[1]梁郸娜,胡其靖,曹 磊,等.蚜虫侵染对黄瓜叶片中丙二醛含量及保护酶活性的影响[J].江苏农业学报,2016,(02):278-284.[doi:10.3969/j.issn.1000-4440.2016.02.007]
 LIANG Dan-na,HU Qi-jing,CAO Lei,et al.Effects of aphid(Aphis gossypii Glover)infestation on MDA content and protective enzymes activities in cucumber[J].,2016,(02):278-284.[doi:10.3969/j.issn.1000-4440.2016.02.007]
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蚜虫侵染对黄瓜叶片中丙二醛含量及保护酶活性的影响()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2016年02期
页码:
278-284
栏目:
植物保护
出版日期:
2016-03-20

文章信息/Info

Title:
Effects of aphid(Aphis gossypii Glover)infestation on MDA content and protective enzymes activities in cucumber
作者:
梁郸娜 胡其靖 曹 磊 宋琳琳 徐 强 齐晓花 周福才 陈学好
扬州大学园艺与植物保护学院,江苏 扬州 225009
Author(s):
LIANG Dan-na HU Qi-jing CAO Lei SONG Lin-lin XU Qiang QI Xiao-hua ZHOU Fu-cai CHEN Xue-hao
College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, China
关键词:
黄瓜 蚜虫 丙二醛 保护酶
Keywords:
cucumber aphid malondialdehyde protective enzyme
分类号:
S436.421.2+1
DOI:
10.3969/j.issn.1000-4440.2016.02.007
文献标志码:
A
摘要:
为了研究黄瓜抗蚜性与丙二醛(MDA)含量及保护酶活性的关系,选用抗蚜黄瓜品系EP6392和感蚜品系JY30为试验材料,在第1片真叶展平时,在每株的第1片真叶背面接种5头无翅成蚜作为处理,不接蚜虫的植株作为对照,测定黄瓜叶片中MDA含量和保护酶活性。结果表明:蚜虫接种后两品系的MDA含量和过氧化物酶(POD)、苯丙氨酸解氨酶(PAL)的活性显著高于对照。感蚜品系的 MDA含量始终极显著高于抗蚜品系; 蚜虫侵染后,抗蚜品系POD活性较感蚜品系上升迅速,抗蚜品系PAL的活性极显著高于感蚜品系。因此,MDA、POD和PAL参与了黄瓜对蚜虫侵染的防御调控。
Abstract:
To analyse the defensive role of MDA and protective enzymes against aphid in cucumber, cucumber lines EP6392(resistant)and JY30(susceptible)were measured for their MDA content and protective enzymes activities. Five apterous adult aphids were transferred to the back of the first true leaf of each cucumber plant as treatment and the seedling without aphids was set as control. After the inoculation, MDA content, and peroxidase(POD), phenylalanine ammonialyase (PAL)activities in cucumber seedlings were boosted in both lines. The MDA content in the susceptible line was significantly higher than that in the resistant line. POD activity of the resistant line was increased more rapidly than that in susceptible line. PAL activity in the resistant line was much higher than that in susceptible line after aphid infection. Therefore, MDA, POD and PAL may participate in the cucumber defense regulation against aphid infection.

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备注/Memo

备注/Memo:
收稿日期:2015-08-18 基金项目:国家重点基础研究发展计划(973计划)项目(2012CB113900) 作者简介:梁郸娜(1987-),女,四川江油人,博士研究生,主要从事黄瓜抗蚜性研究。(E-mail)liangdanna121423@163.com 通讯作者:陈学好,(E-mail)xhchen@yzu.edu.cn
更新日期/Last Update: 2016-03-20