[1]姜华年,丁 婷.厚朴内生真菌HPFJ3对小麦全蚀病的生防作用[J].江苏农业学报,2016,(02):285-292.[doi:10.3969/j.issn.1000-4440.2016.02.008]
 JIANG Hua-nian,DING Ting.Biocontrol effect of endophytic fungus HPFJ3 from Magnolia officinalis on wheat take-all disease[J].,2016,(02):285-292.[doi:10.3969/j.issn.1000-4440.2016.02.008]
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厚朴内生真菌HPFJ3对小麦全蚀病的生防作用()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2016年02期
页码:
285-292
栏目:
植物保护
出版日期:
2016-03-20

文章信息/Info

Title:
Biocontrol effect of endophytic fungus HPFJ3 from Magnolia officinalis on wheat take-all disease
作者:
姜华年1 丁 婷2
1.丽水学院生态学院,浙江 丽水 323000; 2.安徽农业大学,安徽 合肥 230036
Author(s):
JIANG Hua-nian1 DING Ting2
1.College of Ecology, Lishui University, Lishui 323000, China; 2.Anhui Agricultural University, Hefei 230036, China
关键词:
厚朴内生真菌 小麦全蚀病 小麦全蚀病菌 酶活性
Keywords:
Magnolia officinalis wheat take-all Gaeumannomyces graminis enzyme activity
分类号:
S476
DOI:
10.3969/j.issn.1000-4440.2016.02.008
文献标志码:
A
摘要:
为了研究厚朴内生真菌HPFJ3对小麦全蚀病的生防作用,以厚朴内生真菌HPFJ3为试验菌株,小麦全蚀病菌为指示菌,通过离体平板对峙培养检测、光学显微镜观察,分析厚朴内生真菌HPFJ3与小麦全蚀病菌之间的相互作用; 利用盆栽接种试验,研究厚朴内生真菌HPFJ3对小麦全蚀病的防治效果以及对不同生育期的小麦植株根部多酚氧化酶(PPO)、过氧化物酶(POD)和苯丙氨酸解氨酶(PAL)活性的影响。结果表明,厚朴内生真菌HPFJ3能抑制小麦全蚀病菌的生长,造成小麦全蚀病菌菌丝节间缩短、变粗等畸形现象; 接种至小麦根际土壤中的厚朴内生真菌HPFJ3能增强小麦对全蚀病的抗性,其苗期小麦全蚀病的发病率为15.95%,显著低于未经厚朴内生真菌HPFJ3处理的小麦全蚀病的发病率(88.07%); 且经HPFJ3单独接种处理的小麦植株根部PPO、POD和PAL 3种防御酶活性在小麦5个生长时期均有所提高。
Abstract:
The endophytic fungus HPFJ3 with the potential of biocontrol activity against wheat take-all caused by Gaeumannomyces graminis were studied through the investigations of the interactions between HPFJ3 and G. graminis by dual culture experiment on PDA plates and enzymes activities in wheat root at different growth stages. The strain HPFJ3 had an antagonistic activity against G. graminis by causing mycelial malformation and breakage. The inoculation of HPFJ3 to the wheat rhizosphere could significantly improve the resistance to wheat take-all, and the incidence was only 15.95% at seedling stage, much lower than that of control group(88.07%). The activities of polyphenol oxidase(PPO), peroxidase(POD)and phenylalanine ammonla(PAL)in wheat roots treated with HPFJ3 were enhanced throughout the growth period.

参考文献/References:

