[1]李余,石辉,段保正,等.不同植物叶片角质膜分离方法对角质膜水分渗透性的影响[J].江苏农业学报,2016,(04):769-773.[doi:10.3969/j.issn.100-4440.2016.04.009]
　LI Yu,SHI Hui,DUAN Bao-zheng,et al.Water permeability of plant cuticular membranes isolated with different chemical agents[J].,2016,(04):769-773.[doi:10.3969/j.issn.100-4440.2016.04.009]
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不同植物叶片角质膜分离方法对角质膜水分渗透性的影响()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2016年04期
页码:
769-773
栏目:
遗传育种·生理生化
出版日期:
2016-10-31

文章信息/Info

Title:
Water permeability of plant cuticular membranes isolated with different chemical agents
作者:
李余; 石辉; 段保正; 魏小芳; 蒋子银
西安建筑科技大学环境与市政工程学院，陕西 西安 710055
Author(s):
LI Yu; SHI Hui; DUAN Bao-zheng; WEI Xiao-fang; JIANG Zi-yin
School of Environmental and Municipal Engineering, Xi’an University of Architecture and Technology, Xi’an 710055，China)
关键词:
角质膜; 分离方法; 微蒸腾室; 水分渗透速率
Keywords:
cuticular membrane; isolation; microtranspiration chamber; water permeability
分类号:
S688
DOI:
10.3969/j.issn.100-4440.2016.04.009
文献标志码:
A
摘要:
为了探究草酸/草酸铵、果胶酶/纤维素酶、氯化锌/盐酸、三氧化铬4种叶片角质膜分离方法对角质膜水分渗透性的影响，以大叶女贞（Ligustrum lucidum Ait.）、大叶黄杨（Euonymus japonicus）、海桐（Pittosporum tobira）叶片上表皮无气孔角质膜为材料，利用扫描电镜和微蒸腾室分别对角质膜结构与水分渗透速率进行研究。结果表明，与完整叶片对照相比，草酸/草酸铵法剥离出的3种叶片角质膜结构较为完整，且水分渗透速率与完整叶片相近，为最佳的叶片角质膜剥离方法；植物叶片质地的差异影响角质膜剥离方法的选择，纸质叶可优先选择氯化锌/盐酸法、草酸/草酸铵法，厚革质叶片角质膜的剥离可优先选择草酸/草酸铵法。
Abstract:
Four chemical agents, oxalic acid / ammonium oxalate solution, cellulase/pectinase solution, zinc chloridehydrochloric acid solution and chromium trioxide solution were applied to isolate the upper astomatous cuticular membranes of Ligustrum lucidum Ait, Euonymus japonicus and Pittosporum tobira  to study the structure of plant cuticles with scanning electron microscope and the speed of water infiltration of cuticular membranes in a microtranspiration chamber. Compared with unisolated leaf, the structure of plant cuticles was relatively integrated when isolated with oxalic acid/ammonium oxalate solution, and the rate of water infiltration was close to that of complete leaf, which made oxalic acid/ammonium oxalate solution the preferred agent for isolation. Leaf texture was associated with the selection of isolation agents. The zinc chloridehydrochloric acid solution and oxalic acid/ammonium oxalate solution were the choice for cuticular membranes isolation of papery leaves, and oxalic acid/ammonium oxalate solution was the choice for thick and leathery leaves.

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备注/Memo

备注/Memo:
收稿日期：2015-11-19基金项目：陕西省自然科学基金重点项目（2014JZ011）；林业公益性行业科研专项（201304301-05）作者简介：李余（1989-），男，四川广安人，硕士研究生，研究方向为环境生态。(E-mail)liyu_data@163.com
更新日期/Last Update: 2016-11-01