性变化与耐热性无明显相关性。因此,相对电导率、MDA含量、游离脯氨酸含量和SOD活性均可以作为甘蓝苗期耐热性鉴定的间接指标。
Abstract:
To develop a method for identifying heat tolerance at seedling stage,six cabbage varieties or inbred lines with different heat tolerances were evaluated for the optimal temperature, time duration and seedling age of high temperature stress. The cabbage seedlings with 4-5 true leaves inside artificial climate box under 38.0 ℃ / 29.0 ℃ (d/n) for 3 d was favorable for heat tolerance identification. After high temperature treatment for 3 d, the relative conductivity of cabbage seedlings dramatically increased, and the increment was bigger in heat-sensitive materials than that in heat-tolerant materials. As high temperature stress lasted, the contents of MDA and proline in both heat-tolerant and-sensitive material climbed up, and the SOD activities declined following an initial rising. The SOD activity in heat-tolerant variety was higher than that in heat-sensitive variety. The POD activity was variety-independent. In sum, relative conductivity, MDA content, proline content activity could be used as indirect indicators for heat tolerance identification of cabbage seedlings.

参考文献/References:

[1]王庆彪,方智远,杨丽梅,等.中国甘蓝育成品种系谱分析[J].园艺学报,2013,40(5):869-886.
[2]陈碧华.温度处理对甘蓝幼苗耐热性的影响研究[D].重庆:西南农业大学,2004.
[3]宋立晓,高兵,张边江,等.甘蓝耐寒性生理生化指标研究[J].江苏农业学报,2009,25(6):1341-1346.
[4]BERRY J, BJORKMAN O. Phptosynthetic response and adaptation to temperature in high plants[J]. Annu Rev Plant Physiol,1980, 31:491-543.
[5]康俊根,翟依仁.甘蓝耐热性遗传效应分析[J].华北农学报,2003,18(3): 93-95.
[6]候晟灿.早秋耐热甘蓝新品种的选育[D].武汉:华中农业大学,2014.
[7]康俊根,翟依仁,张京社,等.甘蓝耐热性鉴定方法[J]. 中国蔬菜,2002(1):4-7.
[8]阳燕娟,来可可,陈文明,等. 砧用葫芦种质资源耐热性评价[J]. 南方农业学报, 2015,46(6):1047-1052.
[9]韦志扬,张力,陆杰思,等. 高温胁迫下不同皮色西瓜耐热性评价研究[J]. 南方农业学报, 2014,45(4):565-569.
[10] 王学奎.植物生理生化实验原理和技术[M].2版.北京:高等教育出版社,2006.
[11] 李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000.
[12] 邓耀华,程萍,候晟灿,等.早秋甘蓝品种的耐热性鉴定[J].长江蔬菜,2013(20):18-20.
[13] 张景云,赵晓东,王建新,等.小白菜耐热性鉴定及其耐热性分析[J].核农学报,2014,28(1):146-153.
[14] 吴国胜,曹婉虹,王永健,等. 细胞膜热稳定性及保护酶和大白菜耐热性的关系[J].园艺学报,1995,22(4): 353-358.
[15] 司家钢,孙日飞,吴飞燕,等.高温胁迫对大白菜耐热性相关生理指标的影响[J].中国蔬菜,1995(4):4-6.
[16] 马晓娣,王丽,汪矛,等.不同耐热性小麦品种在热锻炼和热胁迫下叶片相对电导率及超微结构的差异[J].中国农业大学学报,2003,8(5):4-8.
[17] HSU Y T,KAO C H.Cadmium-induced oxidative damage in rice leaves is reduced by polyamines[J].Plant Soil,2007,291:27-37.
[18] MISHRA Y,BHARGAVA P,RAI L C.Different induction of enzymes and antioxidants of the antioxidative defence system in Anabaena doliolum exposed to heat stress[J].J Therm Biol,2005,30:524-531.
[19] 马宝鹏,巩振辉.辣椒幼苗对高温胁迫的生长生理响应[J].西北农林科技大学学报:自然科学版,2013,41(10):113-115.
[20] 李成琼,宋洪元,雷建军.甘蓝耐热性鉴定研究[J].西南农业大学学报,1998,20(4):298-301.
[21] WAHID A,GELANI S,ASHRAF M,et al.Heat tolerance in plants:an overview [J].Environmental and Experimental Botany,2007,61:199-223.
[22] 娄丽娜,蒋立春,孙俪,等.持续高温胁迫对不结球白菜生理指标的影响[J]. 江苏农业科学,2015,43(6):161-164.
[23] 叶凡,侯喜林,袁建玉.高温胁迫对不结球白菜幼苗抗氧化酶活性和膜质过氧化作用的影响[J].江苏农业学报,2007,23(2):154-156.
[24] GALLEGO S M,BENAVIDES M P,TOMARO M L.Effect of heavy metal ion excess on sunflower leaves: evidence for involvement of oxidative stress [J].Plant Sci,1996,121:151-159.
[25] LIDON F C, TEIXEIRA M G. Oxy radicals’production and control in the chloroplast of Mn-treated rice[J] Plant Sic,2000,152:7-15.
[26] 叶陈亮,柯玉琴,陈伟. 大白菜耐热性生理研究 Ⅲ.酶性和非酶性活性氧清除能力与耐热性[J]. 福建农业大学学报,1997,26(4):499-501.
[27] 苏小俊,袁希汉,徐海.大白菜耐热性和过氧化物酶活性与热害指数的相关性[J].江苏农业学报,2007,23(5):456-458.
[28] 胡俏强.不结球白菜耐热性鉴定方法及其耐热基因片段克隆研究[D].南京:南京农业大学,2011:52-56.

