[1]祝昊丹,倪艳秀,茅爱华,等.丝氨酸/苏氨酸激酶对猪链球菌2型应激和毒力的影响[J].江苏农业学报,2016,(03):593.[doi:10.3969/j.issn.1000-4440.2016.03.017]
 ZHU Hao-dan,NI Yan-xiu,MAO Ai-hua,et al.The impact of serine/threonine kinase on stress response and virulence of Streptococcus suis type 2[J].,2016,(03):593.[doi:10.3969/j.issn.1000-4440.2016.03.017]
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丝氨酸/苏氨酸激酶对猪链球菌2型应激和毒力的影响()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2016年03期
页码:
593
栏目:
畜牧兽医·水产养殖
出版日期:
2016-06-30

文章信息/Info

Title:
The impact of serine/threonine kinase on stress response and virulence of Streptococcus suis type 2
作者:
祝昊丹 倪艳秀 茅爱华 周俊明 俞正玉 吕立新 何孔旺
江苏省农业科学院兽医研究所,农业部兽用生物制品工程技术重点实验室,国家兽用生物制品工程技术研究中心,江苏 南京 210014
Author(s):
ZHU Hao-dan NI Yan-xiu MAO Ai-hua ZHOU Jun-ming YU Zheng-yu LYU Li-xin HE Kong-wang
Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences/Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture/National Center for Engineering Research of Veterinary Bio-products, Nanjing 210014, China
关键词:
猪链球菌2型 丝氨酸/苏氨酸激酶 应激 毒力
Keywords:
Streptococcus suis type 2 serine/threonine kinase stress tolerance virulence
分类号:
S858.285.1+1
DOI:
10.3969/j.issn.1000-4440.2016.03.017
文献标志码:
A
摘要:
为阐明丝氨酸/苏氨酸激酶(STK)对猪链球菌2型强毒株的应激耐受和毒力的影响,比较了亲本株SS2-1及其丝氨酸/苏氨酸激酶基因缺失株Δstk在各种应激环境(热、酸、氧化应激和高渗应激)中的生长能力,利用HEp-2细胞模型比较各菌株的黏附能力,比较各菌株在猪全血中的存活能力,利用仔猪模型评价STK基因缺失前后细菌的毒力差异。结果表明,丝氨酸/苏氨酸激酶基因缺失后,细菌在各种应激环境中的生存能力明显减弱,对HEp-2细胞的黏附能力减弱,在猪全血中的存活能力显著降低,对仔猪的致病力明显降低。此外,缺失株Δstk在仔猪体内各器官的定殖能力也显著降低。说明丝氨酸/苏氨酸激酶与猪链球菌的应激和毒力密切相关。
Abstract:
To investigate the effect of serine/threonine kinases(STK)in Streptococcus suis type 2 virulent strain on stress tolerance and virulence, the growth characteristics of wild type SS2-1 and STK-deletion strain Δstk were compared under different stress conditions(high temperature, acidic pH, oxidative stress, and high osmolarity.)in vitro. The Δstk mutant showed defects in their ability to grow under various stress conditions, compared to the wild-type strain. The Δstk exhibited a significant decrease in HEp-2 cell adhesion, and a reduced survival rate in whole blood. In the piglet model, the Δstk also showed a weak virulence compared to wild-type strain. The in vivo colonization experiments revealed that the mutant strain Δstk displayed reduced bacterial colonization in the tissues of piglets. These results suggest that the STK is closely related to the stress response and virulence of SS2.

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备注/Memo

备注/Memo:
收稿日期:2015-10-26
基金项目:国家博士后基金项目(2012M521026); 国家自然科学基金项目(31302114); 公益性行业(农业)科研专项经费项目(201303041); 江苏省自主创新探索性研究项目[CX(14)5042]
作者简介::祝昊丹(1983-),女,黑龙江哈尔滨人,博士,主要从事人畜共患病防控技术研究。(Tel)025-84390988;(E-mail)zhuhaodan0311@126.com
通讯作者:何孔旺,(Tel)025-84390748;(E-mail)kwh2003@263.net
更新日期/Last Update: 2016-06-30