[1] STONE J K, BACON C W, WHITE J F J. An overview of endophytic microbes: endophytism defined microbial endophytes[M]. New York: Marcel Dekker, 2000:3-29.
[2] PETINI O, ANDREWS J H, HIRANO S S. Fungal endophytes of tree leaves microbial ecology of tree leaves[M]. New York: Springer-verlag, 1991: 179-197.
[3] 邱观荣. 紫杉醇植物内生真菌来源及其生物多样性[J]. 国外医药(抗生素分册), 1998, 19(4): 265.
[4] WÄLI P R, HELANDER M, SALONIEMI I, et al. Variable effects of endophytic fungus on seedling establishment of fine fescues[J]. Oecologia, 2009, 152(1): 49-57.
[5] GHIMIRE S R, CHARLTON1 N D, CRAVEN K D. The mycorrhizal fungus, Sebacina vermifera, enhances seed germination and biomass production in switchgrass(Panicum virgatum L.)[J]. Bioenergy Research, 2009,102:51-58.
[6] CARUSO M, COLOMBO A L, FEDELI L, et al. Isolation of endophytic fungi and Actinomycetes taxane producers[J]. Annals of Microbiology, 2000, 50(1): 3-13.
[7] WALLER F, AEHATZ B, BALTRUSEHAT H, et al. The endophytic fungus Piriformospora indica reprograms barley to salt-stress tolerance, disease resistance, and higher yield[J]. Proceedings of the National Academy of Sciences of the United States of America, 2005, 102: 13386-13391.
[8] ISTIFADAH N, SALEEBA J A, MCGEE P A. Isolates of endophytic Chaetomium spp. inhibit the fungal pathogen Pyrenophora tritici-repentis in vitro[J]. Canadian Journal of Botany, 2006, 84(7): 1148-1155.
[9] ISTIFADAH N, MCGEE P A. Endophytic Chaetomium globosum reduce development of tan spot in wheat caused by Pyrenophora tritici-repentis[J]. Australasian Plant Pathology, 2006, 35(4): 411-418.
[10] 肇 莹,杨 镇,杨 涛,等. 植物内生菌醇提取物对草坪草抗旱性的影响[J].江苏农业学报,2015,31(1):39-44.
[11] 陶宏征,张建春,鲁海菊,等.抗枇杷根腐病病菌的6株枇杷主干内生真菌生物学特性研究[J].江苏农业科学,2015,43(9):166-168.
[12] 鲁海菊,董 梅,崔同敏,等.从枇杷内生真菌中筛选抗枇杷根腐病菌的活性菌株[J].江苏农业科学,2014,42(1):95-97.
[13] 刘亚光,李海英,杨庆凯. 大豆品种的抗病性与叶片内苯丙氨酸解氨酶活性关系的研究[J]. 大豆科学, 2002, 21(3):195-198.
[14] 秦国政,田世平,刘海波,等. 拮抗菌与病原菌处理对采后桃果实多酚氧化酶、过氧化物酶及苯丙氨酸解氨酶的诱导[J]. 中国农业科学, 2003, 36(1):89-93.
[15] 武志江,李业燕,王亚军,等. 百合枯萎病拮抗细菌的筛选、鉴定及其抑菌物质研究[J]. 微生物学通报, 2015, 42(7):1307-1320.
[16] 彭 娟. 小麦内生细菌对小麦全蚀病的生物防治研究[D]. 开封: 河南大学, 2008.
[17] 孙淑琴,李荣华,杨秀荣,等. 草坪草褐斑病菌粗毒素液对寄主叶片防御酶体系的影响[J]. 山东农业科学, 2012, 44(1): 87-90.
[18] RUIZ-SANCHEZ J, FLORES-BUSTAMANTE Z R, DENDOOVEN L, et al. A comparative study of Taxol production in liquid and solid-state fermentation with Nigrospora sp. a fungus isolated from Taxus globosa [J]. Journal of Applied Microbiology, 2010, 109(6):2144-2150.
[19] XU J, ALY A H, WRAY V, et al. Polyketide derivatives of endophytic fungus Pestalotiopsis sp. isolated from the Chinese mangrove plant Rhizophora mucronata[J]. Tetrahedron Letters, 2011, 52(1):21-25.
[20] LOPES A A, PUPO M T. Biosynthesis of aphidicolin proceeds via the mevalonate pathway in the endophytic fungus Nigrospora sphaerica[J]. Journal of The Brazilian Chemical Society, 2011, 22(1):80-85.
[21] 李 丽,翟梅枝,杨 惠,等. 核桃叶部内生真菌发酵产物抑菌活性及 GC-MS 分析[J].江苏农业科学,2014,42(10):292-295.
[22] TANAKA M, FUKUSHIMA T, TSUJINO Y, et al. Nigrosporins A and B, new phytotoxic and antibacterial metabolites produced by a fungus Nigrospora oryzae[J].Bioscience, Biotechnology and Biochemistry, 1997, 61(11): 1848-1852.
[23] 王国平,王丽薇. 一株罗汉松内生真菌及其活性代谢产物[J]. 中国生物防治学报, 2014, 30(2): 287-292.
[24] ZHAO J H, ZHANG Y L, WANG L W, et al. Bioactive secondary metabolites from Nigrospora sp. LLGLM003, an endophytic fungus of the medicinal plant Moringa oleifera Lam [J]. World Journal of Microbiology and Biotechnology, 2012, 28(5): 2107-2112.
[25] 李文峰. 利用小麦内生真菌生物防治小麦全蚀病研究[D]. 开封: 河南大学, 2006.
[26] 张 颖,王 刚,王云帆,等. 枯草芽孢杆菌B2-47对小麦全蚀病的防治及其病原的抑制作用[J]. 河南大学学报, 2006,36(1): 79-81.
[27] 刘秀娟,陈秀荣,杨成德,等. 生防芽孢杆菌与扑海因混配对番茄早疫病菌的抑制作用[J]. 甘肃农业大学学报, 2007,42(1): 49-53.
[28] HARMAN G E. Myths and dogmas of biocontrol: changes in peiceptions derived from research on Trichoderma harzianum T-22[J]. Plant Diseases, 2000, 84(4): 377-393.
[29] HOWELL C R. Mechanisms employed by Trichoderma species in the biological control of plant diseases: the history and evolution of current concepts[J]. Plant Diseases, 2002, 87(1): 4-10.

备注/Memo

备注/Memo:
收稿日期:2015-07-29 基金项目:浙江省自然科学基金项目(LY13C140005) 作者简介:姜华年(1965-),男,浙江遂昌人,硕士,副教授,主要从事植物栽培基础科学的教学与研究。(E-mail)jhn2585736@163.com
更新日期/Last Update: 2016-03-20