相似文献/References:

[1]戴忠良,陈丽,山溪,等.甘蓝晚抽薹基因BoFLC3克隆、序列分析和亚细胞定位[J].江苏农业学报,2018,(06):1324.[doi:doi:10.3969/j.issn.1000-4440.2018.06.018]
 DAI Zhong-liang,CHEN Li,SHAN Xi,et al.Cloning, sequence analysis and subcellular localization of Brassica oleraceaBoFLC3 gene[J].,2018,(04):1324.[doi:doi:10.3969/j.issn.1000-4440.2018.06.018]
[2]秦文斌,戴忠良,山溪,等.甘蓝冷胁迫相关基因BobHLH18克隆与表达分析[J].江苏农业学报,2019,(01):149.[doi:doi:10.3969/j.issn.1000-4440.2019.01.022]
 QIN Wen-bin,DAI Zhong-liang,SHAN Xi,et al.Molecular cloning and expression analysis of cold stress-related gene BobHLH18 in cabbage (Brassica oleracea var. capitata L.)[J].,2019,(04):149.[doi:doi:10.3969/j.issn.1000-4440.2019.01.022]
[3]郭峰,孙莹,安容慧,等.LED绿光处理对甘蓝采后品质及抗氧化活性的影响[J].江苏农业学报,2023,(02):489.[doi:doi:10.3969/j.issn.1000-4440.2023.02.022]
 GUO Feng,SUN Ying,AN Rong-hui,et al.Effects of green light-emitting diode light treatment on the quality and antioxidant capacity of postharvest green cabbage (Brassica oleracea var. capitata L.)[J].,2023,(04):489.[doi:doi:10.3969/j.issn.1000-4440.2023.02.022]
[4]刘志刚,余方伟,张伟,等.甘蓝黑斑病病原菌鉴定及其对杀菌剂的敏感性[J].江苏农业学报,2023,(04):947.[doi:doi:10.3969/j.issn.1000-4440.2023.04.004]
 LIU Zhi-gang,YU Fang-wei,ZHANG Wei,et al.Identification and fungicide sensitivity of the pathogen causing black spot on Brassica oleracea var. capitata L.[J].,2023,(04):947.[doi:doi:10.3969/j.issn.1000-4440.2023.04.004]

备注/Memo

备注/Memo:
收稿日期:2015-12-21基金项目:江苏省农业科技支撑计划项目(BE2013429);江苏省农业科技自主创新基金项目[CX(15)1015]作者简介:闫圆圆(199-),女,山东省滨州人,硕士研究生,主要从事结球甘蓝遗传与生物技术育种研究。(Tel)15951971276;(E-mail)1126928018@qq.com通讯作者:严继勇,(Tel)13951890164;(E-mail)yjyqr@163.com
更新日期/Last Update: 2016-11-01