[1]马百全,刘吉英,李平华,等.苏淮猪BMP4基因克隆、组织表达及真核表达[J].江苏农业学报,2016,(03):581.[doi:10.3969/j.issn.1000-4440.2016.03.015]
　MA Bai-quan,LIU Ji-ying,LI Ping-hua,et al.Cloning, expression profile and eukaryotic expression of BMP4 gene in Suhuai swine[J].,2016,(03):581.[doi:10.3969/j.issn.1000-4440.2016.03.015]
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苏淮猪BMP4基因克隆、组织表达及真核表达()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2016年03期
页码:
581
栏目:
畜牧兽医·水产养殖
出版日期:
2016-06-30

文章信息/Info

Title:
Cloning, expression profile and eukaryotic expression of BMP4 gene in Suhuai swine
作者:
马百全1;  刘吉英1;  李平华1; 2;  潘增祥1;  吴 艳3;  王钧顺3;  刘红林1;  李齐发1
1.南京农业大学动物科技学院,江苏 南京 210095; 2.南京农业大学淮安研究院,江苏 淮安 223005; 3.淮阴种猪场,江苏 淮安 223322
Author(s):
MA Bai-quan1;  LIU Ji-ying1;  LI Ping-hua1; 2;  PAN Zeng-xiang1;  WU Yan3;  WANG Jun-shun3;  LIU Hong-lin1;  LI Qi-fa1
1.College of Animal Science and Technology,Nanjing Agricultural University, Nanjing 210095,China; 2.Huaian Academy of Nanjing Agricultural University,Huai’an 223005,China; 3.Huaiyin Pig Breeding Farm, Huai’an 223322,China
关键词:
苏淮猪;  BMP4;  表达模式;  真核表达
Keywords:
Suhuai swine;  BMP4;  expression pattern;  eukaryotic expression
分类号:
S828
DOI:
10.3969/j.issn.1000-4440.2016.03.015
文献标志码:
A
摘要:
为了解苏淮猪BMP4基因的序列特征和表达特征,本研究采用克隆测序技术获得苏淮猪BMP4基因编码区全序列,利用生物信息学方法分析BMP4基因的序列特征,采用RT-PCR技术检测BMP4基因在苏淮猪不同组织中的表达情况,构建苏淮猪BMP4基因真核表达载体并利用Western blot技术检测BMP4真核表达载体的表达情况。结果显示苏淮猪BMP4基因编码区序列长度为1 230 bp,编码409个氨基酸,与其他哺乳动物氨基酸序列的一致性均在97%以上; 苏淮猪BMP4编码蛋白含有典型的TGF-β前肽和TGF-β样结构域; BMP4基因在苏淮猪卵巢组织中高表达; 成功构建苏淮猪BMP4基因真核表达质粒pcDNA3.1-BMP4,转染后可显著上调猪卵巢颗粒细胞BMP4蛋白表达水平。
Abstract:
The coding sequence of Suhuai pig BMP4 gene obtained by cloning and sequencing was analyzed by bioinformatics and the expression patterns were identified by RT-PCR. The full length coding region of BMP4 was 1 230 bp in length, encoding a protein with 409 amino acids which shared more than 97% similarities with other mammals. BMP4 protein of Suhuai pig contained TGF-β propeptide and TGF-β domain. RT-PCR assays showed that BMP4 is widely expressed in tissues and organs. BMP4 protein level was upregulated in porcine ovarian granulosa cells transfected with recombinant plasmid pcDNA3.1-BMP4. The results laid a foundation for investigation on the role of BMP4 in porcine follicular development.

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备注/Memo

备注/Memo:
收稿日期:2016-02-25
基金项目:江苏省农业科技自主创新基金项目[SCX(12)2097]
作者简介::马百全(1989-),男,河南濮阳人,硕士研究生,主要从事动物分子遗传研究。(E-mail)mbq8908@126.com
通讯作者:李齐发,(E-mail)liqifa@njau.edu.cn
更新日期/Last Update: 2016-06-30